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Sequence Homology of Two Indian Isolates of Cucumber Mosaic Virus at N-terminal Amino Acid Sequences of the Coat Protein Gene with CMV Strains

  • S. K. Raj
  • Q. M. R. Haq
  • K. M. Srivastava
  • B. P. Singh
Article

Abstract

The cDNAs representing coat protein gene (RNA4) of CMV-CR (Dianthus barbatus strain) and CMV-P (Physalis minima strain) were synthesized using random primers and cloned into Bluescript II KS (+) phagemid at Sma I site. The clones were partially sequenced and compared with published nucleotide sequences of selected CMV-strains. Comparison of 249 nucleotide sequences of 5′ coding region of CP gene and their putative products revealed the possibility of placing the strains into sub-group I of CMV.

Key words

cucumber mosaic virus coat protein gene sequence homology 

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • S. K. Raj
    • 1
  • Q. M. R. Haq
    • 1
  • K. M. Srivastava
    • 1
  • B. P. Singh
    • 1
  1. 1.Plant Virus LaboratoryNational Botanical Research InstituteLucknowIndia

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