Artificial dermis composed of gelatin, hyaluronic acid and (1→3),(1→6)-β-glucan
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Porous scaffolds composed of gelatin and polysaccharides such as hyaluronic acid and β-glucan were prepared by using the freeze-drying method after cross-linking with 1-ethyl-(3-3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). The scaffold had an inter-connected pore structure with the sufficient pore size for use as a support for the growth of fibroblasts. Results for the contact angle and cell attachment confirmed that high gelatin content in a mixture was suitable for cellular attachment and distribution in two- or three-dimensional fibroblast cultures. However, the addition of polysaccharides aroused the synergistic effects of morphologic and mechanical property of gelatin-based scaffolds. To prepare the artificial dermis for the wound dressing to mimic the normal human dermal skin, fibroblasts were isolated from a childs foreskin, and cultured in gelatin-based scaffolds. Anin vivo study showed that the artificial dermis containing the fibroblasts enhanced the wound healing rate and re-epithelialization of a full-thickness skin defect rather than the acellular scaffold after one week.
Keywordsgelatin β-glucan hyaluronic acid scaffold skin
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