Mycotoxin Research

, Volume 3, Issue 2, pp 69–76 | Cite as

Toxicity of epoxy trichothecenes in cultured mammalian cells

  • KE von Milczewski


With four established cell lines, cytotoxicity of 23 epoxy trichothecenes was tested. Three cell lines from human origin have been found to have a similar degree of sensitivity to trichothecenes. A pig kidney cell line had a higher sensitivity to NT-1 toxin, neosolaniol, tetraacetyl T-2, and iso-T-2 toxin. The potential cause for discrepancies in literature were discussed. Activity of esterases in calf serum used for cell cultures should be recorded whenever conducting toxicity tests in cell cultures with trichothecenes.

Relations of chemical structures of toxins to their biological activities were investigated. With verrucarol (scirpendiol), scirpentriol, and T-2 tetraol analogues the esters generally showed significantly higher cytotoxicity. But, loss of acetyl groups at single positions did not necessarily mean decline in toxicity. With macrocyclic trichothecenes minor differences in the side chain of verrucarol esters were found to contribute to differences in biological activity.


Trichothecene Patulin Cholesterol Oxidase Mycotoxin Research Chang Liver Cell 
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  1. 1.
    Saito M, K Ohtsubo, M Umeda, M Enemoto, H Kurata, S Udagawa, F Sakabe, M Ichinoe (1971) Screening tests using HeLa cells and mice for detection of mycotoxin-producing fungi isolatedfrom foodstuffs. Japan J Exp Med 41:1–20Google Scholar
  2. 2.
    Kawasaki I, T Oko, M Umeda, M Saito (1972) Cytotoxic effect of penicillic acid and patulin in HeLa cells. Japan J Exp Med 42:327–340Google Scholar
  3. 3.
    von Milczewski KE, U Krusch (1976) deUntersuchungen überdie Beeinflussung von Zellkulturen menschlicher Herkunft durch Mykotoxine: cytomorphologische Veränderungen in Abhängigkeit von der Toxinkonzentration. Kieler Milchw Forsch Ber 28:391–457Google Scholar
  4. 4.
    Waters R (1984) DNA repair test in cultured mammalian cells.In: “Mutagenicity testing”. S Venitt & JM Parry eds. IRL Press, OxfordGoogle Scholar
  5. 5.
    Williams GM (1976) Carcinogen-induced DNA repair in primary rat liver cultures; a possible screen for chemical carcinogens. Cancer Lett 1:231–236CrossRefPubMedGoogle Scholar
  6. 6.
    von Milczewski KE (1982) Fluctuation of the sensitivity of mammalian cell lines in bioassay procedures for mycotoxins known to be activated by metabolic conversion. Proceedings of the V. Intern IUPAC Symposium on Mycotoxins and Phycotoxins, 257-260Google Scholar
  7. 7.
    Lompe A, von Milczewski KE (1979) Ein Zellkulturtest für den Nachweis von Mykotoxinen. Z Lebensm Unters Forsch 169:249–254CrossRefPubMedGoogle Scholar
  8. 8.
    Ueno Y (1983) Biological detection of trichothecenes. In: “Trichothecenes”. Y Ueno (ed) Elsevier, AmsterdamGoogle Scholar
  9. 9.
    Robb J, M Norval (1983) Comparison of cytotoxicity and thin-layer chromatography methods for detection of mycotoxins. Appl Environ Microbiol 46:948–950PubMedGoogle Scholar
  10. 10.
    Bergemeyer HU (1970) Methoden der enzymatischen Analyse, Vol 1, 2. Auflage, Verlag Chemie, Weinheim (a) W Pilz: Arylesterasen, 773–780. (b) K Walter, Ch Schütt: Saure und alkalische Phosphatasen im Serum. 818–822Google Scholar
  11. 11.
    Johann H (1986) Dissertation, MainzGoogle Scholar
  12. 12.
    Mirocha ChJ (1986) Metabolism and residue of trichothecene toxins in animals and plant systems. In: “Mycotoxins and phycotoxins”. PS Steyn & R Vleggar (eds) Elsevier Science Publishers, AmsterdamGoogle Scholar
  13. 13.
    Haley S, RP Geyer (1961) Comparison of lipid metabolism of chicken embryo organs and cell cultures. Proc Soc Exp Biol Med 108:6–9Google Scholar

Copyright information

© Society of Mycotoxin Research and Springer 1987

Authors and Affiliations

  • KE von Milczewski
    • 1
  1. 1.Institut für MikrobiologieBundesanstalt für MilchforschungKielFRG

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