Callus Cultures from Zygotic Embryos of Costus speciosus and Their Morphogenetic Responses

  • Archana Roy
  • Amita Pal


Soft, nodular and hard types of calli were initiated on mature zygotic embryo explants of two tetraploid clones of Costus speciosus, of which, only the hard calli were amenable to morphogenetic responses. The two clones differed in their growth regulator requirements both for the initiation of calli and for shoot regeneration. De novo formation of both shoot bud meristems and somatic embryoids were observed. Latter were encased partially or fUlly by coleoptilar sheath. Embryoids could be isolated as discrete units. On maturity, a stock like appendage developed from the base and finally embryoids got detached from the subtending tissue. Both shoot-bud meristems and somatic embryoids developed into complete plantlets, the former upon sequential transfer of calli on Schenk and Hildebrandt’s (SH) basal medium containing lower levels of growth hormones, while the latter only on basal medium. These culture regenerants were subsequently transferred to the field. The morphogenetic behaviour of these two tetraploid clones reflects their marked genotypic difference inspite of their same ploidy status.

Key words

embryogenesis organogenesis shoot-bud somatic embryoid zygotic embryo 





callus induction medium


2,4-dichlorophenoxyacetic acid


indoleacetic acid


α-naphthaleneacetic acid


Schenk and Hildebrandt


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Nagendra P & Abraham PZ, Curr Sci, 50 (1981) 26.Google Scholar
  2. 2.
    Chaturvedi HC, Misra P & Jain M, Plant Sci Lett, 3 (1984) 67.Google Scholar
  3. 3.
    Pal A, Cell & Chr Res, 6 (1983) 68.Google Scholar
  4. 4.
    Jain M & Chaturvedi HC, Planta Medica, 5 (1985) 462.Google Scholar
  5. 5.
    Pal A & Sharma AK, In Proc CaSTED Symp on Tissue Culture of Economically Important Plants (AN Rao, Editor), Singapore (1981) pp 85–89.Google Scholar
  6. 6.
    Schenk RU & Hildebrandt AC, Can J Bot, 50 (1972) 199.CrossRefGoogle Scholar
  7. 7.
    Gomez KA & Gomez AA, In Statistical procedures for agricultural research, Wiley International Publication. John Wiley & Sons, Inc., New York (1984).Google Scholar
  8. 8.
    Johansen DA, Plant microtechnique, McGraw-Hili Book Company Inc., New York (1940).Google Scholar
  9. 9.
    Eapen S & Rao PS, Plant Cell Rep, 1 (1982) 215.CrossRefGoogle Scholar
  10. 10.
    McDonnell RE & Conger BV, Crop Sci, 24 (1984) 573.CrossRefGoogle Scholar
  11. 11.
    Lupotto E, Ann Bot, 54 (1984) 523.Google Scholar
  12. 12.
    Roy A & Pal A, Plant Cell Rep, 10 (1991) 525.CrossRefGoogle Scholar

Copyright information

© Springer 1995

Authors and Affiliations

  • Archana Roy
    • 1
  • Amita Pal
    • 1
  1. 1.Plant Molecular and Cellular Genetics UnitBose InstituteCalcuttaIndia

Personalised recommendations