Production of14C-ochratoxin A byPenicillium verrucosum sp. 1761 in liquid culture
- 2 Downloads
The synthesis of14C-labelled ochratoxin A was carried out by biosynthesis withPénicillium verrucosum sp. 1761 in liquid culture using 2-14C-Na-acetate and 2-14C-malonic acid as precursor. The largest amount of ochratoxin A produced byPénicillium verrucosum was detected in a minimal medium after an incubation period of 28 days. By addition of citric acid and 2,4-dichlorophenoxy acetic acid larger ochratoxin A contents could be measured. For transformation studies of ochratoxin A, a molecule which is exclusively labelled in the isocoumarin moiety is best suitable. This labelling could be obtained by using labelled malonic acid as precursor, whereas with 2-14C-Na-acetate as precursor the whole molecule was labelled. Incorporation rates of radioactivity into the molecule were from 0.04% to 0.11%, specific radioactivities from 0.02 μCi/mg to 0.07 μCi/mg.
KeywordsCitric Acid Aflatoxin Malonic Acid Incorporation Rate Mycotoxin Research
Unable to display preview. Download preview PDF.
- 1.Aydes J, Mateles RI, 1964.Incorporation of labelled compounds into aflatoxins. Biochim Biophys Acta 86, 418–420Google Scholar
- 4.Cohen H, Lapointe M, 1986.Determination of ochratoxin A in animal feed and cereal grains by liquid chromatography with fluorescence detection. J Ass Off Anal Chem 69, 957–959Google Scholar
- 7.Königer M, Süß A, 1968. The use of gelating agents in assaying substances labelled with C14. Kerntechnik, Isotopentechnik und Chemie 5, 274–278Google Scholar
- 8.Lillehoj EB, Aalund O, Haid B, 1978. Bioproduction of14C-ochratoxin A in submerged culture. Appl Environ Microbiol 3, 720–723Google Scholar
- 11.Roberts JC, Woolven P, 1970. Studies in mycological chemistry. Part XXIV. Synthesis of ochratoxin A, a metabolite ofAspergillus ochraceus Wilh. J Chem Soc C, 278–281Google Scholar
- 15.Van der Merwe KJ, Steyn PS, Fourie L, 1965. Mycotoxins. Part II. The constitution of ochratoxin A, B, and C, metabolites ofAspergillus ochraceus Wilh. J Chem Soc 7083–7088Google Scholar