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Annals of Vascular Surgery

, Volume 3, Issue 1, pp 8–10 | Cite as

Plasma level of the creatine phosphokinase BB isoenzyme during experimental intestinal ischemia

  • Vassilios E. Smirniotis
  • Alexandra T. Labrou
  • Dimitris D. Tsiftses
Original Articles
  • 6 Downloads

Abstract

Changes in the serum of CPK-BB isoenzyme were investigated in dogs with intesti-nal ischemia in order to determine their use as an enzymatic diagnostic test for bowel Infarction. The study consisted of 12 mongrel dogs. Group I (n = 6) was assigned as a control and was subjected to lumen obstruction of a jejunal segment 50–60 cm in length. Group II (n = 6) had an intestinal infarction created by ligation of the arteries of a jejunal segment 50–60 cm in length. A catheter was placed into the right atrium through the left jugular vein for blood sampling and monitoring. Blood samples were withdrawn every three hours for biochemical assays. CPK-BB measurements were performed by electrophoresis in agarose gel. CPK-BB remained unchanged in Group I during the course of the study (median range 17 to 20 IU) showing no significant difference from normal levels. Significant elevation of CPK-BB occurred three hours after establishing the ischemia in Group II (median 50 IU) as compared to Group I (p < 0.05). The highest levels were deter-mined to be 96 and 80 IU at the sixth and ninth hour, respectively. From hour 15 until completion of the experiment, no significant difference was detected between Groups I and II. We conclude that CPK-BB elevation in serum signifies its release from the smooth muscles of the bowel due to ischemic cellular damage.

Key words

Creatine Phosphokinase CPK-BB isoenzyme Intestinal ischemia, experimental 

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Copyright information

© Annals of Vascular Surgery Inc. 1989

Authors and Affiliations

  • Vassilios E. Smirniotis
    • 1
  • Alexandra T. Labrou
    • 1
  • Dimitris D. Tsiftses
    • 1
  1. 1.Medical School, Second Department of Surgery, Areteion HospitalAthens UniversityAthensGreece

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