Excretion of Aflatoxin B1 as a glutathione conjugate

  • Godwin O. Emerole


Intraperitoneal injection of3H-GSH (15.4 mg, 0,5 μCi) 15 minutes before a similar i.p. treatment with Aflatoxin B1 (AFB1) (2mg/kg) showed less than 40% of the radioactivity in the 24 h urine and 30–35% in the bile. Analysis of the urine and bile samples by TLC and separation with XAD-2, showed only traces of the AFB1-GSH adduct present in the urine and 14% in the bile. AFB1 metabolites isolated from the urine of the3H-GSH treated rats were identical with those of rats treated with AFB1 only. While GSH Conjugate appears therefore to be a major component of biliary excretion of AFB1, it does not seem to be significant in the urinary excretion of the toxin.

Key words

Aflatoxin B1 glutathione conjugation biliary and urinary excretion 


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  1. 1.
    Newberne, P.M. and Butler, W.H. (1969) Cancer Res. 29, 236–250. Acute and chronic effects of aflatoxin on the liver of Domestic and laboratory animals. A. review.PubMedGoogle Scholar
  2. 2.
    Garner, R.C. Miller, E.C. and Miller, J.A. (1972) Liver microsomal metabolism of aflatoxin B1 to a reactive derivative toxic to Salmonella Typhimurium TA 1530 Cancer Res. 32, 2058–2066.PubMedGoogle Scholar
  3. 3.
    Garner, R.C. (1973) Chemical evidence for the forma-tion of a reactive aflatoxin B1 metabolite, by hamster liver microsomes. Febs. Lett. 36: 261–264.CrossRefPubMedGoogle Scholar
  4. 4.
    Garner R.C. and Wright CM. (1975) Binding of (14C) aflatoxin B1 to cellular macromolecules in the rat and hamster Chem — Biol. Interactions. II, 123–131.CrossRefGoogle Scholar
  5. 5.
    Gurtoo, H.L. and Dave, C. (1975). Invitro metabolic Conversion of aflatoxins and Benzo(a)pyrene to Nucleic acid binding metabolites. Cancer Res. 35, 382–389.PubMedGoogle Scholar
  6. 6.
    Miller, E.C. and Miller, J.A. (1966) Mechanisms of chemical Carcinogenesis. Nature of proximate carcinogens and interactions with macromolecules. Pharmacological Reviews 18, 805–837.PubMedGoogle Scholar
  7. 7.
    Miller, J.A. (1970). Carcinogenesis by chemicals: An overview- G.H.A. Clowes Memorial Lecture. Cancer Res. 30, 559–576.PubMedGoogle Scholar
  8. 8.
    Miller, J.A. and Miller E.C. (1977). The concept of reactive electrophilic metabolites in chemical carcino-genesis: Recent results with aromatic amines, safrole and as aflatoxin B1 In Biological Reactive intermediates: formation, toxicity and interactions Edited by Jollow, D.J. Kocsis, J.J. Synder, R. and Vainio, T.J., 6–24 Plenum press, N. York, London.Google Scholar
  9. 9.
    Mitchell, J.R., Jollow, D.J. Potter, W.Z., Gillette, J.R. and Brodie, B.B. (1973) Acetaminophen-induced hepatic necrosis iv. Protective role of glutathione. J. Pharmacol. Exptl. Ther. 187, 211–217.Google Scholar
  10. 10.
    Jollow, D.J., Mitchell, J.R., Zampaglione, N. and Gillette, J.R., (1974) Bromobenzene-Liver necrosis: Protective role of glutathione and evidence for 2.4 Bromobenzene oxide as the hepatotoxic metabolite Pharmacology 11:157–169.CrossRefGoogle Scholar
  11. 11.
    Chasseud, L.F. (1976) Glutathione: Metabolism and function. Edited by Aras. I.M. and Jakoby, W.B. Raven Press, New York, pp 77–114.Google Scholar
  12. 12.
    White, I.N.H. (1976) The role of liver glutathione in the acute Toxicity of retrosine to rats. Chem. Biol. Inter. 13, 333–342.CrossRefGoogle Scholar
  13. 13.
    Emerole G.O., Neskovic, N. and Dixon, R.L. (1979) The detoxication of aflatoxin B1 with glutathione. Xenobiotica 9, 737–743.CrossRefPubMedGoogle Scholar
  14. 14.
    Ryan, A.J. and Bend, J.R. (1977) The metabolism of styrene oxide in the isolated perfused rat liver. Identification and quantitation of major metabolites. Amer. soc. pharmacol Exptl. Ther., 5(4) 363–367.Google Scholar
  15. 15.
    Bassir, O. and Osiyemi, F. (1967) Biliary excretion of aflatoxin in the rat after a single dose. Nature 215, 882.CrossRefPubMedGoogle Scholar
  16. 16.
    Wogan, G.N., Edwards, G.S. Shank, R.C. (1967) Excretion and Tissue Distribution of Radioactivity from aflatoxin B1-14C in rats cancer Res. 27: 1729–1736.Google Scholar
  17. 17.
    Smith, R.L. (1973) The excretary function of bile: The elimination of drugs and toxic substances in bile. Chapman and hall London.Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Godwin O. Emerole
    • 1
  1. 1.Biochemistry DepartmentUniversity of IbadanIbadanNigeria

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