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Macromolecular Research

, Volume 11, Issue 6, pp 488–494 | Cite as

Behavior of hepatocytes inoculated in gelatin-immobilized polyurethane foam

  • Kyung-Su Yang
  • Xinglin Guo
  • Wan Meng
  • Jae-Yong Hyun
  • Inn-Kyu Kang
  • Yang Il Kim
Article

Abstract

We have fabricated gelatin-immobilized polyurethane foams (PUFG) by dipping polyurethane foam (PUF) in an aqueous solution containing gelatin and by subsequent reaction with glutaraldehyde after freeze-drying. Gelatin aqueous solutions of different concentrations were used as the dipping solutions to control the amount of immobilized gelatin. The average pore size of PUF decreased with an increase in gelatin concentration. It was found from the hepatocyte adhesion experiment that the amount of hepatocytes seeded on PUFG1, prepared by using a 1% aqueous gelatin solution, was higher than that on other PUFGs. The hepatocytes inoculated in PUFG1 were slightly aggregated as the incubation time increased. The cells inoculated in PUFG1 showed higher ammonia removal ability than those monolayer-cultured on a gelatin-immobilized polystyrene dish (PSG) after 1 and 4 days of incubation time. The inoculated cells exhibited higher albumin secretion relative to monolayer-cultured hepatocytes on PSG. Albumin secretion by hepatocytes seeded on PUFG1 was increased by the presence of serum and was further increased by both the presence of serum and cytokines. The results obtained from a 3-(3,4-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay indicated that PUFG can provide a better microenvironment for hepatocyte culture along with nutrition and metabolite transfer through the high porosity of PUF.

Keywords

gelatin polyurethane foam hepatocyte albumin secretion 

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References

  1. (1).
    K. Nakazawa, H. Ijima, J. Fukuda, R. Sakiyama, Y. Yamashita, M. Shimada, K. Shirabe, E. Tsujita, K. Sugimachi, and K. Funatsu,Int. J. Artif. Organs,25, 51 (2002).Google Scholar
  2. (2).
    J. Fukuda, R. Sakiyama, K. Nakazawa, H. Ijima, Y. Yamashita, M. Shimada, K. Shirabe, E. Tsujita, K. Sugimachi, and K. Funatsu,Int. J. Artif. Organs,24, 799 (2001).Google Scholar
  3. (3).
    K. Funatsu, H. Ijima, K. Nakazawa, Y. Yamashita, M. Shimada, and K. Sugimachi,Artif. Organs,25, 194 (2001).CrossRefGoogle Scholar
  4. (4).
    Y. Yamashita, M. Shimada, E. Tsujita, T. Rikimaru, H. Ijima, K. Nakazawa, R. Sakiyama, J. Fukuda, and K. Funatsu,Int. J. Artif. Organs,24, 34 (2001).Google Scholar
  5. (5).
    Y. Yamashita, M. Shimada, E. Tsujita, S. Tanaka, H. Ijima, K. Nakazawa, R. Sakiyama, J. Fukuda, T. Ueda, and K. Funatsu,Cell Transp.,10, 717 (2001).Google Scholar
  6. (6).
    H. Kurosawa, K. Yasumoto, T. Kimura, and Y. Amano,Biotech. Bioeng.,70, 160 (2000).CrossRefGoogle Scholar
  7. (7).
    H. Ijima, K. Nakazawa, S. Koyama, M. Kaneko, T. Matsushita, T. Gion, K. Shirabe, M. Shimada, K. Takenaka, K. Sugimachi, and K. Funatsu,Int. J. Artif. Organs,23, 446 (2000).Google Scholar
  8. (8).
    H. Ijima, K. Nakazawa, S. Koyama, M. Kaneko, T. Matsushita, T. Gion, K. Shirabe, M. Shimada, K. Takenaka, K. Sugimachi, and K. Funatsu,Int. J. Artif. Organs,23, 389 (2000).Google Scholar
  9. (9).
    Y. Fujii, K. Nakazawa, and K. Funatsu,J. Biomater. Sci., Polymer Edn.,11, 731 (2000).CrossRefGoogle Scholar
  10. (10).
    H. Ijima, K. Nakazawa, H. Mizumoto, T. Matsushita, and K. Funatsu,J. Biomater. Sci., Polymer Edn.,9, 765 (1998).CrossRefGoogle Scholar
  11. (11).
    T. Matsushita, H. Ijima, N. Koide, and K. Funatsu,Appl. Microbio. Biotech.,36, 324 (1991).Google Scholar
  12. (12).
    Y. Ikada and T. Okano,Tissue Engineering for Therapeutic Use 3, Elsevier Scientific Publishing Co., Amsterdam, 1999.Google Scholar
  13. (13).
    M. Kaneko, J. Fukuda, H. Ijima, K. Nakazawa, T. Gion, M. Shimada, K. Shirabe, K. Takenaka, K. Sugimachi, and K. Funatsu.Mater. Sci. Eng.,C6, 245 (1998).CrossRefGoogle Scholar
  14. (14).
    H. Ijima, T. Matsushita, K. Nakazawa, Y. Fujii, and K. Funatsu,Tissue. Eng.,4, 213 (1998).CrossRefGoogle Scholar
  15. (15).
    T. Gion, M. Shimada, M. Shimada, K. Shirabe, K. Nakazawa, H. Ijima, T. Matsushita, K. Funatsu, and K. Sugimachi,J. Surg. Res.,82, 131 (1999).CrossRefGoogle Scholar
  16. (16).
    Y. Sato, T. Ochiya, Y. Yasuda, and K. Matsubara,Hepatology,19, 1023 (1994).CrossRefGoogle Scholar
  17. (17).
    J. A. Rowley, G. Madlabayan, and D. J. Mooney,Biomaterials,20, 45 (1999).CrossRefGoogle Scholar
  18. (18).
    S. T. Lopina, G. Wu, E. W. Merrill, and L. G. Cima,Biomaterials,17, 559 (1996).CrossRefGoogle Scholar
  19. (19).
    T. Matsuda and M. J. Moghaddam,J. Polym. Sci. Polym. Chem.,31, 1589 (1993).CrossRefGoogle Scholar
  20. (20).
    Y. C. Lee and R. T. Lee,Neoglycoconjugates: Preparation and Application, Academic Press, San Diego, 1994.Google Scholar
  21. (21).
    B. J. Nathan and P. J. Evans,Cryobiology,40, 176 (2000).CrossRefGoogle Scholar
  22. (22).
    P. J. Evans,Cell. Biol. Intern.,19, 855 (1995).CrossRefGoogle Scholar
  23. (23).
    S. Zmora, R. Glicklis, and S. Cohen,Biomaterials,23, 4087 (2002).CrossRefGoogle Scholar
  24. (24).
    J. Yang, M. Goto, H. Ise, C. S. Cho, and T. Akaike,Biomaterials,23, 471 (2002).CrossRefGoogle Scholar
  25. (25).
    Y. Ikada and T. Okano,Tissue Engineering for Therapeutic Use 3, Elsevier Scientific Publishing Co., Amsterdam, 1999.Google Scholar
  26. (26).
    N. Ohshima, K. Yanagi, and H. Miyoshi,Artif. Organs,21, 1169 (1997).CrossRefGoogle Scholar
  27. (27).
    K. Yanagi, S. Mizuno, and N. Ohshima,Trans. Am. Soc. Artif. Intern. Organs,36, 727 (1990).Google Scholar
  28. (28).
    K. Yanagi, H. Miyoshi, H. Fukuda, and N. Ohshima,Appl. Microbiol. Biotech.,37, 316 (1992).CrossRefGoogle Scholar
  29. (29).
    S. J. Lee, Y. M. Lee, G. Khang, I. Y. Kim, B. Lee, and H. B. Lee,Macromol. Res.,10, 150 (2002).CrossRefGoogle Scholar
  30. (30).
    G. Khang, C. S. Park, J. M. Lee, S. J. Lee, Y. M. Lee, M. K. Choi, I. Lee, and H. B. Lee,Korea Polym. J.,9, 267, (2001).Google Scholar
  31. (31).
    P. O. Seglen,Methods Cell Biol.,13, 23 (1976).Google Scholar
  32. (32).
    A. T. Gutsche, H. L. Zurlo, J. J. Yager, and K. W. Leong,Biomaterials,17, 387 (1996).CrossRefGoogle Scholar
  33. (33).
    J. Yang, T. W. Chung, M. Nagaoka, M. Goto, C. S. Cho, and T. Akaike,Biotechnol. Lett.,23, 1385 (2001).CrossRefGoogle Scholar

Copyright information

© Polymer Society of Korea 2003

Authors and Affiliations

  • Kyung-Su Yang
    • 1
  • Xinglin Guo
    • 1
  • Wan Meng
    • 1
  • Jae-Yong Hyun
    • 1
  • Inn-Kyu Kang
    • 1
  • Yang Il Kim
    • 2
  1. 1.Department of Polymer ScienceKyungpook National UniversityDaeguKorea
  2. 2.School of MedicineKyungpook National UniversityDaeguKorea

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