Does stress influence ampicillin concentration in serum and tissues?
Exercise produces changes of drug levels in plasma and increases the concentration of free fatty acids (FFAs), which may interfere with drug-protein binding. FFAs seem to play an antagonistic role to drugs since they have a strong binding capacity to serum albumin. The aim of this study was to evaluate the influence of the consecutive exercise-induced stress in ampicillin levels. Two groups of Wistar rats were used. Group A consisted of six subgroups that were subjected to cold swimming (4°C) for 5, 10, 15, 20, 25, 30 days respectively. Group B was the control group. The animals were injected im. with ampicillin (1 g/Kg/8h in 5 doses). Results showed that exercise enhanced stress parameters (FFAs, adrenal weight, Ht%) and led to an ampicillin increase in all experimental groups comparatively to controls.
KeywordsCold swimming stress ampicillin concentration serum and tissue
Unable to display preview. Download preview PDF.
- 1.Hurwitz G, Webb J, Walle T, Bai S, Daniell H, Courley L. (1983): Exercise induced increments in plasma levels of proprandol and noradrenaline. Br J Pharmacol; 16: 599–608Google Scholar
- 5.Giagnoni G, Santagostino A, Senini R, Fumagalli P, Cori E. (1983): Cold 013 stress in the rat induces changes in plasma levels of endorphin and ACTH. Pharm Research Communications 15(1) 0139 0208Google Scholar
- 6.Santagastino A, Giagnoni G, Panlraia A, Denti M, Paresi D, Fumagalli P, Gorris I. (1982): Changes in endorphin content induced by cold stress in the rat pituitary gland. IRCS Sci 10: 173–179Google Scholar
- 11.Starjec JJ, Barger DF, Hesse R. (1983): Effect of stress and exercise on plasma corticosterone, plasma cholesterol and aortic cholesterol levels in rats. Psychosomatic Medicine 45(3): 219–226Google Scholar
- 13.Schach D. (1969): The influence of physical stress and exercise on growth or more and insuline secretion. J Clin Med 69: 256–259Google Scholar
- 14.Suldow G, Birkett D, Wade D. (1976): Further characterization of specific drug binding sites on human serum albumin. Mol Pharmacol 12: 1052–1062Google Scholar
- 15.Achbrook J.D, Spector A.A., Santos E.C, Fletcher J.E. (1975): Long chain fatty acid binding to human plasma albumin. J Biol Chem 250: 2333–2338Google Scholar
- 16.Bennet V.J, Brodie L.J, Benner L.E, Kirby NW. (1966): Simplified accurte method to antibiotic assay of clinical specimens. App. Microbiol 14: 170–177Google Scholar
- 20.Groenewald JV, Terblanche SE, Oelofsen W. (1980): Effect of prolonged exercise on certain anatomical and biochemical parameters in male and female rats. African Sports Research. 1: 43–52Google Scholar
- 21.Jurgens JL, Kirillov OI. (1972): Mitotic activity of the cells of the adrenal cortex of rats subjected to prolonged hypokinesia. Bulletin of Exper Biol Med 74: 98Google Scholar
- 24.Goldstein DS, Dionne R, Sweet J, Gracely R, Brewer HB Jr, Gregg R, Kaiser HR. (1982): Circulatory plasma catecholamine, cortisol lipid and psychological responses to a real life stress (wisdom tooth extractios). Effects of diazepam sedation and inclusion of epinephrine with the local anesthetic. Psychosomatic Medicine 44(3): 259–271PubMedGoogle Scholar
- 28.Dettli L. Spring P. (1966): Diurnal variations in the elimination rate of sulfonamide in man. Helv Med Acta 33: 291–306Google Scholar
- 29.Schmidt H, Roholt K. (1959): Penicillin serum concentrations in relation to exercise. Acta Path Microbiol Scand 68: 396–399Google Scholar
- 32.Neusholme E.A., Leech A.R. 1983: Biochemistry on the Medical Science. Edition. Willy and sons, pp 571,Google Scholar
- 33.Sellers, E.M. 1978: The clinical importance of interactions based on displacement of protein bound drugs. Pro. Int. Cong. Pharm 2, pp 896Google Scholar