Australasian Plant Disease Notes

, Volume 5, Issue 1, pp 117–119 | Cite as

First report of ‘Candidatus Phytoplasma asteris’ (16SrI) associated with little leaf of cotton and luffa in India

  • S. Kumar
  • V. Singh
  • S. Lakhanpaul


Cotton and luffa plants with little leaf symptoms were observed during February 2010 at New Delhi, India. Nested polymerase chain reaction with phytoplasma 16S rDNA-specific primers efficiently detected the pathogen in the diseased plants. Sequence comparisons showed homology with the members of ‘Candidatus Phytoplasma asteris’, group 16SrI. Phylogenetic analysis also assigned the cotton and luffa little leaf phytoplasma to the 16SrI cluster.


Nest Polymerase Chain Reaction Molecular Evolutionary Genetic Analysis Leaf Symptom Maximum Parsimony Method Australasian Plant Disease Note 
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  1. Deng S, Hiruki C (1991) Amplification of 16S rRNA genes from culturable and non-culturable mollicutes. Journal of Microbiological Methods 14, 53–61. doi:10.1016/0167-7012(91)90007-DCrossRefGoogle Scholar
  2. Gundersen DE, Lee I-M (1996) Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. Phytopathologia Mediterranea 35, 144–151.Google Scholar
  3. Khan A, Botti S, Al-Subhi A, Gundersen-Rindal D, Bertaccini A (2002) Molecular identification of a new phytoplasma associated with alfalfa witches’-broom in Oman. Phytopathology 92, 1038–1047. doi:10.1094/PHYTO.2002.92.10.1038PubMedCrossRefGoogle Scholar
  4. Martini M, Lee I-M, Bottner KD, Zhao Y, Botti S, Bertaccini A, Harrison NA, Carraro L, Marcone C, Khan AJ, Osler R (2007) Ribosomal protein gene-based phylogeny for finer differentiation and classification of phytoplasmas. International Journal of Systematic and Evolutionary Microbiology 57, 2037–2051. doi:10.1099/ijs.0.65013-0PubMedCrossRefGoogle Scholar
  5. Marzachi C, Coulibaly A, Coulibaly N, Sangare A, Diarra M, Gregorio TD, Bosco D (2009) Cotton virescence phytopasma and its weed reservoir in Mali. Journal of Plant Pathology 91, 717–721.Google Scholar
  6. Montano HG, Brioso PST, Cunha Jn JO, Figueiredo DV, Pimentel JP (2007) First report of group 16SrIII phytoplasma in loofah (Luffa cylindrica). Bulletin of Insectology 60, 277–278.Google Scholar
  7. Saghai-Maroof MA, Soliman KM, Jorgenson RA, Allard AW (1984) Ribosomal spacer-length polymorphism in barley: Mendelian inheritance, chromosomal creation and population dynamics. Proceedings of the National Academy of Sciences of the United States of America 81, 8014–8018. doi:10.1073/pnas.81.24.8014PubMedCrossRefGoogle Scholar
  8. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24, 1596–1599. doi:10.1093/molbev/msm092PubMedCrossRefGoogle Scholar

Copyright information

© Australasian Plant Pathology Society 2010

Authors and Affiliations

  1. 1.Department of BotanyUniversity of DelhiDelhiIndia

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