Advertisement

Mycotoxin Research

, Volume 6, Issue 2, pp 85–88 | Cite as

Fumonisin B1: Isolation from corn culture, and purification by high performance liquid chromatography

  • R Vesonder
  • R Peterson
  • R Plattner
  • D Weisleder
Article

Abstract

A method is described to isolate fumonisin B1 (FB1) from corn cultured for 18 days at 25°C withFusarium moniliforme. Cultured corn was extracted with aqueous methanol and purified with XAD-2 column chromatography and high performance liquid chromatography (HPLC). About 450 mg of FB1 were obtained from 800g cultured corn. Its identity was established by fast-atom bombardment (FAB) mass spectrometry, and infrared spectrum and nuclear magnetic spectrum. Its purity was estimated to be 95% by gas chromatography/mass spectrometry (GC/MS).References

Keywords

High Performance Liquid Chromatography Mycotoxin Research Fusarium Moniliforme Purple Spot Corn Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Bezuidenhout SC, Gelderblom WCA, Gorst-Allman CP, Horak RM, MarasasWFO, Spiteller G, and Vleggaar R (1988) Structure elucidation of the fumonisins, mycotoxins fromFusarium moniliforme. J Chem Soc, Chem Commun 743–745Google Scholar
  2. 2.
    Gelderblom WCA, Jaskiewicz K, Marasas WFO, Thiel PG, Horak RM, Vleggaar R and Kriek NPJ (1988) Fumonisins — novel mycotoxins with cancer-promoting activity produced byFusarium moniliforme. Appl Environ Microbiol 54:1806–1811PubMedGoogle Scholar
  3. 3.
    Marasas WFO, Kellerman TS, Gelderblom WCA, Coetyer JAW, Thiel PG, and Van der Lugt JJ (1988) Leukoencephalomalacia in a horse induced by fumonisin B1 isolated fromFusarium moniliforme. Onderstepoort J Vet Res 55:197–203PubMedGoogle Scholar
  4. 4.
    Plattner RD, Norred WP, Bacon CW, Voss KA, Peterson RE, Shackelford DD, and Weisleder D (1990) A method of detection of fumonisins in corn samples associated with field cases of equine leukoencephalomalacia. Mycologia accepted for November/December issue.Google Scholar

Copyright information

© Society of Mycotoxin Research and Springer 1990

Authors and Affiliations

  • R Vesonder
    • 1
  • R Peterson
    • 1
  • R Plattner
    • 1
  • D Weisleder
    • 1
  1. 1.Mycotoxin Research, US Department of Agriculture, Agricultural Research ServiceNorthern Regional Research CenterPeoriaUSA

Personalised recommendations