Mycotoxin Research

, Volume 9, Issue 1, pp 22–26 | Cite as

Inhibition of aflatoxin-induced liver damage in ducklings by food additives

  • Soni K B 
  • Rajan A 
  • Kuttan R 


Inhibitory effects of food additives on toxicity induced by aflatoxin B1 was conducted in 3-day-old ducklings. Aflatoxin B1 at a dose of 5 μg/day per animal for 14 days induced severe liver damage which included necrosis, fatty changes, and biliary hyperplasia. These changes were found to be inhibited by the daily administration of turmeric (50mg), curcumin (10 mg), and ellagic acid (10 mg) in the diet. Addition of BHA-butylated hydroxy anisole (10 mg), BHT-butylated hydroxy toluene (10 mg), garlic (500 mg), and asafoetida (50 mg) inhibited necrosis and degeneration of the tissue, while biliary hyperplasia persisted. Biochemical and haematological parameters were not significantly altered under the conditions studied.


Curcumin Aflatoxin Food Additive Ellagic Acid Butylate Hydroxy Toluene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Robinson P (1967) Infantile cirrhosis of the liver in India with special reference to probable aflatoxin etiology. Clin Pediatr 6: 57–62CrossRefGoogle Scholar
  2. 2.
    Becroft DMO, Webster DR (1972) Aflatoxin and Rye’s syndrome. Br Med J 114–117Google Scholar
  3. 3.
    Essigman JM, Croy RG, Bennet RA, and Wogan GN (1982) Metabolic activation of Aflatoxin B1: Pattern of DNA adduct formation, removal and excretion in relation to carcinogenesis. Drug Metabol Rev 13: 581–602CrossRefGoogle Scholar
  4. 4.
    Swenson DH, Miller EC, and Miller JA (1974) Aflatoxin B1 2, 3 — epoxide: evidence for its formation in rat liver in vivo and by human liver microsomes in vitro. Biochem Biophys Res Commun 60: 1036–1043CrossRefPubMedGoogle Scholar
  5. 5.
    Mandai S, Ahuya A, Shivapurkar NM, Cheng S-J, Groopman JD, and Stoner GD (1987) Inhibition of aflatoxin B1 mutagenesis in Salmonella typhimurium and DNA damage in cultured rat and human tracheobronchial tissues by ellagic acid. Carcinogenesis 8: 1651–1656CrossRefGoogle Scholar
  6. 6.
    Francis AR, Shetty TK, and Bhattacharya RK (1989) Modifying role of dietary factors on the mutagenicity of Aflatoxin B1; in vitro effect of plant flavanoids. Mutat Res 222: 393–401CrossRefPubMedGoogle Scholar
  7. 7.
    Unnikrishnan MC and Kuttan R (1990) Tumour reducing and anti-carcinogenic activity of selected spices. Cancer Letters 51: 85–89CrossRefPubMedGoogle Scholar
  8. 8.
    Determination of haemoglobin by cyanmethaemoglobin method (1980)In: Practical Clinical Biochemistry, 5th edn; Varley H, Gowenlock AH, and Bell M (eds): William Heinemann Medical Books, Ltd, London 1, 979Google Scholar
  9. 9.
    Bergmeyer HU and Bernt E (1980) Colorimetric method for aspartate and alanine amino transferases.In: Practical Clinical Biochemistry, 5th edn; Varley H, Gowenlock AH, and Bell M (eds). William Heinemann Medical Books, Ltd, London 1, 741–742Google Scholar
  10. 10.
    King EJ and Amstrong AR (1980) Method of King and Armstrong.In: Practical Clinical Biochemistry, 5th edn, Varley H, Gowenlock AH, and Bell M (eds). William Heinemann Medical Books, Ltd, London 1, 897–899Google Scholar
  11. 11.
    Bishayee A, Balasubramanian AS (1971) Lipid peroxide in rat brain. J Neurochem 18:909–920CrossRefPubMedGoogle Scholar
  12. 12.
    Bhattacharya RK, Firozi PF, and Aboobacker VS (1984) Factors modulating the formation of DNA adduct by Aflatoxin B1 in vitro. Carcinogen 5: 1359–1362CrossRefGoogle Scholar
  13. 13.
    Soudamini KK and Kuttan R (1988) Cytotoxic and tumour reducing properties of curcumin. Indian J Pharmacol 20: 95–101Google Scholar
  14. 14.
    Firozi PF, Aboobaker VS, and Bhattacharya RK (1986) Modulation by dertain factors of metabolic activation of aflatoxin B1 as detected in vitro in a simple fluorimetric assay. Chem Biol Interact 58: 173–184CrossRefPubMedGoogle Scholar
  15. 15.
    Williams GM, Tanaka T, and Maccura Y (1986) Dose — related inhibition of aflatoxin B1 induced hepatocarcinogenesis by the phenolic antioxidants, butylated hydroxy anisole, and butylated hydroxy toluene. Carcinogen 7: 1043–1050CrossRefGoogle Scholar

Copyright information

© Society of Mycotoxin Research and Springer 1993

Authors and Affiliations

  • Soni K B 
    • 1
  • Rajan A 
    • 2
  • Kuttan R 
    • 1
  1. 1.Amala Cancer Research CentreIndia
  2. 2.Dept of PathologyVeterinary CollegeIndia

Personalised recommendations