Excretion of Aflatoxin B1 as a glutathione conjugate
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Intraperitoneal injection of3H-GSH (15.4 mg, 0,5 μCi) 15 minutes before a similar i.p. treatment with Aflatoxin B1 (AFB1) (2mg/kg) showed less than 40% of the radioactivity in the 24 h urine and 30–35% in the bile. Analysis of the urine and bile samples by TLC and separation with XAD-2, showed only traces of the AFB1-GSH adduct present in the urine and 14% in the bile. AFB1 metabolites isolated from the urine of the3H-GSH treated rats were identical with those of rats treated with AFB1 only. While GSH Conjugate appears therefore to be a major component of biliary excretion of AFB1, it does not seem to be significant in the urinary excretion of the toxin.
Key wordsAflatoxin B1 glutathione conjugation biliary and urinary excretion
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