Monitoring Heterocyst Isolation in Anabaena PCC 7119

  • Pedro Razquin
  • M. Teresa Bes
  • M. Luisa Peleato
  • Maria F. Fillat
Short Comunications


The purification of isolated and intact heterocysts is an essential step in the study and characterisation of their specific proteins. Therefore, a method for very successful heterocyst isolation from filamentous cyanobacteria, as monitored by measuring the presence of ferredoxin-NADP+ reductase activity during the isolation procedure has been developed.This is an improvement over the current lysozyme method in which damage could be caused to the heterocysts septum releasing soluble proteins. Frozen filaments should not be used for heterocysts isolation.


Anabaena heterocysts ferredoxin-NADP+ reductase 



ferredoxin-NADP+ reductase


glucose-6-phosphate dehydrogenase


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Copyright information

© Springer 2002

Authors and Affiliations

  • Pedro Razquin
    • 1
  • M. Teresa Bes
    • 1
  • M. Luisa Peleato
    • 1
  • Maria F. Fillat
    • 1
  1. 1.Departamento de Bioquimica y Biologia Molecular y CelularFacultad de Ciencias, Universidad de ZaragozaZaragozaSpain

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