Mycotoxin Research

, Volume 1, Issue 1, pp 19–24 | Cite as

Production of 3-acetyldeoxynivalenol in shake culture

  • K. Ishii
  • H. Sato
  • Y. Ueno


In order to produce deoxynivalenol (DON), conditions for the production of 3-acetyldeoxynivalenol (Ac-DON) in shake culture were studied. A selected isolate of DON-producer,F. graminearum strain R2118, produced about 70mg/mL of Ac-DON when cultured at 25°C for 4 days in a medium consisting of 3% sucrose, 0.1% peptone, and 0.1% yeast extract. From the mass culture (10 L) of the strain, 792 mg of Ac-DON were isolated and 384 mg of DON were obtained after hydrolysis of the acetate.


Graminearum Sucrose Concentration Trichothecene Fusarium Species Zearalenone 
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Copyright information

© Society of Mycotoxin Research and Springer 1985

Authors and Affiliations

  • K. Ishii
    • 1
  • H. Sato
    • 1
  • Y. Ueno
    • 1
  1. 1.Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical SciencesScience University of TokyoTokyoJapan

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