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Excretion of Aflatoxin B1 as a glutathione conjugate

  • Godwin O. Emerole
Article

Summary

Intraperitoneal injection of3H-GSH (15.4 mg, 0,5 μCi) 15 minutes before a similar i.p. treatment with Aflatoxin B1 (AFB1) (2mg/kg) showed less than 40% of the radioactivity in the 24 h urine and 30–35% in the bile. Analysis of the urine and bile samples by TLC and separation with XAD-2, showed only traces of the AFB1-GSH adduct present in the urine and 14% in the bile. AFB1 metabolites isolated from the urine of the3H-GSH treated rats were identical with those of rats treated with AFB1 only. While GSH Conjugate appears therefore to be a major component of biliary excretion of AFB1, it does not seem to be significant in the urinary excretion of the toxin.

Key words

Aflatoxin B1 glutathione conjugation biliary and urinary excretion 

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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Godwin O. Emerole
    • 1
  1. 1.Biochemistry DepartmentUniversity of IbadanIbadanNigeria

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