Mycotoxin Research

, Volume 12, Issue 1, pp 7–13 | Cite as

Production of14C-ochratoxin A byPenicillium verrucosum sp. 1761 in liquid culture

  • M Ruhland
  • G Engelhardt
  • P R Wallnöter


The synthesis of14C-labelled ochratoxin A was carried out by biosynthesis withPénicillium verrucosum sp. 1761 in liquid culture using 2-14C-Na-acetate and 2-14C-malonic acid as precursor. The largest amount of ochratoxin A produced byPénicillium verrucosum was detected in a minimal medium after an incubation period of 28 days. By addition of citric acid and 2,4-dichlorophenoxy acetic acid larger ochratoxin A contents could be measured. For transformation studies of ochratoxin A, a molecule which is exclusively labelled in the isocoumarin moiety is best suitable. This labelling could be obtained by using labelled malonic acid as precursor, whereas with 2-14C-Na-acetate as precursor the whole molecule was labelled. Incorporation rates of radioactivity into the molecule were from 0.04% to 0.11%, specific radioactivities from 0.02 μCi/mg to 0.07 μCi/mg.


Citric Acid Aflatoxin Malonic Acid Incorporation Rate Mycotoxin Research 
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Copyright information

© Society of Mycotoxin Research and Springer 1996

Authors and Affiliations

  • M Ruhland
    • 1
  • G Engelhardt
    • 1
  • P R Wallnöter
    • 1
  1. 1.Bayerische Landesanstalt für ErnährungMünchenGermany

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