Mycotoxin Research

, Volume 3, Issue 1, pp 25–32 | Cite as

On the metabolism of the mycotoxin deoxynivalenol in the isolated perfused rat liver

  • M Gareis
  • J Bauer
  • B Gedek


An isolated rat liver was perfused with deoxynivalenol (DON) at a dose of 3 mg in a recirculating perfusion system. To identify glucuronide conjugates equal amounts of bile samples, perfusate and liver homogenates were incubated with and without (control) a β-glucuronidase preparation and analyzed by thin layer chromatography and capillary gas liquid chromatography — chemical ionization mass spectrometry. A total of 40.4% of the administered dose of DON was found to be conjugated with glucuronic acid (perfusate 20.4%, bile 19.2%, liver 0.8%), while only 1.3% of the parent DON (perfusate 1.1%, bile 0.2%) was detected. The cleavage of DON-glucuronide was demonstrated by incubating DON-glucuronide containing bile samples with intestine contents under anaerobic conditions.


Trichothecene Deoxynivalenol Mycotoxin Research Bile Sample Trichothecene Mycotoxin 
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Copyright information

© Society of Mycotoxin Research and Springer 1987

Authors and Affiliations

  • M Gareis
    • 1
  • J Bauer
    • 1
  • B Gedek
    • 1
  1. 1.Institute for Medical Microbiology, Infectious and Epidemic Diseases Faculty of Veterinary MedicineUniversity of MunichMunich 22FRG

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