Abstract
Background and Objective: By current WHO criteria, most — though not all — cases of hematolymphoid neoplasm can be diagnosed immunomorphologically, diminishing the role of molecular tests for lymphoid antigen receptor clonality in lymphoma diagnosis. Hence, our objective was to glean immunomorphological and molecular correlates from hematolymphoid neoplasms that had remained unresolvable without diagnostic molecular input.
Methods: Thirty-five such cases were reviewed histologically and with standard immunoperoxidases. In situ hybridization for Epstein-Barr virus (EBV)-encoded RNAs (EBER) was performed on selected cases. PCR amplification of genes encoding T-cell receptors (TcR) and immunoglobulin heavy chains (IgH) [TR and IGH genes, respectively] was performed on whole tissue in all cases, and on microdissected cells in two cases.
Results: Twenty-five cases (71%) requiring diagnostic molecular genotyping had some form of peripheral T-cell lymphoma (PTCL). Twenty (80%) of these were complicated by a proliferation of B-lineage cells, either within the same tissue (‘syntopic’) as large B cells (LBC) or Reed-Sternberg (RS)-like cells (17 cases), florid lymphoid hyperplasia (two cases, one also with syntopic LBC) or monotypic plasma cells (one case), or at a separate (‘metatopic’) site as a B-cell lymphoma (two cases, one of which also had syntopic LBC) or Hodgkin lymphoma (HL; one case, also showing syntopic LBC). Fifteen (75%) of these 20 PTCLs with B-lineage proliferation yielded monoclonal TR gene rearrangements, and only two (10%) showed IGH monoclonality, which was transient in one case. Three (18%) of the PTCLs with LBC had originally been misinterpreted as some form of HL. Conversely, of the remaining cases, three of four (75%) that had been diagnosed initially as some form of large cell non-HL (NHL), including two of three that were called ‘anaplastic’, had to be revised to grade II/syncytial nodular sclerosing (NS) HL, yielding polyclonal TcRγ gene (TRG) rearrangements, with one case, in addition, disclosing a biallelic clonal IGH gene rearrangement that excluded anaplastic large cell lymphoma.
Discussion/Conclusion: Paradoxically, monoclonality of TR rather than IGH gene rearrangement may more often be detectable in a predominantly dispersed (‘hodgkinoid’), large B-lineage cell proliferation, consistent with release from immune regulation in the milieu of impaired immunosurveillance within a PTCL. This is compounded by the difficulty in ascertaining clonal IGH gene rearrangements resulting from (1) poor consensus primer hybridization due to somatic hypermutations, and (2) ‘dilution’ in a T-cell-rich milieu. These same difficulties also account for the long-elusive identification of the RS cell lineage. Conversely, anaplastic lymphoma, which is of non-B lineage, may be mimicked by NSHL, which is of B lineage.
Similar content being viewed by others
Notes
Please refer to the table in the supplementary material to this article, which is listed as ‘ArticlePlus’ and can be found with the electronic version of this article on AdisOnline (http://moleculardiagnosistherapy.adisonline.com).
References
Jaffe ES, Harris NL, Stein H, et al. Pathology and genetics: tumors of haematopoeitic and lymphoid tissues. World Health Organization classification of tumours. Lyon: International Agency for Research on Cancer, 2001
Gulley ML. Antigen receptor gene rearrangements. In: Leonard DGB, editor. Diagnostic molecular pathology: major problems in pathology. Vol. 41. Philadelphia (PA): Saunders, 2003: 118–24
Pan L, Cesarman E, Knowles DM. Antigen receptor genes: structure, function and genetic analysis of their rearrangements. In: Knowles DM, editor. Neoplastic hematopathology. Philadelphia (PA): Williams and Wilkins, 2001: 307–28
Antigen receptor genes and analysis of their rearrangements. In: Warnke RA, Weiss LM, Chan JKC, et al., editors. Tumors of the lymph nodes and spleen: atlas of tumor pathology, 3rd series, fascicle 14. Washington DC: US Armed Forces Institute of Pathology, 1994: 31–4
Evens AM, Gartenhaus RB. Treatment of T-cell non-Hodgkin’s lymphoma. Curr Treat Options Oncol 2004 Aug; 5(4): 289–303
Gallamini A, Stelitano C, Calvi R, et al. Peripheral T-cell lymphoma unspecified (PTCL-U): a new prognostic model from a retrospective multicentric clinical study. Blood 2004 Apr; 103(7): 2474–9
Savage KJ, Chhanabhai M, Gascoyne RD, et al. Characterization of peripheral T-cell lymphomas in a single North American institution by the WHO classification. Ann Oncol 2004 Oct; 15(10): 1467–75
Sehn LH, Connors JM. Treatment of aggressive non-Hodgkin’s lymphoma: a north American perspective. Oncology (Williston Park) 2005 Apr; 19(4 Suppl. 1): 26–34
Coiffier B, Reyes F. Groupe d’Etude des Lymphomes de l’Adulte. Best treatment of aggressive non-Hodgkin’s lymphoma: a French perspective. Oncology (Williston Park) 2005 Apr; 19(4 Suppl. 1): 7–15
Fisher RI, Miller TP, O’Connor OA. Diffuse aggressive lymphoma. Hematology (Am Soc Hematol Educ Program) 2004; 2004: 221–36
Younes A. New treatment strategies for aggressive lymphoma. Semin Oncol 2004 Dec; 31(6 Suppl. 15): 10–3
Coiffier B. Effective immunochemotherapy for aggressive non-Hodgkin’s lymphoma. Semin Oncol 2004 Feb; 31(1 Suppl. 2): 7–11
Kadin ME. Hodgkin’’s disease: cell of origin, immunobiology and pathogenesis. In: Knowles DM, editor. Neoplastic hematopathology. Philadelphia (PA): Williams and Wilkins, 2001: 667–90
Hertel CB, Zhou XG, Hamilton-Dutoit SJ, et al. Loss of B cell identity correlates with loss of B cell-specific transcription factors in Hodgkin/Reed-Sternberg cells of classical Hodgkin lymphoma. Oncogene 2000; 21: 4908–20
Marafioti T, Hummel M, Foss HD, et al. Hodgkin and Reed-Sternberg cells represent an expansion of a single clone originating from a germinal center B-cell with functional immunoglobulin gene rearrangements but defective immunoglobulin transcription. Blood 2000; 95: 1443–50
Theil J, Laumen H, Marafioti T, et al. Defective octamer-dependent transcription is responsible for silenced immunoglobulin transcription in Reed-Sternberg cells. Blood 2001 May 15; 97(10): 3191–6
Harris NL, Jaffe ES, Stein H, et al. A revised European-American classification of lymphoid neoplasms: a proposal from the international lymphoma study group. Blood 1994; 84(5): 1361–92
Jaffe ES. Anaplastic large cell lymphoma: the shifting sands of diagnostic hematopathology. Mod Pathol 2001; 14(3): 219–28
Weisenburger DD, Anderson JR, Diebold J, et al. Systemic anaplastic large-cell lymphoma: results from the non-Hodgkin’s lymphoma classification project. Am J Hematol 2001; 67(3): 172–8
Tan LHC, Chong SM. Grade II nodular sclerosing Hodgkin lymphoma commonly mimics non-Hodgkin lymphomas with anaplastic morphology: a pathologic review of 42 cases reclassified by current WHO criteria [abstract]. Mod Pathol 2003 Jan; 16(1): 255A
Tan LHC, Do E, Tan SY, et al. Multi-lineage interrogation of the performance characteristics of a split-signal fluorescence in situ hybridization probe (FISH) for Anaplastic Lymphoma Kinase gene rearrangement: a study of 101 cases characterized by immunohistomorphology on fixed archival tissue. Mol Diagn 2004; 8(4): 213–29
Tan LHC, Do E, Chong SM, et al. Detection of ALK gene rearrangements in formalin-fixed, paraffin-embedded tissue using a fluorescence in-situ hybridization (FISH) probe: a search for optimum conditions of tissue archiving and preparation for FISH. Mol Diagn 2003 Mar; 7(1): 27–33
Chang TL, Salto-Tellez M, Thamboo TP, et al. Diagnostic validation of capillary electrophoresis analysis of T-cell receptor γ-chain gene rearrangements: prediction of malignant transformation of cutaneous T-cell lymphoproliferative disorders. Clin Chemistry 2003; 49(3): 513–5
Sioutos N, Bagg A, Michaud GY, et al. Polymerase chain reaction versus Southern blot hybridization: detection of immunoglobulin heavy-chain gene rearrangements. Diagn Mol Pathol 1995 Mar; 4(1): 8–13
McCarthy KP, Sloane JP, Kabarowski JHS, et al. The rapid detection of clonal T-cell proliferation in patients with lymphoid disorders. Am J Pathol 1991 Apr; 138(4): 821–8
Classic Hodgkin’s disease. In: Warnke RA, Weiss LM, Chan JKC, et al., editors. Tumors of the lymph nodes and spleen: atlas of tumor pathology, 3rd series, fascicle 14. Washington DC: US Armed Forces Institute of Pathology, 1994: 227–314
Achten R, Verhoef G, Vanuytsel L, et al. Histiocyte-rich, T-cell-rich B-cell lymphoma: a distinct diffuse large B-cell lymphoma subtype showing characteristic morphologic and immunophenotypic features. Histopathology 2002 Jan; 40(1): 31–45
Achten R, Verhoef G, Vanuytsel L, et al. T-cell/histiocyte-rich large B-cell lymphoma: a distinct clinicopathologic entity. J Clin Oncol 2002 Mar 1; 20(5): 1269–77
Lim MS, Beaty M, Sorbara L, et al. T-cell/histiocyte-rich large B-cell lymphoma: a heterogeneous entity with derivation from germinal center B cells. Am J Surg Pathol 2002 Nov; 26(11): 1458–66
Ramsay AD, Smith WJ, Isaacson PG. T-cell-rich B-cell lymphoma. Am J Surg Pathol 1988 Jun; 12(6): 433–43
Lorenzen J, Li G, Zhao-Hohn M, et al. Angioimmunoblastic lymphadenopathy type of T-cell lymphoma and angioimmunoblastic lymphadenopathy: a clinicopathological and molecular biological study of 13 Chinese patients using polymerase chain reaction and paraffin-embedded tissues. Virchows Arch 1994; 424(6): 593–600
Jones D, Jorgensen JL, Shahsafaei A, et al. Characteristic proliferations of reticular and dendritic cells in angioimmunoblastic lymphoma. Am J Surg Pathol 1998 Aug; 22(8): 956–64
Lee S-S, Rudiger T, Odenwald T, et al. Angioimmunoblastic T-cell lymphoma is derived from mature T-helper cells with varying expression and loss of detectable CD4. Int J Cancer 2003; 103: 12–20
Attygalle A, Al-Jehani R, Diss TC, et al. Neoplastic T cells in angioimmunoblastic T-cell lymphoma express CD10. Blood 2002; 99(2): 627–33
Kojima M, Nakamura S, Itoh H, et al. Angioimmunoblastic T-cell lymphoma with hyperplastic germinal centers: a clinicopathological and immunohistochemical study of 10 cases. APMIS 2001; 109: 699–706
Steinhoff M, Hummel M, Assaf C, et al. Cutaneous T cell lymphoma and classic Hodgkin lymphoma of B cell type within a single lymph node: composite lymphoma. J Clin Pathol 2004 Mar; 57(3): 329–31
Quintanilla-Martinez L, Fend F, Moguel LR, et al. Peripheral T-cell lymphoma with Reed-Sternberg-like cells of B-cell phenotype and genotype associated with Epstein-Barr virus infection. Am J Surg Pathol 1999; 23(10): 1233–40
Higgins JP, van de Rijn M, Jones CD, et al. Peripheral T-cell lymphoma complicated by a proliferation of large B cells. Am J Clin Pathol 2000; 114(2): 236–47
Schwarting R, Gerdes J, Durkop H, et al. Ber-H2: a new anti-Ki-1 (CD30) monoclonal antibody directed at a formol-resistant epitope. Blood 1989 Oct; 74(5): 1678–89
Piris M, Brown DC, Gatter KC, et al. CD30 expression in non-Hodgkin’s lymphoma. Histopathology 1990 Sep; 17(3): 211–8
Lukes RL, Tindle BH. Immunoblastic lymphadenopathy: a hyperimmune entity resembling Hodgkin’s disease. N Engl J Med 1975 Jan 2; 292(1): 1–8
Knecht H, Berger C, Rothenberger S, et al. The role of Epstein-Barr virus in neoplastic transformation. Oncology 2001; 60: 289–302
Lome-Maldonado C, Canioni D, Hermine O, et al. Angio-immunoblastic T cell lymphoma (AILD-TL) rich in large B cells and associated with Epstein-Barr virus infection: a different subtype of AILD-TL? Leukemia 2002; 16: 2134–41
Zettl A, Lee SS, Rudiger T, et al. Epstein-Barr virus-associated B-cell lymphoproliferative disorders in angioimmunoblastic T-cell lymphoma and peripheral T-cell lymphoma, unspecified. Am J Clin Pathol 2002; 117(3): 368–79
Ho JWY, Ho FSC, Chan ACL, et al. Frequent detection of Epstein-Barr virus-infected B cells in peripheral T-cell lymphomas. J Pathol 1998; 185: 79–85
Nakamura S, Sasajima Y, Koshikawa T, et al. Angioimmunoblastic T-cell lymphoma (angioimmunoblastic lymphadenopathy with dysproteinemia [AILD]-type T-cell lymphoma) followed by Hodgkin’s disease associated with Epstein-Barr virus. Pathol Int 1995; 45(120): 958–64
Willenbrock K, Roes A, Seidl C, et al. Analysis of T-cell subpopulations in T-cell non-Hodgkin’s lymphoma of angioimmunoblastic lymphadenopathy with dysproteinemia type by single target gene amplification of T cell receptor-β gene rearrangements. Am J Pathol 2001; 158(5): 1851–7
Smith JL, Hodges E, Quin CT, et al. Frequent T and B cell oligoclones in histologically and immunophenotypically characterized angioimmunoblastic lymphadenopathy. Am J Pathol 2000 Feb; 156(2): 661–9
Diss TC, Watts M, Pan LX, et al. The polymerase chain reaction in the detection of monoclonality in T-cell lymphomas. J Clin Pathol 1995; 48: 1045–50
Polliack A, Lugassy G. Autoimmunity and auto-immune syndromes associated with and preceding the development of lymphoproliferative disorders. Leukemia 1992; 6Suppl. 4: 152–4
Pavlidis NA, Klouvas G, Tsokos M, et al. Cutaneous lymphocytic vasculopathy in lymphoproliferative disorders: a paraneoplastic lymphocytic vasculitis of the skin. Leuk Lymphoma 1995; 16(5-6): 477–82
Yataganas X, Papadimitriou C, Pangalis G, et al. Angio-immunoblastic lymphadenopathy terminating as Hodgkin’s disease. Cancer 1977 May; 39(5): 2183–9
Diss TC, Peng H, Wotherspoon AC, et al. Detection of monoclonality in low-grade B-cell lymphomas using the polymerase chain reaction is dependent on primer selection and lymphoma type. J Pathol 1993 Mar; 169(3): 291–5
Nakamura N, Kuze T, Hashimoto Y, et al. Analysis of the immunoglobulin heavy chain gene variable region of 101 cases with peripheral B cell neoplasms and B cell chronic lymphocytic leukemia in the Japanese population. Pathol Int 1999 Jul; 49(7): 595–600
Stein H, Foss HD, Durkop H, et al. CD30+ anaplastic large cell lymphoma: a review of its histopathologic, genetic, and clinical features. Blood 2000 Dec 1; 96(12): 3681–95
Foss HD, Reusch R, Demel G, et al. Frequent expression of the B-cell-specific activator protein in Reed-Sternberg cells of classical Hodgkin’s disease provides further evidence for its B-cell origin. Blood 1999 Nov 1; 94(9): 3108–13
Delsol G, Lamant L, Mariame B, et al. A new subtype of large B-cell lymphoma expressing the ALK kinase and lacking the 2; 5 translocation. Blood 1997 Mar 1; 89(5): 1483–90
Gascoyne RD, Lamant L, Martin-Suberto JI, et al. ALK-positive diffuse large B-cell lymphoma is associated with Clathrin-ALK rearrangements: report of 6 cases. Blood 2003 Oct; 102(7): 2568–73
De Paepe P, Baens M, van Krieken H, et al. ALK activation by the CLTC-ALK fusion is a recurrent event in large B-cell lymphoma. Blood 2003 Oct; 102(7): 2638–41
Onciu M, Behm FG, Downing JR, et al. ALK-positive plasmablastic B-cell lymphoma with expression of the NPM-ALK fusion transcript: report of 2 cases. Blood 2003 Oct; 102(7): 2642–4
Falini B, Pulford K, Pucciarini A, et al. Lymphomas expressing ALK fusion protein(s) other than NPM-ALK. Blood 1999 Nov 15; 94(10): 3509–15
Pulford K, Lamant L, Morris SW, et al. Detection of anaplastic lymphoma kinase (ALK) and nucleolar protein nucleophosmin (NPM)-ALK proteins in normal and neoplastic cells with the monoclonal antibody ALK1. Blood 1997 Feb; 89(4): 1394–404
Tan LHC. ALK-positive anaplastic T-cell lymphoma, combined small-cell/ lymphohistiocytic variant, mimicking Kikuchi-Fujimoto (subacute/histiocytic necrotizing) lymphadenitis. In: Cooke RA, editor. Surgical pathology: a Singapore-Malaysia experience. Slide seminar, XXV Congress of the International Academy of Pathology; 2004 Oct 11–16; Brisbane. Brisbane: Knowledge Books and Software, 2004: 79–89
Beaubier NT, Hart AP, Bartolo C, et al. Comparison of capillary electrophoresis and polyacrylamide gel electrophoresis for the evaluation of T and B cell clonality by polymerase chain reaction. Diagn Mol Pathol 2000; 9(3): 121–31
Sprouse J, Werling R, Hanke D, et al. T-cell clonality determination using polymerase chain reaction (PCR) amplification of the T-cell receptor gammachain gene and capillary electrophoresis of fluorescently-labelled products. Am J Clin Pathol 2000; 113: 838–50
Simon M, Kind P, Kaudewitz P, et al. Automated high-resolution polymerase chain reaction fragment analysis. A method for detecting T-cell receptor gammachain gene rearrangements in lymphoproliferative diseases. Am J Pathol 1998; 152(1): 29–33
Benharroch D, Meguerian-Bedoyan Z, Lamant L, et al. ALK-positive lymphoma: a single disease with a broad spectrum of morphology. Blood 1998 Mar 15; 91(6): 2076–84
Tan LHC, Chen CS, Mow BMF, et al. Medullary myeloid disorders in young patients negate large-cell lymphoma and favor extramedullary myeloblastic tumor/granulocytic sarcoma. Proceedings of the 4th Asia-Pacific International Academy of Pathology Congress; 2005 Aug 23–26; Beijing. Bologna: Medimond International Proceedings, 2005: 119–23
Sanchez I, San Miguel JF, Corral J, et al. Gene rearrangement in acute non-lymphoblastic leukaemia: correlation with morphological and immunophenotypic characteristics of blast cells. Br J Haematol 1995 Jan; 89(1): 104–9
Paietta E, Van Ness B, Bennett J, et al. Lymphoid lineage-associated features in acute myeloid leukaemia: phenotypic and genotypic correlations. Br J Haematol 1992 Oct; 82(2): 324–31
Rudiger T, Jaffe ES, Delsol G, et al. Workshop report on Hodgkin’s disease and related diseases (‘grey zone’ lymphoma). Ann Oncol 1998; 9Suppl. 5: S31–8
Mohrmann RL, Arber DA. CD20-positive peripheral T-cell lymphoma: report of a case after nodular sclerosis Hodgkin’ s disease and review of the literature. Mod Pathol 2000 Nov; 13(11): 1244–52
Hodges E, Krishna MT, Pickard C, et al. Diagnostic role of tests for T cell receptor (TCR) genes. J Clin Pathol 2003; 56: 1–11
Ye MQ, Suriawinata A, Black C, et al. Primary hepatic marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue type in a patient with primary biliary cirrhosis. Arch Pathol Lab Med 2000 Apr; 124(4): 604–8
Prabhu RM, Medeiros LJ, Kumar D, et al. Primary hepatic low-grade B-cell lymphoma of mucosa-associated lymphoid tissue (MALT) associated with primary biliary cirrhosis. Mod Pathol 1998 Apr; 11(4): 404–10
Stroehmann A, Dorner T, Lukowsky A, et al. Cutaneous T cell lymphoma in a patient with primary biliary cirrhosis and secondary Sjogren’s syndrome. J Rheumatol 2002 Jun; 29(6): 1326–9
Childs CC, Parham DM, Berard CW. Infectious mononucleosis. The spectrum of morphologic changes simulating lymphoma in lymph nodes and tonsils. Am J Surg Pathol 1987 Feb; 11(2): 122–32
Tindle BH, Parker JW, Lukes RJ. “Reed-Sternberg cells” in infectious mononucleosis? Am J Clin Pathol 1972 Dec; 58(6): 607–17
Lukes RJ. Criteria for involvement of lymph node, bone marrow, spleen, and liver in Hodgkin’s disease. Cancer Res 1971 Nov; 31(11): 1755–67
Kadin ME, Glatstein E, Dorfman RF. Clinicopathologic studies of 117 untreated patients subjected to laparotomy for the staging of Hodgkin’s disease. Cancer 1971 Jun; 27(6): 1277–94
Van Parys G, de Wolf-Peeters C, van den Oord JJ, et al. Lymph node architecture in Hodgkin’s disease: evidence for the role of the composite nodule in nodular sclerosing Hodgkin’s disease. Hematol Oncol 1987 Apr–Jun; 5(2): 147–54
Anagnostopoulos I, Hansmann ML, Franssila K, et al. European Task Force on Lymphoma project on lymphocyte predominance Hodgkin disease: histologic and immunohistologic analysis of submitted cases reveals 2 types of Hodgkin disease with a nodular growth pattern and abundant lymphocytes. Blood 2000 Sep 1; 96(5): 1889–99
Cabone A, Gloghini A, Gaidano G, et al. Expression status of BCL-6 and syndecan-1 identifies distinct histogenetic subtypes of Hodgkin’s disease. Blood 1998 Oct 1; 92(7): 2220–8
Carbone A, Gloghini A, Aldinucci D, et al. Expression pattern of MUM1/IRF4 in the spectrum of pathology of Hodgkin’s disease. Br J Haematol 2002 May; 117(2): 366–72
Skinnider BF, Mak TW. The role of cytokines in classical Hodgkin lymphoma. Blood 2002 Jun 15; 99(12): 4283–97
Tsang WY, Chan JK, Sing C. The nature of Reed-Sternberg-like cells in chronic lymphocytic leukemia. Am J Clin Pathol 1993 Mar; 99(3): 317–23
Roers A, Montesinos-Rongen M, Hansmann ML, et al. Amplification of TCRbeta gene rearrangements from micromanipulated single cells: T cells rosetting around Hodgkin and Reed-Sternberg cells in Hodgkin’s disease are polyclonal. Eur J Immunol 1998 Aug; 28(8): 2424–31
Poppema S, Potters M, Visser L, et al. Immune escape mechanisms in Hodgkin’s disease. Ann Oncol 1998; 9Suppl. 5: S21–4
Cazals-Hatem D, Andre M, Mounier N, et al. Pathologic and clinical features of 77 Hodgkin’s lymphoma patients treated in a lymphoma protocol (LNH87). Am J Surg Pathol 2001 Mar; 25(3): 297–306
Brousset P, Rochaix P, Chittal S, et al. High incidence of Epstein-Barr virus detection in Hodgkin’s disease and absence of detection in anaplastic large-cell lymphoma in children. Histopathology 1993 Aug; 23(2): 189–91
Calame C, Lin K-I, Tunyaplin C. Regulatory mechanisms that determine the development and function of plasma cells. Annu Rev Immunol 2003; 21: 205–30
Dong HY, Scadden DT, de Levai L, et al. Plasmablastic lymphoma in HIV-positive patients: an aggressive Epstein-Barr virus-associated extramedullary plasmacytic neoplasm. Am J Surg Pathol 2005 Dec; 29(12): 1633–41
Vega F, Chang CC, Medeiros LJ, et al. Plasmablastic lymphomas and plasmablastic plasma cell myelomas have nearly identical immunophenotypic profiles. Mod Pathol 2005 Jun; 18(6): 806–15
Colomo L, Loong F, Rives S, et al. Diffuse large B-cell lymphomas with plasmablastic differentiation represent a heterogeneous group of disease entities. Am J Surg Pathol 2004 Jun; 28(6): 736–47
Dawson-Saunders B, Trapp RG. Basic and clinical biostatistics. London: Prentice-Hall, 1994: 143–61
Seow A, Koh WP, Chia KS, et al. Trends in cancer incidence in Singapore 1968-2002. Report No. 6. Singapore: Singapore Cancer Registry, 2004
Acknowledgments
We thank Dr Puay-Hoon Tan (Singapore General Hospital [SGH]) for her contributions of Cases 5 and 6 as consultations; Dr Danilo Giron (Tan Tock Seng Hospital, Singapore) for contributing Case 10; Dr Amy Chadburn and Dr Elizabeth Hyjek (Weill-Cornell Medical College, New York-Presbyterian Hospital, New York, NY, USA) for sharing Case 14; Dr Norman Chan (Tawam Hospital, Al Ain, Abu Dhabi, UAE) for contributing Case 23; Hui-Qin Lim and Shoa-Nian Choo (National University of Singapore [NUS]), as well as Jane Tan, Maryam Hazly Hilmy and Mei-Jiuan Chng (SGH) for performing the immunohistochemical stains; Adrian Lee (SGH) for performing the molecular work on cases in SGH; Tee-Chok Tan (NUS) for assistance in photomicrography; Jean Chen (National University Hospital [NUH]) for the karyotyping of Case 25; and Wendy Ang (NUH) for configuring the tables in this article.
This work was partly funded by a grant from the Health Services Development Program of the Ministry of Health, Singapore, and partly funded by a grant (no. DCR/P30/06) from the Department of Clinical Research, SGH. The authors have no conflicts of interest that are directly relevant to the content of this study.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Rights and permissions
About this article
Cite this article
Tan, L.H.C., Chiu, LL. & Koay, E.S.C. Diagnostic Impact of Molecular Lineage Analysis on Paraffin-Embedded Tissue in Hematolymphoid Neoplasia Reclassified by Current WHO Criteria. Mol Diag Ther 11, 29–53 (2007). https://doi.org/10.1007/BF03256221
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03256221