Advertisement

Somatic Embryogenesis and Plantlet Regeneration of Melia azedarach L (Ghora Neem) from Cotyledonary Segments

  • Chitta Ranjan Deb
Short Communication

Abstract

Embryogenic calli were obtained within 3–4 weeks on MS medium supplemented with 3% sucrose, 200 mg I-1 caseinhydrolysate, and 3 mg l−1 each of 2,4-D and NAA from 3–4 days imbibed seeds. Heart-shaped embryos differentiated when growth regulators were withdrawn gradually. Quantification of somatic embryo formation showed a direct relationship between initial response, somatic embryo formation and its subsequent germination. The somatic embryos germinated into emblings with 90% conversion frequency on MS medium containing 2% sucrose.

Key words

cotyledonary segments ghora neem Melia azedarach somatic embryo somatic seedling/embling 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Srivastava SD, J Nat Prod, 49 (1986) 56.PubMedCrossRefGoogle Scholar
  2. 2.
    Murthy BNS & Saxena PK, Plant Cell Rep, 17 (1998) 469.CrossRefGoogle Scholar
  3. 3.
    Thakur R, Rao PS & Bapat VA, Plant Cell Rep, 18 (1998) 127.CrossRefGoogle Scholar
  4. 4.
    Sujatha M & Chandran K, J Exp Biol, 35 (1997) 787.Google Scholar
  5. 5.
    Murashige T & Skoog F, Physiol Plant, 15 (1962) 473.CrossRefGoogle Scholar
  6. 6.
    Chan JL, Saenz L, Talavera C, Hornung R, Robert M & Oropeza , Plant Cell Rep, 17 (1998) 515.CrossRefGoogle Scholar
  7. 7.
    Tiwari V, Deo Singh B & Nath Tiwari K, Plant Cell Rep, 17 (1998) 538.CrossRefGoogle Scholar
  8. 8.
    Ishii Y, Takamura T, Goi M & Tanaka M, Plant Cell Rep, 17 (1998) 446.CrossRefGoogle Scholar
  9. 9.
    Chengalrayan K, Mhaske VB & Hazra S, Plant Cell Rep, 17 (1998) 522.CrossRefGoogle Scholar
  10. 10.
    Gautam VK, Nanda K & Gupta SC, Plant Cell Tiss Org Cult, 34 (1993) 13.CrossRefGoogle Scholar

Copyright information

© Springer 2001

Authors and Affiliations

  • Chitta Ranjan Deb
    • 1
  1. 1.Department of BotanyNagaland UniversityNagalandIndia

Personalised recommendations