in vitromodels, endpoints and assessment methods for the measurement of cytotoxicity
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Cells respond rapidly to toxic substances by altering the morphology, growth, behavior, and biochemical processes controlling basic cell functions.in vitrocytotoxicity analysis, an alternative to animal-based testing, is an important tool to specifically screen the chemicals and form a comprehensive and reliable toxicological data useful for predicting the toxic effects on humans. Consequently, the increasing demand of cellbased screening system led to the development of various innovativein vitromodel systems, novel endpoints, variety of cytotoxicity assays, and advanced assessment or detection methods. Although the cellbased screening has come to a very advanced level capable of providing adequate information on kinetic progression of cell death and dynamic interaction between cells and toxicants, the certain limitations of the existing model systems and methods render the results with high variability and questionable relevance. Therefore, the multiplexed assay approach based on multiple endpoints should be preferred for the evaluation of toxicity of a chemical and to estimate the actual cause and mechanism of cell death. In addition, the existingin vitro cytotoxicity studies provide the collection of toxicological data useful for the determination of acute effects of a substance. These data don’t well suit to analyze the chronic effects of a chemical. Therefore, there is a strong need to improve the existing model systems and methods which could be applicable for the measurement of the time-course of molecular changes and complex sequences of biochemical events involved in chronic toxicityin vivo.
KeywordsCytotoxicity Assay Normal Human Lung Fibroblast Cytotoxicity Analysis Anaplastic Thyroid Cancer Cell Cell Death Progression
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