Patulin degradation in saccharomyces cerevisiae: Sensitive mutants
The present experiments (sensitive mutants and transient inhibition of growth) are compatible with the synthesis of an inductible detoxifying substance in the wild type strain. This substance could be glutathione because glutathione detoxification scheme essentially involves properties of the SH group and it is well known that patulin reacts with sulfhy dril groups.
Studies are presently being carried out with sensitive mutants to establish definitively the relation between intracellular pool of glutathone and the resistance mechanism of a yeast to patulin.
KeywordsSaccharomyces Cerevisiae Wild Type Strain Alloxan Patulin Mycotoxin Research
Unable to display preview. Download preview PDF.
- 1.Sumbu ZL, Thonart Ph, and Bechet J (1982) Action of patulin on yeast. Appl Environ Microbiol 45:110–115.Google Scholar
- 3.Sumbu ZL, Thonart Ph, Wathelet JP, Blanpain P, and Bechet J (1981) La Patuline-Production, purification, degradation et dosage par CPG. Rev Ferment Ind Mm 36:122–129.Google Scholar
- 4.Paterson JW and Lazarow A (1955) Determination of glutathione, in: Methods of Biochemical Analysis. Dabidglich Vol II, 259.Google Scholar
- 5.Scott WT and Bullerman LB (1975) Patulin: a mycotoxin of potential concern in foods. J Milk Food Techn 38:695–705.Google Scholar
- 6.Thonart Ph and Bechet J (1980) Recherche de mutants sensibles à la patuline chez la levure. Communication 52.4.2 (P) 5th International Symposium on Yeast. London, Canada, 225 – 230.Google Scholar
- 7.Halden JT (1962) Amino acid pools distribution, formation, and function of free amino acids. Elsevier Publishing Company, 82-83.Google Scholar