Skip to main content
SpringerLink
Log in

Acellular vascular matrix: A natural endothelial cell substrate

  • Third Symposium on Endothelial Seeding
  • Published:
Annals of Vascular Surgery

Abstract

A preliminary assessment was made of the acellular vascular matrix graft as a substrate for endothelial cell seeding, with respect to surface pretreatment (none versus fibronectin and/or serum) and presence of exogenous growth factor. Arteries were harvested from greyhounds and exposed to a sequential detergent extraction process to produce the acellular vascular matrix. Human umbilical vein endothelial cells were grown in tissue culture, harvested in first passage, then seeded at 10s cells/cm2 on sections of acellular vascular matrix and on gel-coated polystyrene positive controls. After 18 hour incubation, endothelial cell-seeded acellular matrices were fixed and processed for histologic and planimetric analysis; control wells were fixed and endothelial cells were counted by planimetry. Pretreatment of the acellular vascular matrix was found to have no effect on the percentage of endothelial cell coverage of the matrix. There was significantly better endothelial cell coverage of the acellular matrix than on matched gel-treated polystyrene control wells. Withdrawal of growth factor resulted in a significant reduction in endothelial cell coverage for all acellular vascular matrix groups. Growth factor withdrawal also significantly reduced attachment of endothelial cells on gel-treated polystyrene. Cell surface area was significantly smaller when growth factor was withdrawn from all groups except from the acellular vascular matrix without pretreatment. We conclude that: (1) the acellular vascular matrix is conducive to endothelial cell adherence and spreading even without pretreatment; and (2) sudden withdrawal of exogenous growth factor may impair early coverage of substrates by endothelial cells due to an effect on the;r adherence or spreading.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. KESLER KA, HERRING MB, ARNOLD MP, GLOVER JL, PARK HM, ELMUS MN, BENDICK PJ. Enhanced strength of endothelial attachment on polyester elastomer and polytetrafluoroethylene graft surfaces with fibronectin substrate.J Vasc Surg 1986;3:8–64.

    Article  Google Scholar 

  2. RAMALANJAONA G, KEMPCZINSKI RF, ROSENMAN JE, DOUVILLE EC, SILBERSTEIN EB. The effect of fibronectin coating on endothelial cell kinetics in polytetra-fluoroethylene grafts.J Vasc Surg 1986;3:272.

    Article  Google Scholar 

  3. KLEINMAN HK, KLEBE RJ, MARTIN GR. Role of collagenous matrices in the adhesion and growth of cells.J Cell Biol 1981;88:473–485.

    Article  CAS  PubMed  Google Scholar 

  4. GORDON PB, LEVITT MA, JENKINS CSP, HATCHER VB. The effect of the extracellular matrix on the detachment of human endothelial cells.J Cell Physiol 1984;121:467–475.

    Article  CAS  PubMed  Google Scholar 

  5. GOSPODAROWICZ D, ILL C. Extracellular matrix and control of proliferation of vascular endothelial cells.J Clin Invest 1980;65:1351–1364.

    Article  CAS  PubMed  Google Scholar 

  6. MADRI JA. Endothelial cell-matrix interactions in hemostasis.Prog Heino Thromb 1982;1–24.

  7. PRATT BM, HARRIS AS, MAORROW JS, MADRI JA. Mechanisms of cytoskeletal regulation.Am J Pathol 1984;117:349–354.

    CAS  PubMed  Google Scholar 

  8. MALONE JM, BRENDEL K, DUHAMEL RC, REINERT RL. Detergent-extracted small diameter vascular prostheses.J Vasc Surg 1984;1:181–191.

    Article  CAS  PubMed  Google Scholar 

  9. DUHAMEL RC, BRENDEL K, REINERT RL, MALONE JM. Properties of acellular vascular matrix. Proceedings, Society for Biomaterials, 10th Annual Meeting, April 27–May 1, 1984.

  10. MALONE JM, REINERT RL, BRENDEL K, DUHAMEL RC. Thrombogenicity of small diameter vascular grafts. Proceedings, Society of Biomaterials, 10th Annual Meeting, April 27–May 1, 1984.

  11. DUHAMEL RC, MALONE JM, REINERT RL, BRENDEL K. The effect of glutaraldehyde crosslinking on endothelialization of acellular vascular matrix grafts. Proceedings, Society for Biomaterials, 11th Annual Meeting, April 25–28, 1985.

  12. BERGER K, SAUVAGE IR, RAO AM, WOOD SJ. Healing of arterial prostheses in man: its completeness.Ann Surg 1972;175:118–127.

    Article  CAS  PubMed  Google Scholar 

  13. SAUVAGE LR, BERGER KE, WOOD SJ, YATES SG, SMITH JC, MANSFIELD PB. Interspecies healing of porous arterial prostheses.Arch Surg 1974;109:698–705.

    CAS  PubMed  Google Scholar 

  14. SAUVAGE LR, BERGER K, BEILIN LB, SMITH JC, WOOD SJ, MANSFIELD PB. Presence of endothelium in an axillary-femoral graft of knitted Dacron with an external velour surface.Ann Surg 1975;182:749–753.

    Article  CAS  PubMed  Google Scholar 

  15. GAJDUSEK CM, SCHWARTZ SM. Ability of endothelial cells to condition culture medium.Cell Physiol 1982;110:35–42.

    Article  CAS  Google Scholar 

  16. LIMANNI A, FLEMING T, MOLINA R, HUFNAGEL H, CUNNINGHAM RE, CRUESS DF, SHAREFKIN JB. Expression of genes for platelet-derived growth factor in adult human venous endothelium.J Vasc Surg 1988;7:10–20

    Article  CAS  PubMed  Google Scholar 

  17. HERRING M, EVANS D, BAUGHMAN S, GLOVER J. The quantitation of cultured cellular surface coverage: applications for transparent and opaque surfaces.J Biomed Maler Res 1984;18:567–575.

    Article  CAS  Google Scholar 

  18. CALLOW AD. Presidential address: The microcosm of the arterial wall— A plea for research.J Vasc Surg 1987;5:1–18.

    Article  CAS  PubMed  Google Scholar 

  19. HERRING M, GARDNER A, GLOVER J. A single-staged technique for seeding vascular grafts with autogenous endothelium.Surgery 1978;84:498–504.

    CAS  PubMed  Google Scholar 

  20. CALLOW AD. Endothelial cell seeding: problems and expectations.J Vasc Surg 1987;6:318–319.

    Article  CAS  PubMed  Google Scholar 

  21. ROSENMAN JE, KEMPCZINSKI RF, PEARCE WH, SILBERSTEIN EB. Kinetics of endothelial cell seeding.J Vasc Surg 1985;2:778–784.

    Article  CAS  PubMed  Google Scholar 

  22. BAKER KS, WILLIAMS SK, JARRELL BE, KOOLPE EA, LEVINE E. Endothelialization of human collagen surfaces with human adult endothelial cells.Am J Surg 1985;150:197–200.

    Article  CAS  PubMed  Google Scholar 

  23. WILLIAMS SK, JARRELL BE, FRIEND L, RADOMSKI JS, CARABASI RA, KOOLPE E, MUELLER SN, THORNTON SC, MARRINUCCI T, LEVINE E. Adult human endothelial cell compatibility with prosthetic graft material.J Surg Res 1985;38:618–629.

    Article  CAS  PubMed  Google Scholar 

  24. FOXALL TZ, AUGER KR, CALLOW AD, LIBBY P. Adult human endothelial cell coverage of small-caliber Dacron and polytetrafluoroethylene vascular prostheses in vitro.Surg Res 1986;41:pp 158–172.

    Article  CAS  Google Scholar 

  25. HAYASHI I, SATO GH. Replacement of serum by hormones permits growth of cells in a defined medium.Nature 1976;259:132–134.

    Article  CAS  PubMed  Google Scholar 

  26. KAN M, KATO M, YAMANE I. Fibroblast products adsorb to the culture substrate and stimulate growth of human umbilical vein endothelial cells.J Cell Physiol 1985;124:125–130.

    Article  CAS  PubMed  Google Scholar 

  27. VLODAVSKY I, SULLIVAN R, FRIDMAN R, SASSE J, FOLKMAN J, KALGSBRUN M. Heparin binding endothelial cell growth factor produced by endothelial cells and sequestered by the subendothelial extracellular matrix.J Cell Biol 1986;103(5) Part 2: 98a (Abst).

    Google Scholar 

  28. FOLKMAN J, KLAGSBRUN M, SASSE J, WADZINSKI M, INGBER D, VLODAVSKY I. A heparin-binding angiogenic protein-basic fibroblast growth factor is stored within basement membrane.Am J Pathol 1988;130:393–399.

    CAS  PubMed  Google Scholar 

  29. GOSPODAROWICZ D, MORAN J, BRAUN D, BIRD-WELL C. Clonal growth of bovine vascular endothelial cells: fibroblast growth factor as a survival agent.Proc Natl Acad SCi USA 1976;73:4120–4124.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Author notes

  1. Stephen G. Lalka MD

    Present address: Wishard Memorial Hospital, Indiana University Medical Center, 1001 West 10th Street, 46202, Indianapolis, Indiana

Authors and Affiliations

  1. Department of Surgery, Indiana University School of Medicine, Indianapolis, Indiana

    Stephen G. Lalka MD, Lisette M. Oelker BS, James M. Malone MD, Raymond C. Duhamel PhD, Melissa A. Kevorkian BS, Beth A. Raper BA, J. Craig Nixon MS, Karen J. Etchberger PhD, Michael C. Dalsing MD, Dolores F. Cikrit MD, Joseph L. Unthank PhD & Malcolm B. Herring MD

  2. Department of Surgery, Maricopa Medical Center, Phoenix, Arizona

    Stephen G. Lalka MD, Lisette M. Oelker BS, James M. Malone MD, Raymond C. Duhamel PhD, Melissa A. Kevorkian BS, Beth A. Raper BA, J. Craig Nixon MS, Karen J. Etchberger PhD, Michael C. Dalsing MD, Dolores F. Cikrit MD, Joseph L. Unthank PhD & Malcolm B. Herring MD

  3. Department of Pharmacology, University of Arizona, Tuscon, Arizona

    Stephen G. Lalka MD, Lisette M. Oelker BS, James M. Malone MD, Raymond C. Duhamel PhD, Melissa A. Kevorkian BS, Beth A. Raper BA, J. Craig Nixon MS, Karen J. Etchberger PhD, Michael C. Dalsing MD, Dolores F. Cikrit MD, Joseph L. Unthank PhD & Malcolm B. Herring MD

  4. Endotech Corporation, Indianapolis, Indiana

    Stephen G. Lalka MD, Lisette M. Oelker BS, James M. Malone MD, Raymond C. Duhamel PhD, Melissa A. Kevorkian BS, Beth A. Raper BA, J. Craig Nixon MS, Karen J. Etchberger PhD, Michael C. Dalsing MD, Dolores F. Cikrit MD, Joseph L. Unthank PhD & Malcolm B. Herring MD

  5. Department of Surgery, St. Vincent Hospital, Indianapolis, Indiana

    Stephen G. Lalka MD, Lisette M. Oelker BS, James M. Malone MD, Raymond C. Duhamel PhD, Melissa A. Kevorkian BS, Beth A. Raper BA, J. Craig Nixon MS, Karen J. Etchberger PhD, Michael C. Dalsing MD, Dolores F. Cikrit MD, Joseph L. Unthank PhD & Malcolm B. Herring MD

Authors
  1. Stephen G. Lalka MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lisette M. Oelker BS
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. James M. Malone MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Raymond C. Duhamel PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Melissa A. Kevorkian BS
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Beth A. Raper BA
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. J. Craig Nixon MS
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Karen J. Etchberger PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Michael C. Dalsing MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Dolores F. Cikrit MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Joseph L. Unthank PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Malcolm B. Herring MD
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Lalka, S.G., Oelker, L.M., Malone, J.M. et al. Acellular vascular matrix: A natural endothelial cell substrate. Annals of Vascular Surgery 3, 108–117 (1989). https://doi.org/10.1007/BF03187377

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03187377

Key words

Search

Navigation