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Journal of the Iranian Chemical Society

, Volume 6, Issue 1, pp 145–152 | Cite as

DNA-Binding and cytotoxicity of the cobalt(III) ethylenediamine pyrazole complex [Co(en)2(pyz)2]3+

  • P. Nagababu
  • J. Naveena lavanya latha
  • M. Rajesh
  • S. Satyanarayana
Article

Abstract

The complex of cobalt(III) ethylenediamine was synthesized, isolated and characterized by UV-Vis, IR, and 1H NMR spectral methods. The binding of the complex with calf thymus DNA was investigated by absorption and emission spectroscopy, viscosity measurements, DNA melting and DNA photocleavage. The spectroscopic studies together with the viscosity measurements and DNA melting studies indicated that the complex binds to calf thymus DNA in a nonintercalative mode. This complex is found to promote photocleavage of the DNA plasmid pBR322 and shows a cytotoxic effect against CHO cells.

Keywords

DNA-binding Cobalt(III) complexes Bioinorganic chemistry Cytotoxic activity 

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References

  1. [1]
    L.-N. Ji, X.-H. Zou, J.-G. Liu, Coord. Chem. Rev. 216- 217 (2001) 513.Google Scholar
  2. [2]
    N.A.P. Kane-Maguire, J.F. Wheeler, Coord. Chem. Rev. 211 (2001) 145.Google Scholar
  3. [3]
    K.E. Erkkila, D.T. Odom, J.K. Barton, Chem. Rev. 99 (1999) 2777.Google Scholar
  4. [4]
    Y. Xiong, L.-N. Ji, Coord. Chem. Rev. 185/186 (1999) 711.Google Scholar
  5. [5]
    S.O. Kelly, J.K. Barton, in: A. Sigel, H. Sigel (Eds.), Metal Ions in Biological Systems, Vol. 39, Marcel Dekker, New York, 1998, pp. 211–249.Google Scholar
  6. [6]
    B. Norden, P. Lincoln, B. Akerman, E. Tuite, in: A. Sigel, H. Sigel (Eds.), Metal Ions in Biological Systems, Vol. 33, Marcel Dekker, New York, 1996, pp. 177–252.Google Scholar
  7. [7]
    A.K. Mesmaeker, J.P. Lecomte, J.M. Kelly, in: J. Mattay (Ed.), Topics in Current Chemistry, Vol. 177, 1996, pp. 25–76.Google Scholar
  8. [8]
    H.H. Thorp, Adv. Inorg. Chem. 43 (1995) 127.Google Scholar
  9. [9]
    D.S. Sigman, A. Mazumder, D.M. Perrin, Chem. Rev. 93 (1993) 2295.Google Scholar
  10. [10]
    P. Nagababu, J.N. Lavanyalatha, S. Satyanarayana, Chem. Biodivers. 3 (2006) 1219.Google Scholar
  11. [11]
    J.K. Barton, Science 233 (1986) 727.Google Scholar
  12. [12]
    C.S. Chow, J.K. Barton, J. Am. Chem. Soc. 112 (1990) 2839.Google Scholar
  13. [13]
    X. Qu, J.B. Chaires, J. Am. Chem. Soc. 123 (2001) 1.Google Scholar
  14. [14]
    P. Nagababu, S. Satyanarayana, Polyhedron 26 (2007) 1686–1692.Google Scholar
  15. [15]
    Y. Xiong, L.N. Ji, Coord. Chem. Rev. 185 (1996) 711.Google Scholar
  16. [16]
    J.K. Barton, J. Biomol. Struct. Dyn. 1 (1983) 621.Google Scholar
  17. [17]
    J.K. Barton, A.L. Raphael, J. Am. Chem. Soc. 106 (1984) 2466.Google Scholar
  18. [18]
    A. Barve, S. Ghosh, A. Kumbhar, A. Kumbhar, V. Puranik, Tans. Metal Chem. 30 (2005) 312.Google Scholar
  19. [19]
    J. Marmur, J. Mol. Biol. 3 (1961) 208.Google Scholar
  20. [20]
    M.F. Reichmann, S.A. Rice, C.A. Thomas, P. Doty, J. Am. Chem. Soc. 76 (1954) 3047.Google Scholar
  21. [21]
    W.C. Fernelius, Inorg. Synth. 2 (1946) 222.Google Scholar
  22. [22]
    A.C. Barve, S. Ghosh, A.A.A. Avinash Kumbhar, A.S. Kumbhar, Trans. Metal Chem. 30 (2005) 312.Google Scholar
  23. [23]
    P. Nagababu, J. Naveena Lavanya Latha, P. Pallavi, S. Harish, S. Satyanarayana, Can. J. Microbiol. 52 (2006) 1247.Google Scholar
  24. [24]
    P. Nagababu, D. Aravind Kumar, K. Laxma Reddy, K. Ashwini Kumar, Md.B. Mustafa, M. Shlipa, S. Satyanarayana, Metal Based Drugs 2008, Articale ID 75084, p. 8.Google Scholar
  25. [25]
    J.B. Chaires, N. Dattaguota, D.M. Crothers, Biochem. 21 (1982) 3940.Google Scholar
  26. [26]
    S. Satyanarayana, J.C. Dabrowik, J.B. Chaires, Biochem. 32 (1993) 2573.Google Scholar
  27. [27]
    P.A. Sharp, B. Sugden, J. Sambrook, Biochem. 12 (1973) 3055.Google Scholar
  28. [28]
    S.T. Rapid, J. Immunol. Methods 65 (1983) 55.Google Scholar
  29. [29]
    K. Nazeeruddin, S.M. Zakeeruddin, R. Humphry- Baker, S.I. Gorelsky, A.B.P. Lever, M. Gratzel, Coord. Chem. Rev. 208 (2000) 213.Google Scholar
  30. [30]
    M. Wolfe, G.H. Shimer, T. Meehan, Biochemistry 26 (1987) 6392.Google Scholar
  31. [31]
    S. Satyanarayana, J.C. Dabrowiak, J.B. Chaires, Biochem. 31 (1992) 9319.Google Scholar
  32. [32]
    J.M. Kelly, A.B. Tossi, D.J. McConnell, C. OhUigin, Nucleic Acids Res. 13 (1985) 6017.Google Scholar
  33. [33]
    E. Tselepi-Kalouli, N. Katsaros, J. Inorg. Biochem. 37 (1989) 271.Google Scholar
  34. [34]
    R.B. Lopez, B.L. Loen, T. Boussie, T.J. Meyer, Tetrahedron Lett. 37 (1996) 5437.Google Scholar
  35. [35]
    D.S. Sigman, D.R. Graham, L. E. Marshall, K.A. Reich, J. Am. Chem. Soc. 102 (1980) 5490.Google Scholar
  36. [36]
    J.K. Barton, A.L. Raphael, J. Am. Chem. Soc. 106 (1984) 2466.Google Scholar

Copyright information

© Iranian Chemical Society 2009

Authors and Affiliations

  • P. Nagababu
    • 1
  • J. Naveena lavanya latha
    • 1
  • M. Rajesh
    • 2
  • S. Satyanarayana
    • 1
  1. 1.Department of ChemistryOsmania UniversityHyderabadIndia
  2. 2.Department of BiochemistryOsmania UniversityHyderabadIndia

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