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Temperature controllable hplc column for preparative fractionation of polymers

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Abstract

An HPLC column with a self-contained temperature control device was constructed for preparative temperature programmed interaction chromatography. Two Peltier plates were attached to a large bore column (120×22 mm i.d.) and the column temperature was controlled by PID mode feed back control. At a flow rate of 1.5 mL/min, the column temperature could be increased and decreased at a rate as high as 50 °C/min and 10 °C/min, respectively, which is much faster than using a column jacket and bath/circulator. The rapid heating and cooling rates allows a high repetition rate of chromatographic fractionation. The performance of the temperature controllable column was demonstrated successfully by the fractionation of homo-polymer precursors from diblock copolymers.

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Authors and Affiliations

  1. Department of Chemistry and Polymer Research Institute, Pohang University of Science and Technology, 790-784, Pohang, Korea

    Kyuhyun Im, Hae-woong Park, Youngtak Kim & Taihyun Chang

Authors
  1. Kyuhyun Im
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  2. Hae-woong Park
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  3. Youngtak Kim
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  4. Taihyun Chang
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Correspondence to Taihyun Chang.

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Im, K., Park, Hw., Kim, Y. et al. Temperature controllable hplc column for preparative fractionation of polymers. Macromol. Res. 16, 544–548 (2008). https://doi.org/10.1007/BF03218557

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  • DOI: https://doi.org/10.1007/BF03218557

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