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Journal of Applied Genetics

, Volume 49, Issue 3, pp 237–250 | Cite as

Gene expression profiling in skeletal muscle of Holstein-Friesian bulls with single-nucleotide polymorphism in the myostatin gene 5’-flanking region

  • Tomasz Sadkowski
  • Michał Jank
  • Lech Zwierzchowski
  • Eulalia Siadkowska
  • Jolanta Oprządek
  • Tomasz Motyl
Original Article

Abstract

Myostatin (GDF-8) is a key protein responsible for skeletal muscle growth and development, thus mutations in themstn gene can have major economic and breeding consequences. The aim of the present study was to investigate myostatin gene expression and transcriptional profile in skeletal muscle of Holstein-Friesian (Black-and-White) bulls carrying a polymorphism in the 5’-flanking region of themstn gene (G/C transversion at position -7828). Real-time qRT-PCR and cDNA microarray revealed significantly lowermstn expression in muscle of bulls with the CC genotype, as compared to GG and GC genotypes. The direct comparison of skeletal muscle transcriptional profiles between the CC genotype and GG and GC genotypes resulted in identification of genes, of which at least some can be putative targets for myostatin. Using cDNA microarray, we identified 43 common genes (includingmstn) with significantly different expression in skeletal muscle of bulls with the CC genotype, as compared to GG and GC genotypes, 15 of which were upregulated and 28 were downregulated in the CC genotype. Classification of molecular function of differentially expressed genes revealed the highest number of genes involved in the expression of cytoskeleton proteins (9), extracellular matrix proteins (4), nucleic acid-binding proteins (4), calcium-binding proteins (4), and transcription factors (4). The biological functions of the largest number of genes involved: protein metabolism and modification (10), signal transduction (10), cell structure (8), and developmental processes (8). The main identified signaling pathways were: Wnt (4), chemokines and cytokines (4), integrin (4), nicotine receptor for acetylocholine (3), TGF-beta (2), and cytoskeleton regulation by Rho GTPase (2). We identified previously unrecognized putatively myostatin-dependent genes, encoding transcription factors (EGR1, Nf1b, ILF1), components of the proteasomal complex (PSMB7, PSMD13) and proteins with some other molecular function in skeletal muscle (ITGB1BP3, Pla2g1b, ISYNA1, TNFAIP6, MST1, TNNT1, CALB3, CACYBP, and CTNNA1).

Keywords

cattle DNA microarray myostatin polymorphism skeletal muscle transcriptome 

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Copyright information

© Institute of Plant Genetics, Polish Academy of Sciences, Poznan 2008

Authors and Affiliations

  • Tomasz Sadkowski
    • 1
  • Michał Jank
    • 1
  • Lech Zwierzchowski
    • 2
  • Eulalia Siadkowska
    • 2
  • Jolanta Oprządek
    • 2
  • Tomasz Motyl
    • 1
  1. 1.Department of Physiological Sciences, Faculty of Veterinary MedicineWarsaw University of Life Sciences (SGGW)WarsawPoland
  2. 2.Institute of Genetics and Animal BreedingPolish Academy of SciencesJastrzębiecPoland

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