Skip to main content
SpringerLink
Log in

Molecular characterization of novel low-molecular-weight glutenin genes inAegilops longissima

  • Original Article
  • Published:
Journal of Applied Genetics Aims and scope Submit manuscript

Abstract

Extensive genetic variations of low-molecular-weight glutenin subunits (LMW-GS) and their coding genes were found in the wild diploid A- and D-genome donors of common wheat. In this study, we reported the isolation and characterization of 8 novel LMW-GS genes fromAe.longissima Schweinf. & Muschl., a species of the sectionSitopsis of the genusAegilops, which is closely related to the B genome of common wheat. Based on the N-terminal domain sequences, the 8 genes were divided into 3 groups. A consensus alignment of the extremely conserved domains with known gene groups and the subsequent cluster analysis showed that 2 out of the 3 groups of LMW-GS genes were closely related to those from the B genome, and the remaining was related to those from A and D genomes of wheat andAe. tauschii. Using 3 sets of gene-group-specific primers, PCRs in diploid, tetraploid and hexaploid wheats andAe. tauschii failed to obtain the expected products, indicating that the 3 groups of LMW-GS genes obtained in this study were new members of LMW-GS multi-gene families. These results suggested that theSitopsis species of the genusAegilops with novel gene variations could be used as valuable gene resources of LMW-GS. The 3 sets of group-specific primers could be utilized as molecular markers to investigate the introgression of novel alien LMW-GS genes fromAe. longissima into wheat.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bietz JA, Wall JS, 1973. Isolation and characterization of gliadin-like subunits from glutenins. Cereal Chem 50: 537–547.

    CAS  Google Scholar 

  • Blake N, Lehfeldt BR, Lavin M, Talbert LE, 1999. Phylogenetic reconstruction based on low copy DNA sequence data in an allopolyploid: The B genome of wheat. Genome 42: 351–360.

    Article  CAS  PubMed  Google Scholar 

  • Brites C, Carrillo JM, 2001. Influence of high molecular weight HMW and low molecular weight LMW glutenin subunits controlled byGlu-1 andGlu-3loci on durum wheat quality. Cereal Chem 78: 59–63.

    Article  CAS  Google Scholar 

  • Cassidy BG, Dvorak J, 1991. Molecular characterization of a low-molecular-weight glutenin cDNA clone fromTriticum durum. Theor Appl Genet 81: 653–660.

    Article  CAS  Google Scholar 

  • Cassidy BG, Dvorak J, Anderson OD, 1998. The wheat low-molecular-weight glutenin genes: characterization of 6 new genes and progress in understanding gene family structure. Theor Appl Genet 96: 743–750.

    Article  CAS  Google Scholar 

  • Ceoloni C, Del Signore G, Ercoli L, Donini P, 1992. Locating the alien chromatin segment in common wheat-A.longissima mildew-resistant transfer lines. Hereditas 116: 239–245.

    Google Scholar 

  • Ciaffi M, Lee YK, Tama SL, Gupta R, Skerritt J, Appels R, 1999. The low-molecular-weight glutenin subunit proteins of primitive wheats. III. The genes from D-genome species. Theor Appl Genet 98: 135–148.

    Article  CAS  Google Scholar 

  • D’Ovidio R, Marchitelli C, Ercoli Cardelli L, Porceddu E, 1999. Sequence similarity between allelicGlu-B3 genes related to quality properties of durum wheat. Theor Appl Genet 98: 455–461.

    Article  Google Scholar 

  • D’Ovidio R, Masci S, 2004. The low-molecular-weight glutenin subunits of wheat gluten. J Cereal Sci 39: 321–339.

    Article  Google Scholar 

  • Gianibelli MC, Wrigley CW, MacRitchie F, 2002. Polymorphism of low Mr Glutenin subunits inTriticum tauschii. Cereal Chem 35: 277–286.

    CAS  Google Scholar 

  • Gill BS, Sharma HC, Raupp WJ, Browder LE, Hatchett JH, Harvey TL, et al. 1985. Evaluation ofAegilops species for resistance to wheat powdery mildew, wheat leaf rust, Hessian fly and greenbug. Plant Dis 69: 314–316.

    Google Scholar 

  • Gupta RB, Paul JG, Cornish GB, Palmer G, Bekes F, Rathjen A, 1994. Allelic variation at glutenin subunit and gliadin loci,Glu-1, Glu-3 andGli-1, of common wheats. I. Its additive and interaction effects on dough properties. J Cereal Sci 19: 9–17.

    Article  CAS  Google Scholar 

  • Gupta RB, Singh NK, Shepherd KW, 1989.The cumulative effect of allelic variation in LMW and HMW glutenin subunits on physical dough properties in progeny of two bread wheats. Theor Appl Genet 77: 57–64.

    Article  CAS  Google Scholar 

  • Gupta RB, Bekes F, Wrigley CW, 1990a. Predicting values of LMW glutenin alleles for dough quality of bread wheat. In: Bushuk W, Tkachuk R, eds. Gluten proteins. American Association of Cereal Chemists, St. Paul, Minnesota, USA: 615–621.

    Google Scholar 

  • Gupta RB, Bekes F, Wrigley CW, Moss HJ, 1990b. Prediction of wheat dough quality in breeding onthe basis of LMW and HMW glutenin subunit composition. In: Proceedings of the 6th Australian Wheat Breeders Society Assembly, Tamworth, Australia: 217–225.

  • Gupta RB, Shepherd KW, 1988. LMW-GS in wheat: their variation, inheritance, and association with bread-making quality. In: Proceedings of the 7th International Wheat Genetics Symposium, Cambridge, UK: 943–949.

  • Huang SX, Sirikhachornkit A, Su XJ, Faris J, Gill B, Haselkorn R, and Gornicki P, 2002. Genes encoding plastid acetyl-CoA carboxylase and 3-phospho-glycerate kinase of theTriticum-Aegilops complex and the evolutionary history of polyploid wheat. Proc. Natl. Acad. Sci. USA 99: 8133–8138.

    Article  CAS  PubMed  Google Scholar 

  • Huang Z, Long H, Yan ZH, Wei YM, Zheng YL, 2005. Sequenceanalysis of LMW-GS genes fromAegilops tauschii containing HMW glutenin subunits 5+12 with good quality. High Technol Lett 15: 67–72.

    CAS  Google Scholar 

  • Ikeda TM, Nagamine T, Fukuoka H, Yano H, 2002. Identification of new low-molecular-weight glutenin subunit genes in wheat. Theor Appl Genet 104: 680–687.

    Article  CAS  PubMed  Google Scholar 

  • Jiang CX, Pei YH, Zhang YZ, Li XH, Yao DN, Yan YM, et al. 2008. Molecular cloning and characterization of four novel LMW glutenin subunit genes fromAegilops longissima, Triticum dicoccoides andT. zhukovskyi. Hereditas 145: 92–98.

    Article  PubMed  Google Scholar 

  • Johal J, Gianibelli MC, Rahman S, Morell MK, Gale KR, 2004. Characterization of low-molecular-weight glutenin genes inAegilops tauschii. Theor Appl Genet 109: 1028–1040.

    Article  CAS  PubMed  Google Scholar 

  • Kerby K, Kuspira J, 1988. Cytological evidence bearing on the origin of the B genome in polyploid wheats. Genome 30: 36–43.

    Google Scholar 

  • Kihara H, 1944. Discovery of the DD-analyser, one of the ancestors ofTriticum vulgare. Agric Hortic Tokyo. 19: 13–14.

    Google Scholar 

  • Kimber G, Feldman M, 1987. Wild wheat, an introduction. Columbia MO.: College of Agriculture. University of Missouri Special Report No. 353: 146.

  • Kwok S, Kellogg DE, McKinney N, Spasic D, Goda L, Levenson C, Sninsky JJ, 1990. Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 model studies. Nucleic Acids Res 18: 999–1005.

    Article  CAS  PubMed  Google Scholar 

  • Lee YK, Bekes F, Gupta R, Appels R, Morell MK, 1999a. The low-molecular-weight glutenin subunit proteins of primitive wheats. I. Variation in A-genome species. Theor Appl Genet 98: 119–125.

    Article  CAS  Google Scholar 

  • Lee YK, Ciaffi M, Appels R, Morell MK, 1999b. The low-molecular-weight glutenin subunit protein of primitive wheat. II. The genes from A-genome species. Theor Appl Genet 98: 126–134.

    Article  CAS  Google Scholar 

  • Long H, Wei YM, Yan ZH, Baum B, Nevo E, Zheng YL, 2005. Classification of wheat low-molecular-weight glutenin subunit genes and its chromosome assignment by developing LMW-GS group-specific primers. Theor Appl Genet 111: 1251–1259.

    Article  CAS  PubMed  Google Scholar 

  • Long H, Wei YM, Yan ZH, Baum B, Nevo E, Zheng YL, 2006. Analysis and validation of genome-specific DNA variations in 5’ flanking conserved sequences of wheat low-molecular-weight glutenin subunit genes. Sci in China Ser C-life Sci 49: 322–331.

    Article  CAS  Google Scholar 

  • Long H, Wei YM, Yan ZH, Zheng YL, 2004. Molecular cloning of a novel low-molecular-weight glutenin subunit gene from wheatTriticum aestivum L., variety ‘Chuannong 16’. Acta Agronomica Sinica 30: 1179–1184.

    CAS  Google Scholar 

  • Luo C, Griffin WB, Branlard G, McNeil DL, 2001. Comparison of low- and high-molecular-weight wheat glutenin allele effects on flour quality. Theor Appl Genet 102: 1088–1098.

    Article  CAS  Google Scholar 

  • Ma ZC, Wei YM, Long H, Yan ZH, Baum B, Zheng YL, 2006. Characterization of low-molecular-weight i-type glutenin subunit genes from diploid wheat in relation to the gene family structure. Molecular Biology Russian. 40: 897–906.

    CAS  Google Scholar 

  • Metakovsky EV, Wrigley CW, Bekes F Gupta RB, 1990. Gluten polypeptides as useful genetic markers of dough qualityin Australian wheats. Aust J Agric Res 41: 289–306.

    Article  Google Scholar 

  • Morris R, Sears ER, 1967. The cytogenetics of wheat and its relatives. In: Quisenberry KS, Reitz LP, eds. Wheat and wheat improvement. Madison Wisconsin. American Society of Agronomy: 19–87.

    Google Scholar 

  • Murray M, Thompson WF, 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8: 4321–4325.

    Article  CAS  PubMed  Google Scholar 

  • Pogna N E, Autran JC, Mellini F, Lafiandra D, Feillet P, 1990. Chromosome 1B-encoded gliadins and glutenin subunits in durum wheat: genetics and relationship to gluten strength. J Cereal Sci 11: 15–34.

    Article  CAS  Google Scholar 

  • Ruiz M, Carrillo JM, 1993. Linkage relationships between prolamins genes on chromosomes 1A and 1B of durum wheat. Theor Appl Genet 87: 353–360.

    Article  CAS  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T, 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York.

    Google Scholar 

  • Sarkar P, Stebbins GL, 1956. Morphological evidence concerning the origin of the Bgenome in wheat. Am J Bot 43: 297–304.

    Article  Google Scholar 

  • Shang HY, Wei YM, Long H, Yan ZH, Zheng YL, 2005. Identification of LMW glutenin-like genes fromSecale sylvestre. Host. Russ J Genet 41: 1–9.

    Google Scholar 

  • Shewry PR, Tatham AS, Barro F, Barcelo P, Lazzeri P, 1995. Biotechnology of bread-making: unraveling and manipulating the multi-protein gluten complex. Biotechnology 13: 1185–1190.

    Article  CAS  Google Scholar 

  • Shewry PR, Halford NG, Tatham AS, 1992. The high molecular weight subunits of wheat glutenin. J Cereal Sci 15: 105–120.

    Article  CAS  Google Scholar 

  • Singh NK, Shepherd KW, 1988. The structure and genetic control ofa new classof disulphide-linked proteins in wheat endosperm. Theor Appl Genet 71: 79–92.

    Article  Google Scholar 

  • Wang, JR, Yan ZH, Jiang QT, Wei YM, Baum BR, Zheng YL, 2007. Sequence variations and molecular phylogenetic analyses of the HMW-GS genes from different genomes in Triticeae. Biochem Syst Ecol 35: 421–433.

    Article  CAS  Google Scholar 

  • Wang ZQ, Long H, Zheng YL, Yan ZH, Wei YM, Lan XJ, 2005. Cloning and analysis of LMW-GS genes fromTriticum aestevum ssp.Tibetanum Shao. Acta Genetica Sinica 32: 86–93.

    CAS  PubMed  Google Scholar 

  • Wesley AS, Lukow OM, McKenzie RIH, Ames N, Brown D, 2001. Effect of multiple substitution of glutenin or gliadin proteins on flour quality of Canada Prairie Spring wheat. Cereal Chem 78: 69–73.

    Article  CAS  Google Scholar 

  • Yan ZH, Wan YF, Liu KF, Zheng YL, Wang DW, 2002. Identification of a novel HMW glutenin subunit and comparison of its amino acid sequence with those of homologous subunits. Chin Sci Bull 47: 220–225.

    Article  Google Scholar 

  • Yue YW, Long H, Liu Q, Wei YM, Yan ZH, Zheng YL, 2005. Isolation of low-molecular-weight glutenin subunit genes from wild emmer wheatTriticum dicoccoides. J Appl Genet 46: 349–355.

    PubMed  Google Scholar 

  • Zhang W, Gianibelli MC, Rampling LR, Gale KR, 2004. Characterisation and marker development for low molecular weight gluten in genes fromGlu-A3 alleles of bread wheatTriticum aestivum. L. Theor Appl Genet 108: 1409–1419.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dujiangyan Campus, Sichuan Agricultural University, Dujiangyan, Sichuan, China

    Z. Huang

  2. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, China

    H. Long

  3. Triticeae Research Institute, Sichuan Agricultural University, Yaan, Sichuan, China

    Z. Huang, Q. T. Jiang, Y. M. Wei, Z. H. Yan & Y-L. Zheng

  4. Ministry of Education Key Laboratory for Crop Genetic Resources and Improvement in South-west China, Sichuan Agricultural University, 625014, Yaan, Sichuan, China

    Y-L. Zheng

Authors
  1. Z. Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Long
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Q. T. Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Y. M. Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Z. H. Yan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Y-L. Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Y-L. Zheng.

Additional information

The first two authors contributed equally to this paper.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Huang, Z., Long, H., Jiang, Q.T. et al. Molecular characterization of novel low-molecular-weight glutenin genes inAegilops longissima . J Appl Genet 51, 9–18 (2010). https://doi.org/10.1007/BF03195705

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03195705

Keywords

Search

Navigation