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Artificial dermis composed of gelatin, hyaluronic acid and (1→3),(1→6)-β-glucan

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Abstract

Porous scaffolds composed of gelatin and polysaccharides such as hyaluronic acid and β-glucan were prepared by using the freeze-drying method after cross-linking with 1-ethyl-(3-3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). The scaffold had an inter-connected pore structure with the sufficient pore size for use as a support for the growth of fibroblasts. Results for the contact angle and cell attachment confirmed that high gelatin content in a mixture was suitable for cellular attachment and distribution in two- or three-dimensional fibroblast cultures. However, the addition of polysaccharides aroused the synergistic effects of morphologic and mechanical property of gelatin-based scaffolds. To prepare the artificial dermis for the wound dressing to mimic the normal human dermal skin, fibroblasts were isolated from a childs foreskin, and cultured in gelatin-based scaffolds. Anin vivo study showed that the artificial dermis containing the fibroblasts enhanced the wound healing rate and re-epithelialization of a full-thickness skin defect rather than the acellular scaffold after one week.

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Authors and Affiliations

  1. School of Chemical Engineering, College of Engineering, Hanyang University, 133-791, Seoul, Korea

    Sang Bong Lee, Hyun Wook Jeon, Young Woo Lee, Seong Kwan Cho & Young Moo Lee

  2. Department of Pathology, College of Medicine, Hanyang University, 133-791, Seoul, Korea

    Kang Won Song & Moon Hyang Park

  3. Korea FDA Biologics Evaluation Department, Blood Products Division, Nokburn-Dong 5, Eunpyung-Ku, 122-070, Seoul, Korea

    Sung Hwa Hong

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  1. Sang Bong Lee
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  2. Hyun Wook Jeon
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  5. Young Moo Lee
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  7. Moon Hyang Park
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  8. Sung Hwa Hong
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Lee, S.B., Jeon, H.W., Lee, Y.W. et al. Artificial dermis composed of gelatin, hyaluronic acid and (1→3),(1→6)-β-glucan. Macromol. Res. 11, 368–374 (2003). https://doi.org/10.1007/BF03218378

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  • DOI: https://doi.org/10.1007/BF03218378

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