Genes & Genomics

, Volume 31, Issue 5, pp 349–359 | Cite as

The mitochondrial DNA control region comparison studies of four hinged turtles and its phylogentic significance of the genusCuora sensu lato (Testudinata: Geoemydidae)

  • Yanyun Zhang
  • Liuwang Nie
  • Yuqing Huang
  • Youguang Pu
  • Li Zhang


The complete sequences of the mitochondrial DNA (mtDNA) control region (CR) ofCistoclemmys flavomarginata, Cistoclemmys galbinifrons, Cuora aurocapitata andCyclemys atripons were amplified by long-polymerase chain reaction (Long-PCR). The lengths were 1207 bp, 1722 bp, 1379 bp and 980 bp, respectively. Combining with the CR sequence ofPyxidea mouhotii (DQ659152), we compared the CR structure, and identified three functional domains (TAS, CD and CSB) in which the conservation sequences (TAS, CSB-F, CSB-1, CSB-2 and CSB-3) were also successfully identified according to their homology to those of other turtles. These 5 turtles have the identical CSB-2 and CSB-3 sequences, and 4 of them have the same CSB-1 sequence while there is one base transversion (T → A) inCy. atripons. We analyzed the variable number of tandem repeat (VNTR) sequences or microsatellites at the 3′ end of CR. The motifs of tandem repeats (7 types) are made up of 2–8 nucleotides, and the copy numbers are from 4 to 48. All of the 5 turtles exceptCy. atripons have the “TATTATAT” repeats and are ended by TA. The results of CR structure analysis displayed that theCuora, Cistoclemmys, andPyxidea have many similarities, but differ fromCyclemys. WithIndotestudo elongate (DQ080043) andIndotestudo forstenii (DQ080044) as outgroups, using the CR sequences (1123bp) excluded the microsatellites at the 3′ end of CR, we constructed the molecular phylogenetic trees using the MP, ML and BI methods. The results showed that there was a strong support to the monophyly of theCuora group consisting ofCuora,Cistoclemmys andPyxidea, which has a close relationship withMauremys andChinemys but far fromCyclemys, which are consistent with the analysis of the CR structure of the 5 turtles.

Key words

Geoemydidae Cuora group mitochondrial DNA control region microsatellites phylogeny 


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Copyright information

© The Genetics Society of Korea & Springer 2009

Authors and Affiliations

  • Yanyun Zhang
    • 1
  • Liuwang Nie
    • 1
  • Yuqing Huang
    • 1
  • Youguang Pu
    • 1
  • Li Zhang
    • 1
  1. 1.Life Science CollegeAnhui Normal University, The Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in AnhuiWuhu, AnhuiChina

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