Advertisement

Journal of the Iranian Chemical Society

, Volume 7, Issue 2, pp 516–520 | Cite as

High performance liquid chromatographic determination of sodium benzoate, methylparaben and propylparaben as preservative components in nystatin suspensions

  • A. Abdollahpour
  • M. Forouhi
  • M. Shamsipur
  • Y. Yamini
Article

Abstract

A simple and sensitive method was developed for the analysis of preservatives sodium benzoate, methylparaben and propylparaben in nystatin suspensions by reversed-phase high performance liquid chromatography (HPLC), equipped with a C18 column and PDA detector. The mobile phase was a mixture of acetonitrile and acetate buffer of pH 4.4 (35:65 v/v). Under the optimized experimental conditions, separation of the preservatives was achieved in less than 20 min. The limits of quantifications (LOQs) and the linear dynamic ranges (LDRs) of sodium benzoate, methylparaben and propylparaben were 0.3 and 50–1000 μg Ml−1, 0.5 and 50–600 μg ml−1 and 0.3 and 50–900 μg ml−1, respectively; the respective precisions (%RSD) at 500 μg ml−1 level were 0.72%, 0.73% and 0.51% (n = 6). The average recoveries of sodium benzoate, methylparaben and propylparaben for spiked nystatin samples were obtained as 98%, 97% and 98%, respectively.

Keywords

Preservative Determination Nystatin Reversed phase HPLC 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    E.L. Hazen, R. Brown, Science 423 (1950) 112.Google Scholar
  2. [2]
    USP DI 2004, 24th ed., Drug Information for the Health Care Professional (2004).Google Scholar
  3. [3]
    F.D. Croo, J.D. Schutter, W. Van den Bossche, P.D. Moerloose, Chromatographia 260 (1984) 18.Google Scholar
  4. [4]
    H.J. Lin, Y.M. Choong, J. Food Drug. Anal. 291 (1999) 7.Google Scholar
  5. [5]
    Y.J. Heo, K.J. Lee, J. Pharm. Biomed. Anal. 1371 (1998) 17.Google Scholar
  6. [6]
    S.P. Wang, C.L. Chang, Anal. Chim. Acta 85 (1998) 377.Google Scholar
  7. [7]
    Y.H. Lin, S.S. Chou, F. Sheu, Y.T. Shgu, J. Chromatogr. Sci. 345 (2000) 38.Google Scholar
  8. [8]
    D. Ivanovic, M. Medenica, E.N. Guernet, M. Guernet, Chromatographia 652 (1995) 40.Google Scholar
  9. [9]
    G. Burini, J. Chromatogr. A 213 (1994) 664.Google Scholar
  10. [10]
    A.H. Kibbe, Handbook of Pharmaceutical Excipients, 3th ed., 2000, pp. 340, 450, 471.Google Scholar
  11. [11]
    R. Hajkova, P. Solich, M. Pospisilova, J. Sicha, Anal. Chim. Acta 91 (2002) 467.Google Scholar
  12. [12]
    J.A. Arancibia, M.A. Boldrini, G.M. Escandar, Talanta 261 (2000) 52.Google Scholar
  13. [13]
    The European Pharmacopoeia, 4th ed. (Suppl. 4.3), Council of Europe, Strasbourg, France, 2003, p. 3065.Google Scholar
  14. [14]
    USP 30, United States Pharmacopoeial Convention Inc., 2007, p. 136.Google Scholar

Copyright information

© Iranian Chemical Society 2010

Authors and Affiliations

  • A. Abdollahpour
    • 1
  • M. Forouhi
    • 2
  • M. Shamsipur
    • 3
  • Y. Yamini
    • 1
  1. 1.Department of ChemistryTarbiat Modares UniversityTehranIran
  2. 2.Jaber Ebne Hayyan Pharmaceutical IndustryTehranIran
  3. 3.Department of ChemistryRazi UniversityKermanshahIran

Personalised recommendations