A novel mitochondrial intergenic spacer reflecting population structure of Pacific oyster
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Nucleotide sequence divergence in a novel major mitochondrial DNA intergenic spacer (IGS) of Pacific oysterCrassostrea gigas was analyzed for 29 cultured individuals within the Goseong population (Korea). A total of 7 variable sites were detected within the IGS, and the relative frequency of nucleotide alteration was determined to be 1.16%. All alterations were due to a single nucleotide substitution, and 5 transitions and 2 transversions were observed. Among 29 specimens, only 8 haplotypes could be identified, and 6 of the haplotypes were unique to particular specimens. Pairwise genetic diversity of all 8 haplotypes was calculated to be 0.412 ± 0.134 from multiple sequence substitutions based on the two-parameter model. The phylogenetic tree obtained for these haplotypes according to the neighbor-joining method illustrated a single cluster of linkages, which comprised 5 haplotypes associated with 23 specimens, while the other 3 haplotypes associated with 6 specimens were scattered. The results indicate that the IGS is higher polymorphic and thus more suitable as a genetic marker for population structure analysis of Pacific oyster than the mtDNA coding regions, such as cytochromec oxidase I and 16S ribosomal RNA genes.
Key wordsCrassostrea gigas intergenic spacer mitochondrial DNA noncoding region Pacific oyster
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- Chew KK, 1990. Global bivalve shellfish introductions. World Aquaculture 21: 9–22.Google Scholar
- FAO, 1997. Review of the state of world aquaculture. FAO Fisheries Circular 886, FAO, Rome.Google Scholar
- Imai T, Sakai S, 1961. Study of breeding of Japanese oyster,Crassostrea gigas. Tohoku J Agric Res 12: 125–171.Google Scholar
- Okimoto T, 2006. Comparative genetics for putative control regions of mitochondrial DNA in Pacific oyster. Ph.D. Thesis, Nagasaki University, Nagasaki, Japan.Google Scholar
- Park BH, Park MS, Kim BY, Hur SB, Kim SJ, 1989. Culture of the Pacific Oyster (Crassostrea gigas) in the Republic of Korea. FAO, Rome.Google Scholar
- Rana K, Immink A, 2001. Trends in global aquaculture production: 1984–1996. FAO, Rome.Google Scholar