Abstract
Genomic DNA clones coding for legumin storage protein were isolated from chickpea (Cicer arietinum L.) subgenomic library using pea legumin cDNAs as a probe. The complete nucleotide sequence of the legumin gene (leg3) was determined. Sequence analysis indicated that leg3 contains 2022 bp long structural gene interrupted by three small introns. The gene encodes a polypeptide of 496 amino acids including a signal peptide of 21 amino acid residues and a-b subunit cleavage site. Leg3 polypeptide is rich in amide amino acids and contains 5 methionine and 6 cysteine amino acids. DNA sequence comparison showed about 70–80% identity with legumin genes from pea, soybean and broad bean. The 5’ flanking region contains regulatory elements such as TATA and legumin box and 3’ region contains a stop codon and two polyadenylation signals. Southern hybridisation indicated the presence of multiple copies of /eg3in chickpea and estimated about 10–12 copies per haploid genome. Northern blot hybridisation showed that leg3 expresses in developing seed and not in other tissues of chickpea indicating temporal and tissue-specific expression of this gene.
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References
Derbyshire E, Wright DJ & Boulter D, Phytochemistry, 15, (1976), 3.
Casey R, Domoney C & Ellis N, Oxford survey of plant molecular and cell biology, 3, (1986), 1.
Shotwell MA & Larkins BA, In The Biochemistry of plants, Vol 15 (PK Stumpf, EE Conn, Editors), Academic Press, London (1989) pp 297–345.
Mittal M & Koundal KR, Ind J Agric Biochem, 4, (1990), 1.
Mandaokar AD, Kansal R, Koundal KR & Bansal HC, J Plant Biochem Biotech, 2, (1993), 35.
Dellaporta SL, Wood J & Hicks JB, Plant Mol Biol Report 1 (1983) 19.
Koundal KR, Mehta SL & Sharma RP, Ind J exp Biol. 27, (1989), 858.
Stephen D, Jones C & Schofield JP, Nucl Acids Res, 18 1990) 7463.
Chomczynski P & Sacchl N, Anal Biochem, 162, (1989), 156.
Mandaokar AD & Koundal KR, Ind J exp Biol, 34, (1996), 496.
Henikoff S, Gene. 28 (1984), 351.
Sanger F, Nicklen S & Coulsen AN, Proc Natl Acad Sci, USA, 74, (1977), 5463.
Sambrook J, Fritsch ZF & Maniatis T, Molecular cloning: A laboratory manual, 2nd Edn, Cold Spring Harbor Laboratory, New York (1989).
Lycett GW, Croy RRD, Shirsat AH & Boulter D, Nucl Acids Res, 12, (1984a), 4493.
Lycett GW, Delauney AJ, Zhao W, Gatehouse JA, Croy RRD & Boulter D, Plant Mol Biol. 3 (1984b), 91.
Heim U, Schubert R, Baumlein H & Wobus U, Plant Mol Biol. 13, (1989), 653.
Nielson NC, Dickinson CD, Sho T, Than VH, Scallon BJ, Fischer RL, Sims L, Drews GN & Goldberg RB, Plant Cell. 1, (1989) 313.
Dickinson CD, Evan RP & Nielsen NC. Nucl Acids Res, 16, (1988) 371.
Baumlein H, Nagy I, Villarood R, Inze D & Wobus U, The Plant J, 2, (1992), 233.
Joshi CP, Nucl Acids Res, 15, (1987), 9627.
Brown JWS, Nucl Acids Res, 14, (1986), 9549.
Gatehouse JA, Evan IM, Croy RRD & Boulter D, Phil Trans R Soc Lond Ser, B 314, (1986), 367.
Nair L & Koundal KR, J Plant Biochem Biotech 2 (1993), 29.
Kumar LD, Mandaokar AD & Koundal KR, Ind J exp Biol, 37, (1999), 1117
Yamauchi D & Minamikawak T, J Plant Res, 111, (1998), 1.
Koundal KR, Plant Physiol Biochem, 10, (1983), 60.
Domoney C & Casey R, Nucl Acids Res, 13, (1985), 687.
Shutov AD, Kakhovskaya IlA, Brown H & Muntz K, J Mol Evol, 41, (1995), 1057.
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Mandaokar, A.D., Koundal, K.R. Cloning and Characterization of Legumin Storage Protein Gene of Chickpea (Cicer arietinum L). J. Plant Biochem. Biotechnol. 9, 7–12 (2000). https://doi.org/10.1007/BF03263075
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DOI: https://doi.org/10.1007/BF03263075