Australasian Plant Disease Notes

, Volume 5, Issue 1, pp 117–119 | Cite as

First report of ‘Candidatus Phytoplasma asteris’ (16SrI) associated with little leaf of cotton and luffa in India



Cotton and luffa plants with little leaf symptoms were observed during February 2010 at New Delhi, India. Nested polymerase chain reaction with phytoplasma 16S rDNA-specific primers efficiently detected the pathogen in the diseased plants. Sequence comparisons showed homology with the members of ‘Candidatus Phytoplasma asteris’, group 16SrI. Phylogenetic analysis also assigned the cotton and luffa little leaf phytoplasma to the 16SrI cluster.


Nest Polymerase Chain Reaction Molecular Evolutionary Genetic Analysis Leaf Symptom Maximum Parsimony Method Australasian Plant Disease Note 
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Copyright information

© Australasian Plant Pathology Society 2010

Authors and Affiliations

  1. 1.Department of BotanyUniversity of DelhiDelhiIndia

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