Micro-patterned polydiacetylene vesicle chips for detecting protein-protein interactions
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In conclusion, micro-patterned PDA vesicles were proved as a biosensor to detect a protein-protein interaction. The vesicles were conjugated with primary antibodies and immobilized on glass slides as an array form. The fluorescence level of the PDA vesicles was changed by proteinprotein interactions between primary and secondary antibodies. This experiment proves that the micro-patterned PDA vesicles are useful to detect protein level interactions and can serve as a novel substrate to develop a protein chip.
KeywordsFluorescence Level Protein Chip Green Filter Ondary Antibody Thin Lipid Film
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