Journal of Applied Genetics

, Volume 51, Issue 1, pp 9–18 | Cite as

Molecular characterization of novel low-molecular-weight glutenin genes inAegilops longissima

  • Z. Huang
  • H. Long
  • Q. T. Jiang
  • Y. M. Wei
  • Z. H. Yan
  • Y-L. Zheng
Original Article


Extensive genetic variations of low-molecular-weight glutenin subunits (LMW-GS) and their coding genes were found in the wild diploid A- and D-genome donors of common wheat. In this study, we reported the isolation and characterization of 8 novel LMW-GS genes fromAe.longissima Schweinf. & Muschl., a species of the sectionSitopsis of the genusAegilops, which is closely related to the B genome of common wheat. Based on the N-terminal domain sequences, the 8 genes were divided into 3 groups. A consensus alignment of the extremely conserved domains with known gene groups and the subsequent cluster analysis showed that 2 out of the 3 groups of LMW-GS genes were closely related to those from the B genome, and the remaining was related to those from A and D genomes of wheat andAe. tauschii. Using 3 sets of gene-group-specific primers, PCRs in diploid, tetraploid and hexaploid wheats andAe. tauschii failed to obtain the expected products, indicating that the 3 groups of LMW-GS genes obtained in this study were new members of LMW-GS multi-gene families. These results suggested that theSitopsis species of the genusAegilops with novel gene variations could be used as valuable gene resources of LMW-GS. The 3 sets of group-specific primers could be utilized as molecular markers to investigate the introgression of novel alien LMW-GS genes fromAe. longissima into wheat.


Aegilops longissima DNA marker LMW-GS gene S1 genome Sitopsis 


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Copyright information

© Institute of Plant Genetics, Polish Academy of Sciences, Poznan 2010

Authors and Affiliations

  • Z. Huang
    • 1
    • 3
  • H. Long
    • 2
  • Q. T. Jiang
    • 3
  • Y. M. Wei
    • 3
  • Z. H. Yan
    • 3
  • Y-L. Zheng
    • 3
    • 4
  1. 1.Dujiangyan CampusSichuan Agricultural UniversityDujiangyan, SichuanChina
  2. 2.Chengdu Institute of BiologyChinese Academy of SciencesChengdu, SichuanChina
  3. 3.Triticeae Research InstituteSichuan Agricultural UniversityYaan, SichuanChina
  4. 4.Ministry of Education Key Laboratory for Crop Genetic Resources and Improvement in South-west ChinaSichuan Agricultural UniversityYaan, SichuanChina

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