Acetaminophen and its regioisomer, 3-acetamidophenol interfered with the growth ofEscherichia coli O157:H7
Escherichia coli O157:H7 (O157) is an enterohemorrhagic pathogenic subtype, causing diarrhea and Hemolytic-uremic syndrome (HUS). Treatment for HUS patients might be accompanied with antibiotics and antispasmodics with analgesics for abdominal pain. Antiperistaltic effect of antispasmodics, however, may increase the risk of infection of O157 through the intestine. If analgesics also have antibacteiral effect, the prevention and cure of O157 could get benefits. Akhter, T.et al. (2010) reported the inhibitory effect of ibuprofen (IBF) on the bacterial isolates from infected urinary tract. Here, we also found that acetaminophen (4-AAP) and its regioisomer, 3-acetamidophenol (3-AAP), constrained the growth of O157. Inhibitory effect was compared with IBF, since they were both COX inhibitors for their inhibitory mechanism. The experiment results showed that O157 growth was reduced in the presence of 4-AAP, 3-AAP, and IBF. Among them, the inhibitory effect of 4-AAP was less than 3-AAP and IBF in 1.8 fold at 10 mM concentrations. In addition, 4-AAP and 3-AAP inhibited the activity of endogenous peroxidase ofE. coli, as well as, horseradish peroxidase (HRP), implying its possible inhibitory mechanism. However, IBF did not inhibit peroxidase activity, suggesting the different mechanism to suppress the growth of O157 by 4-AAP and 3-AAP.
KeywordsE. coli O157:H7 Acetaminophen 3′-hydroxyacetanilide Growth rate Inhibition
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