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Macromolecular Research

, Volume 11, Issue 5, pp 368–374 | Cite as

Artificial dermis composed of gelatin, hyaluronic acid and (1→3),(1→6)-β-glucan

  • Sang Bong Lee
  • Hyun Wook Jeon
  • Young Woo Lee
  • Seong Kwan Cho
  • Young Moo Lee
  • Kang Won Song
  • Moon Hyang Park
  • Sung Hwa Hong
Article

Abstract

Porous scaffolds composed of gelatin and polysaccharides such as hyaluronic acid and β-glucan were prepared by using the freeze-drying method after cross-linking with 1-ethyl-(3-3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). The scaffold had an inter-connected pore structure with the sufficient pore size for use as a support for the growth of fibroblasts. Results for the contact angle and cell attachment confirmed that high gelatin content in a mixture was suitable for cellular attachment and distribution in two- or three-dimensional fibroblast cultures. However, the addition of polysaccharides aroused the synergistic effects of morphologic and mechanical property of gelatin-based scaffolds. To prepare the artificial dermis for the wound dressing to mimic the normal human dermal skin, fibroblasts were isolated from a childs foreskin, and cultured in gelatin-based scaffolds. Anin vivo study showed that the artificial dermis containing the fibroblasts enhanced the wound healing rate and re-epithelialization of a full-thickness skin defect rather than the acellular scaffold after one week.

Keywords

gelatin β-glucan hyaluronic acid scaffold skin 

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Copyright information

© The Polymer Society of Korea and Springer 2003

Authors and Affiliations

  • Sang Bong Lee
    • 1
  • Hyun Wook Jeon
    • 1
  • Young Woo Lee
    • 1
  • Seong Kwan Cho
    • 1
  • Young Moo Lee
    • 1
  • Kang Won Song
    • 2
  • Moon Hyang Park
    • 2
  • Sung Hwa Hong
    • 3
  1. 1.School of Chemical Engineering, College of EngineeringHanyang UniversitySeoulKorea
  2. 2.Department of Pathology, College of MedicineHanyang UniversitySeoulKorea
  3. 3.Korea FDA Biologics Evaluation DepartmentBlood Products DivisionSeoulKorea

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