Detection of nif Genes in Nitrogen-Fixing Bacterial Isolate from Tomato (Lycopersicon esculentum Mill cv Pusa Ruby)

  • Sukhada Mohandas
  • M. S. Shailal
Short Communication


Nitrogen-fixing bacterial isolate from the intercellular spaces of tomato root cortical cells was studied for the location of nif genes on the chromosomal or plasmid DNA. The bacterial isolate showed two plasmids of approximate molecular sizes of 220 and 120 kb. Klebsiella pneumoniae nif HDK probe hybridized with the chromosomal DNA and not with the plasmid DNA thereby showing that nif genes are localised on the chromosomal DNA.

Key words

chromosomal DNA nif HDK probe hybridization 


  1. 1.
    Dobereiner J, Day JM, Newton VIE & Nyman CJ, Proc International Symp Nitrogen fixation, Washington State University Press, Pullman (1975) p 518.Google Scholar
  2. 2.
    Pohlman DA & Mc Coll CG, Plant and Soil, 68 (1982) 341.CrossRefGoogle Scholar
  3. 3.
    Dart PJ & Subba Rao RC, In Associated nitrogen fixation Vol I (PB Vose, AP Rushel, Editors), CRC Press, Florida (1981) P 160.Google Scholar
  4. 4.
    Patriquin DG & Dobereiner J, Can J Microbiol, 24 (1978) 734.PubMedCrossRefGoogle Scholar
  5. 5.
    Hill WA, Hill PB, Grossman SM & Stevens C, Can J Microbiol, 29 (1983) 860.CrossRefGoogle Scholar
  6. 6.
    Mohandas Sukhada, Plant and Soil, 107 (1988) 219.CrossRefGoogle Scholar
  7. 7.
    Elmerich C & Franche C, In Azospirillum genetics, physiology, ecology (Klingmiller, Editor), Eirkhauser, Basset (1982) p 9.Google Scholar
  8. 8.
    Plazinski J, Dart PJ & Rolfe BG, J Bacterial, 155 (1983) 1429.Google Scholar
  9. 9.
    Maniatis T, Fritsch EF & Sambrook J, Molecular cloning: A laboratory manual, Cold Spring Harbour Laboratory (1982).Google Scholar

Copyright information

© Springer 1996

Authors and Affiliations

  • Sukhada Mohandas
    • 1
  • M. S. Shailal
    • 2
  1. 1.Division of BiotechnologyIndian Institute of Horticultural Research, Hessaraghatta Lake P.O.BangaloreIndia
  2. 2.Department of Microbiology & Cell BiologyIndian Institute of ScienceBangaloreIndia

Personalised recommendations