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Purification and Characterization of Protease Inhibitor from Rice Bean (Vigna umbellata T.) Seeds

  • Sanjay Maggo
  • Sarla P. Malhotra
  • Kamal Dhawan
  • Randhir Singh
Short Communication

Abstract

A Protease inhibitor from seeds of rice bean has been purified to apparent homogeneity as judged by native-PAGE with about 29% recovery using ammonium sulfate fractionation, ion exchange chromatography on DEAE-cellulose, and gel filtration through Sephadex G-100. The purified preparation with molecular weight of 16.8 kD was found to be a monomer as revealed by SDS-PAGE under reducing and non-reducing conditions. The purified inhibitor in solution was stable upto 40°C. However, it lost its activity gradually and was completely inactive when heated at 100°C for 2 h and 125°C for 1 h. Heating at 125°C changed the conformation of the inhibitor as was evident from the altered UV spectrum compared to that of native. It had two pH optima at pH 6.0 and at 10.0 and was stable over a wide range of pH (pH 3.0 to 10.0). It lost its activity on exposure to 2-mercaptoethanol, indicating the role of S-S linkages in maintaining the three dimensional structure of the protein inhibitor. The inhibitor was completely inactive towards papain, while it inhibited pepsin only slightly. Trypsin and chymotrypsin were inhibited upto the extent of 60% and 30%, respectively. Trypsin inhibition was of non-competitive type with dissociation constant for the enzyme-inhibitor complex in the region of 2.07 mg ml-1. The rice bean inhibitor appears to be of Bowman-Birk type as it has molecular weight lower than that generally observed for Kunitz type inhibitors and seems to be double headed - a characteristic specific of Bowman - Birk type inhibitor.

Key words

protease inhibitor rice bean purification characterization 

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Copyright information

© Springer-Verlag 1999

Authors and Affiliations

  • Sanjay Maggo
    • 1
  • Sarla P. Malhotra
    • 1
  • Kamal Dhawan
    • 1
  • Randhir Singh
    • 1
  1. 1.Department of BiochemistryCCS Haryana Agricultural UniversityHisarIndia

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