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Mycotoxin Research

, Volume 12, Issue 1, pp 7–13 | Cite as

Production of14C-ochratoxin A byPenicillium verrucosum sp. 1761 in liquid culture

  • M Ruhland
  • G Engelhardt
  • P R Wallnöter
Article

Summary

The synthesis of14C-labelled ochratoxin A was carried out by biosynthesis withPénicillium verrucosum sp. 1761 in liquid culture using 2-14C-Na-acetate and 2-14C-malonic acid as precursor. The largest amount of ochratoxin A produced byPénicillium verrucosum was detected in a minimal medium after an incubation period of 28 days. By addition of citric acid and 2,4-dichlorophenoxy acetic acid larger ochratoxin A contents could be measured. For transformation studies of ochratoxin A, a molecule which is exclusively labelled in the isocoumarin moiety is best suitable. This labelling could be obtained by using labelled malonic acid as precursor, whereas with 2-14C-Na-acetate as precursor the whole molecule was labelled. Incorporation rates of radioactivity into the molecule were from 0.04% to 0.11%, specific radioactivities from 0.02 μCi/mg to 0.07 μCi/mg.

Keywords

Citric Acid Aflatoxin Malonic Acid Incorporation Rate Mycotoxin Research 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Society of Mycotoxin Research and Springer 1996

Authors and Affiliations

  • M Ruhland
    • 1
  • G Engelhardt
    • 1
  • P R Wallnöter
    • 1
  1. 1.Bayerische Landesanstalt für ErnährungMünchenGermany

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