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Does stress influence ampicillin concentration in serum and tissues?

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Summary

Exercise produces changes of drug levels in plasma and increases the concentration of free fatty acids (FFAs), which may interfere with drug-protein binding. FFAs seem to play an antagonistic role to drugs since they have a strong binding capacity to serum albumin. The aim of this study was to evaluate the influence of the consecutive exercise-induced stress in ampicillin levels. Two groups of Wistar rats were used. Group A consisted of six subgroups that were subjected to cold swimming (4°C) for 5, 10, 15, 20, 25, 30 days respectively. Group B was the control group. The animals were injected im. with ampicillin (1 g/Kg/8h in 5 doses). Results showed that exercise enhanced stress parameters (FFAs, adrenal weight, Ht%) and led to an ampicillin increase in all experimental groups comparatively to controls.

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References

  1. Hurwitz G, Webb J, Walle T, Bai S, Daniell H, Courley L. (1983): Exercise induced increments in plasma levels of proprandol and noradrenaline. Br J Pharmacol; 16: 599–608

    CAS  Google Scholar 

  2. Ylitalo P, Hinkka H, Neuvonen PJ. (1977): Effect of exercise on the serum level and urinary excretion of tetracycline, doxycycline and sulphamethizole. Eur J Clin Pharmacol 12: 367–373

    Article  CAS  PubMed  Google Scholar 

  3. Powis G, Snow DH. (1978): The effects of exercise and adrenaline infusion upon the blood levels of propranolon and antipyrine in the horse. J Pharmacol Exp Ther 205(3): 725–731

    CAS  PubMed  Google Scholar 

  4. Papadopoulos NM, Bloor CM, Standefer JC. (1969): Effects of exercise and training on plasma lipids and lipoproteins in the rats. J Appl Physiol 26: 760–763

    CAS  PubMed  Google Scholar 

  5. Giagnoni G, Santagostino A, Senini R, Fumagalli P, Cori E. (1983): Cold 013 stress in the rat induces changes in plasma levels of endorphin and ACTH. Pharm Research Communications 15(1) 0139 0208

  6. Santagastino A, Giagnoni G, Panlraia A, Denti M, Paresi D, Fumagalli P, Gorris I. (1982): Changes in endorphin content induced by cold stress in the rat pituitary gland. IRCS Sci 10: 173–179

    Google Scholar 

  7. Tsopanakis C, Kostarellis D, Dontas I, Karayannacos P, Skalkeas G. (1988): Effects of cold stress on serum lipids lipoproteins and the activity of LCAT in rabbits. Bioch Med Metabol Biol 39: 148–157

    Article  CAS  Google Scholar 

  8. Connell A.M., Cooper J., Redfearn J.W. (1958): The contrasting effects of emotional tension and physical exercise on the excretion of 17-ketogenic steroids and 17-ketosteroids. Acta Endocrinol 27: 179–194

    CAS  PubMed  Google Scholar 

  9. Rosch JP. (1983): Stress, cholesterol and coronary heart disease. The Lancet Oct: 851–852

    Article  Google Scholar 

  10. Rivier C, Vale W. (1983): Modulation of stress induced ACTH release of corticotropin-releasing factor catecholamines and vasopresin. Nature 305: 325–327

    Article  CAS  PubMed  Google Scholar 

  11. Starjec JJ, Barger DF, Hesse R. (1983): Effect of stress and exercise on plasma corticosterone, plasma cholesterol and aortic cholesterol levels in rats. Psychosomatic Medicine 45(3): 219–226

    Google Scholar 

  12. Crane A. (1969): Anterior pituitary junction during surgical stress. J Clin Endocrinol 29: 63–67

    Article  Google Scholar 

  13. Schach D. (1969): The influence of physical stress and exercise on growth or more and insuline secretion. J Clin Med 69: 256–259

    Google Scholar 

  14. Suldow G, Birkett D, Wade D. (1976): Further characterization of specific drug binding sites on human serum albumin. Mol Pharmacol 12: 1052–1062

    Google Scholar 

  15. Achbrook J.D, Spector A.A., Santos E.C, Fletcher J.E. (1975): Long chain fatty acid binding to human plasma albumin. J Biol Chem 250: 2333–2338

    Google Scholar 

  16. Bennet V.J, Brodie L.J, Benner L.E, Kirby NW. (1966): Simplified accurte method to antibiotic assay of clinical specimens. App. Microbiol 14: 170–177

    Google Scholar 

  17. Kurz H. (1961): Ein Geraet zur Herstellun duenner Schnitte aus frischem tiereschem Gewebe. Z Ges: Exp Med 134, 304–308

    Article  CAS  Google Scholar 

  18. Munck A, Guyre PM, Holbrook NI. (1984): Physiological functions of glucocorticoids in stress and their relationship to pharmacological actions. Endocrine Reviews 5: 25–44

    Article  CAS  PubMed  Google Scholar 

  19. Selye H, (1946): J Clin Endocrin Metabol 6: 117

    Article  CAS  Google Scholar 

  20. Groenewald JV, Terblanche SE, Oelofsen W. (1980): Effect of prolonged exercise on certain anatomical and biochemical parameters in male and female rats. African Sports Research. 1: 43–52

    Google Scholar 

  21. Jurgens JL, Kirillov OI. (1972): Mitotic activity of the cells of the adrenal cortex of rats subjected to prolonged hypokinesia. Bulletin of Exper Biol Med 74: 98

    Google Scholar 

  22. Jurgens JL, Kirillov OI. (1970): Mitotic activity of the cells of the adrenal cortex of rats subjected to prolonged exercise. Bulletin of Exper Biol Med 69: 100

    Article  Google Scholar 

  23. Sfikakis A., Galanopoulou P, Konstandi M., Tsakayannis D. (1996): Stress through handling for vaginal screening, serotonin and Acth response to ether. Pharm Biochem Behav 53(4): 965–970

    Article  CAS  Google Scholar 

  24. Goldstein DS, Dionne R, Sweet J, Gracely R, Brewer HB Jr, Gregg R, Kaiser HR. (1982): Circulatory plasma catecholamine, cortisol lipid and psychological responses to a real life stress (wisdom tooth extractios). Effects of diazepam sedation and inclusion of epinephrine with the local anesthetic. Psychosomatic Medicine 44(3): 259–271

    CAS  PubMed  Google Scholar 

  25. Tsopanakis C, Tesseromatis C. (1991): Cold swimming stress: Effects on serum lipids, lipoproteins and LCAT activity in male and female rats. Pharmacol Bioch Behav 38: 813–816

    Article  CAS  Google Scholar 

  26. Mason JW. (1968): A review of phycoendocrine research on the pituitary adrenal cortisol system. Psychosomatic Medicine, 30, 576

    PubMed  Google Scholar 

  27. Konig E, Lemp A. (1966): Plasmavolumenaenderungen durch alltaglich Belastungen Herzgesunden und Herzinsuffizienten. Klin Wochenschr 44: 862–870

    Article  CAS  PubMed  Google Scholar 

  28. Dettli L. Spring P. (1966): Diurnal variations in the elimination rate of sulfonamide in man. Helv Med Acta 33: 291–306

    Google Scholar 

  29. Schmidt H, Roholt K. (1959): Penicillin serum concentrations in relation to exercise. Acta Path Microbiol Scand 68: 396–399

    Google Scholar 

  30. Hermansen L, Osnes JB. (1972): Blood and muscle pH after maximal exercise in man. J Appl Physiol 32: 304–308

    CAS  PubMed  Google Scholar 

  31. Bennet W., Walker R., Henry J., Kincaid F. (1983): Chronic interstial nephropathy is mice induced by psycosocial stress. Nephron 34: 110–113

    Article  Google Scholar 

  32. Neusholme E.A., Leech A.R. 1983: Biochemistry on the Medical Science. Edition. Willy and sons, pp 571,

  33. Sellers, E.M. 1978: The clinical importance of interactions based on displacement of protein bound drugs. Pro. Int. Cong. Pharm 2, pp 896

    Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacology, Medical School, University of Athens, Coudi 11527, Athens, Greece

    Tesseromatis C., Trichilis A., Tsivos E., Messari J., Triantaphyllidis H. & Varonos D. D.

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  1. Tesseromatis C.
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  2. Trichilis A.
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  3. Tsivos E.
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  4. Messari J.
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  5. Triantaphyllidis H.
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  6. Varonos D. D.
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Tesseromatis, C., Trichilis, A., Tsivos, E. et al. Does stress influence ampicillin concentration in serum and tissues?. Eur. J. Drug Metab. Pharmacokinet. 26, 167–171 (2001). https://doi.org/10.1007/BF03190392

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  • DOI: https://doi.org/10.1007/BF03190392

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