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RGD Island Spacing Controls Phenotype of Primary Human Fibroblasts Adhered to Ligand-Organized Hydrogels

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Conclusions

We demonstrated that the nanoscale organization of RGD peptides in alginate gels, designed as a synthetic ECM, regulated the adhesion and proliferation of primary human fibroblasts. Cell spreading and growth were strongly correlated with the distance between islands of the peptides, irrespective of the same bulk RGD density in the gels. The approach of controlling the distribution and spacing of adhesion ligands at the nanometer level may be critical in the design and function of synthetic ECMs for tissue engineering applications.

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Authors and Affiliations

  1. Department of Bioengineering, Hanyang University, 133-791, Seoul, Korea

    Min-Su Bae & Kuen Yong Lee

  2. Craniomaxillofacial Reconstructive Science Major, School of Dentistry, Seoul National University, 110-749, Seoul, Korea

    Yoon Jeong Park

  3. Division of Engineering and Applied Sciences, Harvard University, MA 02138, Cambridge, USA

    David J. Mooney

Authors
  1. Min-Su Bae
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  2. Kuen Yong Lee
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  3. Yoon Jeong Park
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  4. David J. Mooney
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Correspondence to Kuen Yong Lee.

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Bae, MS., Lee, K.Y., Park, Y.J. et al. RGD Island Spacing Controls Phenotype of Primary Human Fibroblasts Adhered to Ligand-Organized Hydrogels. Macromol. Res. 15, 469–472 (2007). https://doi.org/10.1007/BF03218816

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  • DOI: https://doi.org/10.1007/BF03218816

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