Macromolecular Research

, Volume 17, Issue 12, pp 956–962 | Cite as

Effect of nonlamellar-prone lipids on protein encapsulation in liposomes

  • Taeho Ahn
  • Youn-Tae Chi
  • Chul-Ho Yun


We investigated the effect of two nonlamellar-prone lipids, phosphatidylethanolamine (PE) and dioleoylglycerol (DOG), on the efficiency of protein encapsulation in liposomes. When the phosphatidylcholine (PC) matrix was replaced with PE or DOG during liposome formulation, the amounts of glutathione S-transferase and bovine serum albumin entrapped in the vesicles increased with increasing PE or DOG concentration. The presence of PE and DOG synergistically affected protein entrapment. These results suggest that protein encapsulation can be enhanced by the presence of nonlamellar lipids and/or lipid-induced membrane properties.


protein encapsulation nonlamellar-prone lipids liposomes 


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  1. (1).
    O. P. Medina, Y. Zhu, and K. Kairemo,Curr. Pharm. Des.,10, 2981 (2004).CrossRefGoogle Scholar
  2. (2).
    M. Winterhalter and D. D. Lasic,Chem. Phys. Lipids,64, 35 (1993).CrossRefGoogle Scholar
  3. (3).
    F. Jr. Szoka and D. Papahadjopoulos,Annu. Rev. Biophys. Bioeng.,9, 467 (1980).CrossRefGoogle Scholar
  4. (4).
    F. Jr. Szoka and D. Papahadjopoulos,Proc. Natl. Acad. Sci. USA,75, 4194 (1978).CrossRefGoogle Scholar
  5. (5).
    D. Lichtenberg and Y. Barenholtz,Methods Biochem. Anal.,33, 337 (1988).CrossRefGoogle Scholar
  6. (6).
    C. Kirby and G. Gregoriadis,J. Microencapsul.,1, 33 (1984).CrossRefGoogle Scholar
  7. (7).
    T. Ahn, F. P. Guengerich, and C.-H. Yun,Biochemistry,37, 12860 (1998).CrossRefGoogle Scholar
  8. (8).
    S. Kölchens, V. Ramaswami, J. Birgenheier, L. Nett, and D. F. O’Brien,Chem. Phys. Lipids,65, 1 (1993).CrossRefGoogle Scholar
  9. (9).
    V. E. Vaskovsky, E. Y. Kostetsky, and I. M. Vasendin,J. Chromatogr.,114, 129 (1975).CrossRefGoogle Scholar
  10. (10).
    I. Stern and B. Shapiro,J. Clin. Pathol. (London),6, 158 (1953).CrossRefGoogle Scholar
  11. (11).
    W. H. Habig and W. B. Jakoby,Methods Enzymol.,77, 398 (1981).CrossRefGoogle Scholar
  12. (12).
    T. Ahn and C.-H. Yun,Arch. Biochem. Biophys.,369, 288 (1999).CrossRefGoogle Scholar
  13. (13).
    A.-K. Lundbäck, L. Haneskog, L. Andersson, A. Heijbel, L. Ingemarsson, and D. Birse,Life Science News,15, 10 (2003).Google Scholar
  14. (14).
    J.-P. Colletier, B. Chaize, M. Winterhalter, and D. Fournier,BMC Biotechnol.,2, 1 (2002).CrossRefGoogle Scholar
  15. (15).
    H. Ellens, J. Bentz, and F. C. Szoka,Biochemistry,25, 285 (1986).CrossRefGoogle Scholar
  16. (16).
    B. Perly, I. C. Smith, and H. C. Jarrel,Biochemistry,24, 1055 (1985).CrossRefGoogle Scholar
  17. (17).
    R. N. Lewis, D. A. Mannock, R. N. McElhaney, D. C. Turner, and S. M. Gruner,Biochemistry,28, 541 (1989).CrossRefGoogle Scholar
  18. (18).
    S. E. Gordesky, G. V.Marinetti, and R. Love,J. Membr. Biol.,20, 111 (1975).CrossRefGoogle Scholar
  19. (19).
    R. M. Epand,Biochemistry,24, 7092 (1985).CrossRefGoogle Scholar
  20. (20).
    S. Das and R. P. Rand,Biochemistry,25, 2882 (1986).CrossRefGoogle Scholar
  21. (21).
    D. K. Hincha, A. E. Oliver, and J. H. Crowe,Biochim. Biophys. Acta,1368, 150 (1998).CrossRefGoogle Scholar
  22. (22).
    R. M. Epand and R. Bottega,Biochem. Biophys. Acta,944, 144 (1988).CrossRefGoogle Scholar
  23. (23).
    R. M. Epand,Biochemistry,24, 7092 (1985).CrossRefGoogle Scholar
  24. (24).
    S. Das and R. P. Rand,Biochemistry,25, 2882 (1986).CrossRefGoogle Scholar
  25. (25).
    R. A. Schwendener,Adv. Exp. Med. Biol.,620, 117 (2007).CrossRefGoogle Scholar
  26. (26).
    S. L. Huang,Adv. Drug Deliv. Rev.,60, 1167 (2008).CrossRefGoogle Scholar
  27. (27).
    S. Skeie,Int. Dairy J.,4, 573 (1994).CrossRefGoogle Scholar
  28. (28).
    T.-L. Hwang, W.-R. Lee, S.-C. Hua, and J.-Y. Fang,J. Dermatol. Sci.,46, 11 (2007).CrossRefGoogle Scholar

Copyright information

© The Polymer Society of Korea and Springer 2009

Authors and Affiliations

  1. 1.Department of Biochemistry, College of Veterinary MedicineChonnam National UniversityGwangjuKorea
  2. 2.School of Biological Sciences and TechnologyChonnam National UniversityGwangjuKorea

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