Metabolism of the new nonbenzodiazepine anxiolytic agent, RWJ-51204, in mouse, rat, dog, monkey and human hepatic S9 fractions, and in rats, dogs and humans
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The in vitro and in vivo metabolism of the nonbenzodiazepine anxiolytic agent, RWJ-51204 was investigated after incubation with mice, rat, dog, monkey, and human hepatic S9 fractions in the presence of NADPH-generating system, and a single oral dose administration to rats (100 mg/kg), dogs (5 mg/kg), and humans (2.5 mg/subject). Plasma and red blood cells (2 h, rat) and urine samples (0–24 h, rat, dog and human) were obtained postdose. Unchanged RWJ-51204 (39–93% of the sample in vitro; ≤5% of the sample in vivo) plus 14 metabolites were profiled, quantified and tentatively identified on the basis of API-MS and MS/MS data, and by comparison of synthetic samples.
The in vitro and in vivo metabolic pathways for RWJ-51204 are proposed, and the metabolite formations are via the following five pathways: 1. phenyl oxidation, 2. pyrido-oxidation, 3. N-deethoxymethylation, 4. dehydration, and 5. glucuronidation. Pathway 1 formed 4-hydroxy-2-fluoro-phenyl-RWJ-51204 (Ml, 7–24% in vitro; 5–60% in vivo) in major amounts, OH-benzimidazole-RWJ-51204 (M2, 5–8% in vitro and in vivo) and diOH-phenyl-RWJ-51204 (≤5–16% in vitro and in vivo); in conjunction with pathway 5 produced Ml glucuronide (60% in rat & dog; 17% in human), M2 glucuronide (16% in human). Pathways 2–4 formed minor/trace oxidized, and dehydrated metabolites. RWJ-51204 is extensively metabolized in vitro (except dog) and in vivo in rats, dogs and humans.
KeywordsRWJ-51204 anxiolytic agent in vitro & in vivo metabolism animals human ms & ms/ms metabolite identification
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