Does stress influence ampicillin concentration in serum and tissues?
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Exercise produces changes of drug levels in plasma and increases the concentration of free fatty acids (FFAs), which may interfere with drug-protein binding. FFAs seem to play an antagonistic role to drugs since they have a strong binding capacity to serum albumin. The aim of this study was to evaluate the influence of the consecutive exercise-induced stress in ampicillin levels. Two groups of Wistar rats were used. Group A consisted of six subgroups that were subjected to cold swimming (4°C) for 5, 10, 15, 20, 25, 30 days respectively. Group B was the control group. The animals were injected im. with ampicillin (1 g/Kg/8h in 5 doses). Results showed that exercise enhanced stress parameters (FFAs, adrenal weight, Ht%) and led to an ampicillin increase in all experimental groups comparatively to controls.
KeywordsCold swimming stress ampicillin concentration serum and tissue
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- 1.Hurwitz G, Webb J, Walle T, Bai S, Daniell H, Courley L. (1983): Exercise induced increments in plasma levels of proprandol and noradrenaline. Br J Pharmacol; 16: 599–608Google Scholar
- 5.Giagnoni G, Santagostino A, Senini R, Fumagalli P, Cori E. (1983): Cold 013 stress in the rat induces changes in plasma levels of endorphin and ACTH. Pharm Research Communications 15(1) 0139 0208Google Scholar
- 6.Santagastino A, Giagnoni G, Panlraia A, Denti M, Paresi D, Fumagalli P, Gorris I. (1982): Changes in endorphin content induced by cold stress in the rat pituitary gland. IRCS Sci 10: 173–179Google Scholar
- 11.Starjec JJ, Barger DF, Hesse R. (1983): Effect of stress and exercise on plasma corticosterone, plasma cholesterol and aortic cholesterol levels in rats. Psychosomatic Medicine 45(3): 219–226Google Scholar
- 13.Schach D. (1969): The influence of physical stress and exercise on growth or more and insuline secretion. J Clin Med 69: 256–259Google Scholar
- 14.Suldow G, Birkett D, Wade D. (1976): Further characterization of specific drug binding sites on human serum albumin. Mol Pharmacol 12: 1052–1062Google Scholar
- 15.Achbrook J.D, Spector A.A., Santos E.C, Fletcher J.E. (1975): Long chain fatty acid binding to human plasma albumin. J Biol Chem 250: 2333–2338Google Scholar
- 16.Bennet V.J, Brodie L.J, Benner L.E, Kirby NW. (1966): Simplified accurte method to antibiotic assay of clinical specimens. App. Microbiol 14: 170–177Google Scholar
- 20.Groenewald JV, Terblanche SE, Oelofsen W. (1980): Effect of prolonged exercise on certain anatomical and biochemical parameters in male and female rats. African Sports Research. 1: 43–52Google Scholar
- 21.Jurgens JL, Kirillov OI. (1972): Mitotic activity of the cells of the adrenal cortex of rats subjected to prolonged hypokinesia. Bulletin of Exper Biol Med 74: 98Google Scholar
- 24.Goldstein DS, Dionne R, Sweet J, Gracely R, Brewer HB Jr, Gregg R, Kaiser HR. (1982): Circulatory plasma catecholamine, cortisol lipid and psychological responses to a real life stress (wisdom tooth extractios). Effects of diazepam sedation and inclusion of epinephrine with the local anesthetic. Psychosomatic Medicine 44(3): 259–271PubMedGoogle Scholar
- 28.Dettli L. Spring P. (1966): Diurnal variations in the elimination rate of sulfonamide in man. Helv Med Acta 33: 291–306Google Scholar
- 29.Schmidt H, Roholt K. (1959): Penicillin serum concentrations in relation to exercise. Acta Path Microbiol Scand 68: 396–399Google Scholar
- 32.Neusholme E.A., Leech A.R. 1983: Biochemistry on the Medical Science. Edition. Willy and sons, pp 571,Google Scholar
- 33.Sellers, E.M. 1978: The clinical importance of interactions based on displacement of protein bound drugs. Pro. Int. Cong. Pharm 2, pp 896Google Scholar