Advertisement

Macromolecular Research

, Volume 15, Issue 5, pp 469–472 | Cite as

RGD Island Spacing Controls Phenotype of Primary Human Fibroblasts Adhered to Ligand-Organized Hydrogels

  • Min-Su Bae
  • Kuen Yong Lee
  • Yoon Jeong Park
  • David J. Mooney
Article

Conclusions

We demonstrated that the nanoscale organization of RGD peptides in alginate gels, designed as a synthetic ECM, regulated the adhesion and proliferation of primary human fibroblasts. Cell spreading and growth were strongly correlated with the distance between islands of the peptides, irrespective of the same bulk RGD density in the gels. The approach of controlling the distribution and spacing of adhesion ligands at the nanometer level may be critical in the design and function of synthetic ECMs for tissue engineering applications.

Keywords

Alginate Primary Fibroblast Nanometer Level Primary Human Fibroblast Adhesion Ligand 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. (1).
    R. Langer and J. P. Vacanti,Science,260, 920 (1993).CrossRefGoogle Scholar
  2. (2).
    K. Y. Lee and D. J. Mooney,Chem. Rev.,101, 1869 (2001).CrossRefGoogle Scholar
  3. (3).
    K. Y. Lee,Macromol. Res.,13, 277 (2005).CrossRefGoogle Scholar
  4. (4).
    M. E. Duggan and J. H. Hutchinson,Exp. Opin. Ther. Pat.,10, 1367 (2000).CrossRefGoogle Scholar
  5. (5).
    E. Ruoslahti,Ann. Rev. Cell Dev. Biol.,12, 697 (1996).CrossRefGoogle Scholar
  6. (6).
    C. A. Buck and A. F. Horwitz,Ann. Rev. Cell Biol.,3, 179 (1987).CrossRefGoogle Scholar
  7. (7).
    J. A. Hubbell,Bio-Technol.,13, 565 (1995).Google Scholar
  8. (8).
    E. Alsberg, K. W. Anderson, A. Albeiruti, J. A. Rowley, and D. J. Mooney,Proc. Natl. Acad. Sci. USA,99, 12025 (2002).CrossRefGoogle Scholar
  9. (9).
    C. S. Chen, M. Mrksich, S. Huang, G. M. Whitesides, and D. E. Ingber,Science,276, 1425 (1997).CrossRefGoogle Scholar
  10. (10).
    J. S. Tjia and P. V. Moghe,Tissue Eng.,8, 247 (2002).CrossRefGoogle Scholar
  11. (11).
    S. P. Massia and J. A. Hubbell,J. Cell Biol.,114, 1089 (1991).CrossRefGoogle Scholar
  12. (12).
    G. Maheshwari, G. Brown, D. A. Lauffenburger, A. Wells, and L. G. Griffith,J. Cell. Sci.,113, 1677 (2000).Google Scholar
  13. (13).
    K. Y. Lee, E. Alsberg, S. Hsiong, W. Comisar, J. Linderman, R. Ziff, and D. Mooney,Nano Letters,4, 1501 (2004).CrossRefGoogle Scholar
  14. (14).
    K. S. Park, C. M. Jin, S. H. Kim, J. M. Rhee, G. Khang, C. W. Han, Y. S. Yang, M. S. Kim, and H. B. Lee,Macromol. Res.,13, 285 (2005).CrossRefGoogle Scholar
  15. (15).
    A. D. Augst, H. J. Kong, and D. J. Mooney,Macromol. Biosci.,6, 623 (2006).CrossRefGoogle Scholar
  16. (16).
    J. A. Rowley, G. Madlambayan, and D. J. Mooney,Biomaterials,20, 45 (1999).CrossRefGoogle Scholar
  17. (17).
    S. I. Han, B. S. Kim, S.W. Kang, H. Shirai, and S. S. Im,Biomateials,24, 3453 (2003).CrossRefGoogle Scholar
  18. (18).
    B. K. Mann, A. T. Tsai, T. Scott-Burden, and J. L. West,Biomaterials,20, 2281 (1999).CrossRefGoogle Scholar
  19. (19).
    E. Alsberg, K. W. Anderson, A. Albeiruti, R. T. Franceschi, and D. J. Mooney,J. Dental Res.,80, 2025 (2001).CrossRefGoogle Scholar
  20. (20).
    I. I. Singer, D. W. Kawka, S. Scott, R. A. Mumford, and M. W. Lark,J. Cell Biol.,104, 573 (1987).CrossRefGoogle Scholar
  21. (21).
    Y. N. Danilov and R. L. Juliano,Exp. Cell Res.,182, 186 (1989).CrossRefGoogle Scholar
  22. (22).
    B. K. Brandley and R. L. Schnaar,Anal. Biochem.,172, 270 (1988).CrossRefGoogle Scholar

Copyright information

© The Polymer Society of Korea and Springer 2007

Authors and Affiliations

  • Min-Su Bae
    • 1
  • Kuen Yong Lee
    • 1
  • Yoon Jeong Park
    • 2
  • David J. Mooney
    • 3
  1. 1.Department of BioengineeringHanyang UniversitySeoulKorea
  2. 2.Craniomaxillofacial Reconstructive Science MajorSchool of Dentistry, Seoul National UniversitySeoulKorea
  3. 3.Division of Engineering and Applied SciencesHarvard UniversityCambridgeUSA

Personalised recommendations