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Journal of the Iranian Chemical Society

, Volume 5, Issue 1, pp 115–121 | Cite as

Application of arsenazo III for the polarographic detection of proteins

  • W. Sun
  • N. Zhao
  • B. Xu
  • M. N. Wang
  • K. Jiao
Article

Abstract

In this paper, a diazo dye of arsenazo III (AAIII) was selected as a new electrochemical probe for the determination of proteins. In Britton-Robinson (B-R) buffer solution of pH 2.4, AAIII had a sensitive second order derivative linear sweep voltammetric reductive peak at −0.39 V (vs. SCE). After the addition of human serum albumin (HSA) into AAIII solution, an interaction was taken place in the mixed solution and a biosupramolecular complex was formed, which resulted in the decreased reductive peak currents of AAIII. Based on the observed decrease in peak current, a sensitive electrochemical method was proposed for the determination of different proteins such as HSA, bovine serum albumin (BSA) and bovine hemoglobin (BHb). The optimal conditions for the interaction and the interfering effects of coexisting substances on the detection were investigated. The proposed method was successfully applied to the determination of HSA in synthetic samples with the recoveries in the range of 99.13–100.50%. The stoichiometry of HSA-AAIII biocomplex was calculated by voltammetric data with a binding number of 2 and a binding constant of 7.53 × 109.

Keywords

Arsenazo III Human serum albumin Linear sweep voltammetry Interaction Protein assay 

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Copyright information

© Iranian Chemical Society 2008

Authors and Affiliations

  1. 1.College of Chemistry and Molecular EngineeringQingdao University of Science and TechnologyQingdaoP.R. China

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