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Capillary electrophoresis and Capillary Electrophoresis-mass spectrometry in drug and metabolite analysis

  • Stephen Naylor
  • Andy J. Tomlinson
  • Linda M. Benson
  • John W. Gorrod
Article

Summary

The structural diversity of modern therapeutic agents can lead to labour intensive method development for each drug and the structural characterization of their metabolites. In this work, we show the benefits of the high resolution capabilities of capillary electrophoresis (CE) and demonstrate that nonaqueous CE and on-line CE-mass spectrometry (CE-MS) leads to enhanced resolution and recovery of mixtures containing the prototype H2-antagonist, mifentidine, and putative metabolites. Furthermore, the usefulness of CE-tandem MS (CE-MS/MS) is also demonstrated by the structural characterization of the novel N2-hydroxylamine metabolite of mifentidine.

Keywords

capillary electrophoresis capillary electrophoresis-mass spectrometry tandem MS drug metabolism 

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Stephen Naylor
    • 1
    • 2
  • Andy J. Tomlinson
    • 1
  • Linda M. Benson
    • 1
  • John W. Gorrod
    • 3
  1. 1.Biomedical Mass Spectrometry Facility, Department of Biochemistry and Molecular BiologyGuggenheim Mayo ClinicRochesterUSA
  2. 2.Department of PharmacologyMayo ClinicUSA
  3. 3.Chelsea Department of Pharmacy, King's College LondonUniversity of LondonUK

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