The Study of Apparent Bacterial Disappearance and Slow-Growing Bacteria in Urine Culture by the Dip-Slide Method in Evaluating the Efficacy of Antimicrobial Drugs
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15 patients with complicated urinary tract infections were treated with 500mg/500mg of imipenem/cilastatin (IPM/CS) twice a day for 5 days by intravenous infusion. Urine samples collected from the patients were cultured on dip-slides every day. The effect of the antibiotics in the urine and the problems of slow-growing bacteria were evaluated by comparing the results of untreated urine samples spread on dip-slides with those of urine samples treated by washing.
The results were as follows: (a) the date of bacterial disappearance tended to be delayed when the antibiotic in the urine samples was removed by washing; (b) more slow-growing bacteria were isolated from washed samples than untreated samples; and (c) Enterococcus faecalis, Serratia marcescens and Pseudomonas aeruginosa were isolated mainly as slow-growing bacteria. It is suggested that these bacteria may cause a relapse of infection after drug treatment, even though culture methods during and up to 24 hours after treatment show no bacteria in the urine.
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