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Molecular Diagnosis & Therapy

, Volume 10, Issue 4, pp 231–237 | Cite as

Digital Karyotyping

An Update of its Applications in Cancer
  • Ritu Salani
  • Chao-Ling Chang
  • Leslie Cope
  • Tian-Li Wang
Cancer

Abstract

DNA copy number alterations, including entire chromosomal changes and small interstitial DNA amplifications and deletions, characterize the development of cancer. These changes usually affect the expression of target genes and subsequently the function of the target proteins. Since the completion of the human genome project, the capacity to comprehensively analyze the human cancer genome has expanded significantly. Techniques such as digital karyotyping have been developed to allow for the detection of DNA copy number alterations in cancer at the whole-genome scale. When compared with conventional methods such as spectral karyotyping, representational difference analysis, comparative genomic hybridization (CGH), or the more recent array CGH; digital karyotyping provides an evaluation of copy number of genetic material at higher resolution. Digital karyotyping has therefore promised to enhance our understanding of the cancer genome. This article provides an overview of digital karyotyping including the principle of the technology and its applications in identifying potential oncogenes and tumor suppressor genes.

Keywords

Medulloblastoma Homozygous Deletion Array Comparative Genomic Hybridization Single Nucleotide Polymorphism Array Ovarian Serous Carcinoma 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This study was supported by the US Department of Defense grant (W81XWH-05-1-0004).

The authors have no conflicts of interest that are directly relevant to the content of this review.

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Copyright information

© Adis Data Information BV 2006

Authors and Affiliations

  • Ritu Salani
    • 1
    • 2
  • Chao-Ling Chang
    • 2
  • Leslie Cope
    • 2
  • Tian-Li Wang
    • 1
    • 2
  1. 1.Department of GynecologyJohns Hopkins University School of MedicineBaltimoreUSA
  2. 2.Department of OncologyJohns Hopkins University School of MedicineBaltimoreUSA

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