Production of 3-acetyldeoxynivalenol in shake culture
In order to produce deoxynivalenol (DON), conditions for the production of 3-acetyldeoxynivalenol (Ac-DON) in shake culture were studied. A selected isolate of DON-producer,F. graminearum strain R2118, produced about 70mg/mL of Ac-DON when cultured at 25°C for 4 days in a medium consisting of 3% sucrose, 0.1% peptone, and 0.1% yeast extract. From the mass culture (10 L) of the strain, 792 mg of Ac-DON were isolated and 384 mg of DON were obtained after hydrolysis of the acetate.
KeywordsGraminearum Sucrose Concentration Trichothecene Fusarium Species Zearalenone
Unable to display preview. Download preview PDF.
- 1.Morooka N, Uratsuji N, Yoshizawa T, Yamamoto H (1972) Studies on the toxic substances in barley infected withFusarium species. J Food Hyg Soc Japan 13:368–375.Google Scholar
- 2.Vesonder RF, Ciegler A, Jensen AH (1973) Isolation of the emetic principle fromFusarium-infected corn. Appl Env Microbiol 26:1008–1010.Google Scholar
- 3.Vesonder RF, Ciegler A, Jensen AH, Rohwedder WK, Weisleder D (1976) Coidentity of the refusal and emetic principle fromFusarium-infected corn. Appl Env Microbiol 31:280–285.Google Scholar
- 4.Mirocha CJ, Schauerhamer B, Pathre SV, Christensen CM (1976) Natural occurrence ofFusarium toxins in feedstuff. Appl Env Microbiol 32:535–556.Google Scholar
- 5.Vesonder RF, Ciegler A, Rogers RF, Burbridge KA, Bothast RJ, Jensen AH (1978) Survey of 1977 crop year preharvest corn for vomitoxin. Appl Env Microbiol 36:885–888.Google Scholar
- 11.Yoshizawa T, Hosokawa H (1983) Natural occurrence of deoxynivalenol and nivalenol, trichothecene mycotoxins, in commercial foods. J Food Hyg Soc Japan 24:413–415.Google Scholar
- 12.Hagler WM, Tyczkowska K, Hamilton PB (1984) Simultaneous occurrence of deoxynivalenol, zearalenone, and aflatoxin in 1982 scabby wheat from the midwestern United States. Appl Env Microbiol 47:151–154.Google Scholar
- 13.Côté LM, Reynold JD, Vesonder RF, Buck WB, Swanson SP, Coffey RT, Brown DC (1984) Survey of vomitoxin-contaminated feed grains in midwestern United States, and associated health problems in swine. J Amer Vet Med Assoc 184: 189–192.Google Scholar
- 15.Ichinoe M, Kurata H, Sugiura Y, Ueno Y (1983) Chemo taxonomy ofGibberella zeae with special reference to production of trichothecenes and zearalenone. Appl Env Microbiol 46:1364–1369.Google Scholar