Summary
The toxicity of twenty-fourFusarium isolates was evaluated towards larvae ofGalleria mellonella by the antlfeedant activity of the autoclaved fungal culture incorporated into insect diet. Culturesof isolates belonging to F.sporotrichioldes, F. compactum andF. sambucinum, well known as capable to synthesize trichothecenes, showed high antifeedant activity (ca 60–70%); whereas controversial activity was showed byF. equiseti, F. proliferatum andF. solani cultures, and not significant activity was showed byF. oxysporum cultures. The G.mellonella larvae bioassay is suggested as a reliable sensitive Insectbioassay for screening fungal toxicity.
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References
Campadelli G (1973) Allevamento diGalleria mellonella L. (Lepidoptera: Galleriidae) con dieta semiartificiale. Boll Ist Univ Bologna 32: 11–25
Claydon N, Grove F (1984)Fusarium as an insect pathogen. In. The Applied Mycology ofFusarium Moss MO and Smith JE (eds). Cambridge University Press, Cambridge, 117–128.
Davis GRF, Smith JD, Schiffer B, Loew FM (1975) Screening for mycotoxins with larvae ofTenebrio molitor. J Invertebr Pathol 26: 299–303
Dowd PF (1989) Toxicity of naturally occurring levels of thePenicillium mycotoxins citrinin, ochratoxin A, and penicillic acid to the com earworm,Heliothis zea, and the fall armyworm,Spodoptera frugiperda (Lepidoptera: Noctuidae). Environ Entomol 18(1): 24–29
Dowd PF (1992) Insect interactions with mycotoxin-producing fungi and their hosts.In: Handbook of Applied Mycology. Bhatnagar D, Lillehoj EB and Arora DK (eds) Marcel Dekker, New York, Basel, Hong Kong, 5:137–155
Grove JF, Hosken M (1975) The larvicidal activity of some 12,13-epoxy-trichothec-9-enes. Biochem Pharmacol 24:959–962
Grove, JF, Pople M (1980) The insecticidal activity of beauvericin and the enniatin complex. Mycopathologia 70, 103–105
Logrieco A, Chelkowski J, Bottalico A, Visconti A (1990) Further data on specific trichothecene production byFusarium sect.Sporotrichiella strains. Mycol Res 94:587–589
Marasas, WFO, Nelson PE, Toussoun TA(1984). ToxigenicFusarium species — Identity and mycotoxicology. The Pennsylvania State University Press, University Park, 328
Matsumura F, Knight G (1967) Toxicity and chemosterilizing activity of aflatoxin against insects. J Econ Entomol, 60(3): 871–873
McMillian WW, Wilson DM, Widstrom NW, Perkins WD (1980) Effects of aflatoxin B1 and G1 on three insect pest of maize. J Econ Entomol 73:26–28
Mulè G, D’Ambrosio A, Logrieco A, Bottalico A (1992) Toxicity of mycotoxins ofFusarium sambucinum for feeding inGalleria mellonella. Entomol Exp Appl 62: 17–22
Nelson PE, Toussoun TA, Marasas WFO (1983)Fusarium species — An illustrated manual for identification. Pennsylvania State University Press, University Park, PA, USA, 193 p
Paterson RRM, Simmonds MJS, Kemmelmeier C, Blaney WM (1990) Effect of brevianamide A, its photolysis product brevianamide D, and ochratoxin A from twoPenicillium strains on the insect pestSpodoptera frugiperda. Mycol Res 94: 538–542
Reiss J (1973) Toxicity of molds to the larvae ofTenebrio molitor. J Invertebr Pathol 21: 112–113
Scott PM (1984) Effects of food processing on mycotoxins. J Food Protection 47: 489–499
Sinha RN (1966) Development and mortality ofTribolium castaneum andT. confusum (Coleoptera: Tenebrioides) on seed-borne fungi. Annals Entomological Society of America 59: 192–201
Strongman DB, Strunz Gm, Yu C-M, Calhoun L (1988) Enniatins fromFusarium avenaceum isolated from balsam fir foliage and their toxicity to spruce budworm larvae,Choristoneura fumiferana (Clem.) (Lepidoptera: Tortricidae). J Chem Ecol 14: 753–764
Toscano NC, Reeves EL (1973) Effect ofAspergillus flavus mycotoxins on Culex mosquito larvae. J Invertebr Pathol 22: 55–59
Ueno Y (1983) Trichothecenes- chemical, biological and toxicological aspects. Tokyo: Kodansha Ltd & Amsterdam: Elsevier Sei Pub BV, 313 p
Visconti A, Mirocha CJ, Bottalico A, Chelkowski J (1985) Trichothecene mycotoxins produced byFusarium sporotrichioides strain P-ll. Mycotoxin Res 1:3–10
Visconti A, Mirocha CJ, Logrieco A, Bottalico A, Solfrizzo M (1989) Mycotoxins produced byFusarium acuminatum. Isolation and characterization of acuminatin: a new trichothecene. J Agric Food Chem 37:1348–1351
Wicklow DT, Dowd PF, Tepaske MR, Gloer JB, (1988) Sclerotial metabolites ofAspergillus flavus toxic to detritivourus maize insect (Carpophilus hemipterus, Nitidulidae). Trans Br mycol Soc 91(3): 433–438
Wright VF, De las Casas E, Harein PK (1976) The response ofTribolium confusum to the mycotoxins zearalenone (F-2) and T-2 toxin. Environ Entomol 5: 371–374
Wright VF, Karein PK, Collins NA (1980) Preference of the confused flour beetle for certainPenicillium isolates. Environ Entomol 9: 213–216
Wright VF, Vesonder RF, Ciegler A (1982) Mycotoxins and other fungal metabolites as insecticides.In: On Microbial and Viral Pesticides, Kurstak E (ed), Dekker, New York & Basel: 559–583
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Mulè, G., Logrieco, A., Bottalico, A. et al. Screening forFusarium toxigenic isolates using larvae ofGalleria mellonella . Mycotox Res 10, 41–46 (1994). https://doi.org/10.1007/BF03192249
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DOI: https://doi.org/10.1007/BF03192249