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Sequence Analysis of a Plastidial Omega-3 Desaturase Gene from Brassica juncea

  • Renu Garg
  • I. M. Santha
  • M. L. Lodha
  • S. L. Mehta
Article

Abstract

A genomic clone (λg2) encoding a plastidial omega-3 desaturase has been isolated from a genomic library of Brassica juncea cv Pusa Bold and sequenced after subcloning. A continuous stretch of 3866 by of the clone sequenced showed homology with omega-3 desaturase from other plant sources but maximum homology was with that of a plastidial omega-3 fad 7 gene. This sequence designated as Bjgfad7 includes 1381 by 5’ untranslated region, 273 by 3’ untranslated region along with an open reading frame of 1290 by interrupted by 7 introns. The 5’ untranslated region contains putative cis elements responsible for seed specificity, light responsive and stress-inducible expression besides minimal promoter elements.

Key words

omega-3 desaturase genomic sequence promoter cis elements Brassica juncea 

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Copyright information

© Springer 2001

Authors and Affiliations

  • Renu Garg
    • 1
  • I. M. Santha
    • 1
  • M. L. Lodha
    • 1
  • S. L. Mehta
    • 1
  1. 1.Division of BiochemistryIndian Agricultural Research InstituteNew DelhiIndia

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