Chinese Science Bulletin

, Volume 46, Issue 6, pp 482–484 | Cite as

Increase of sulphur-containing amino acids in transgenic potato with 10 ku zein gene from maize

  • Lei Li
  • Songmei Liu
  • Yuanlei Hu
  • Weiping Zhao
  • Zhongping Lin


The 10 ku zein gene of maize was under control of patatin class I promoter of potato and transferred into potato genome by the leaf-disc method. The expression of 10 ku zein was determined in the tuber of transgenic plants by RT-PCR. Furthermore, the sulphur-containing amino acids in transgenic tuber increase remarkably.


10 ku zein gene patatin class I promoter sulphur-containing amino acids in potato quality improvement 


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  1. 1.
    Altenbach, S. B., Pearson, K. W., Meeker, G. et al., Enhancement of the methionine content of seed protein by the expression of a chimeric gene encoding a methionine-rich protein in transgenic plants, Plant Mol. Biol., 1989, 13: 513.CrossRefGoogle Scholar
  2. 2.
    Vayda, M. M., Belknap, W. R., The emergence of transgenic potato as commercial products and tool for basic science, Transgenic Res., 1992, 1: 149.CrossRefGoogle Scholar
  3. 3.
    Ardos, P., Pedersen, K., Marks, M. D. et al., A Structural model for maize zein proteins, J. Biol. Chem., 1982, 257(17): 9984.Google Scholar
  4. 4.
    Lee, K. M., Jones, R. A., Dabby, A. et al., Genetic regulation of storage protein content in maize endosperm, Biochem. Gen., 1976, 14: 641.CrossRefGoogle Scholar
  5. 5.
    Pedersen, K., Argos, P. Naravana, S. V. et al., Sequence analysis and characterization of a high sulphur zein protein of Mr 15 000, J. Biol. Chem., 1986, 261: 6279.Google Scholar
  6. 6.
    Kirihara, J. A., Petri, J. B., Messing, J., Isolation and sequence of a gene encoding a methionine-rich 10-kDa zein protein from maize, Gene, 1988, 71: 359.CrossRefGoogle Scholar
  7. 7.
    Bagga, S., Adams, H., Kemp, J. D. et al., Accumulation of 15-kilodalton zein in novel protein bodies in transgenic tobacco, Plant Physiol., 1995, 107: 13.Google Scholar
  8. 8.
    Bagga, S., Adams, H. P., Rodriguez, F. D. et al., Coexpression of the maize δ-zein and β-zein genes results in stable accumulation of δ-zein in endoplasmic reticulum-derived protein bodies formed by β-zein, The Plant Cell, 1997, 9: 1683.CrossRefGoogle Scholar
  9. 9.
    Saghai-Maroof, M. A., Soliman, K. M., Jorgensen, R. A. et al., Ribosomal DNA spacer length polymorphisms in barley: mendelian inheritance, chromosomal location and population dynamics, Proc. Natl. Acad. Sci., 1986, 81: 8014.CrossRefGoogle Scholar
  10. 10.
    Roy, P., Sahasradbudhe, N., A sensitive and simple paper chromatographic procedure for detecting neomycin phospho transferase II (NPT II) gene expression, Plant Mol. Biol., 1990, 14: 873.CrossRefGoogle Scholar
  11. 11.
    Jefferson, R., Goldsbrough, A., Bevan, M., Transcriptional regulation of a patatin-1 gene in potato, Plant Mol. Biol., 1990, 14: 995.CrossRefGoogle Scholar
  12. 12.
    Twell, D., Ooms, G., Structural diversity of the patatin gene family in potatocv. Desiree, Mol. Gen. Genet, 1988, 12: 325.CrossRefGoogle Scholar
  13. 13.
    Pikaard, C. S., Brusca, J. S., Hannapel, D. J. et al., The two classes of genes for the major potato tuber protein, patatin, are differentially expressed in tubers and roots, Nucleic. Acids. Res., 1987, 15: 1979.CrossRefGoogle Scholar

Copyright information

© Science in China Press 2001

Authors and Affiliations

  • Lei Li
    • 1
  • Songmei Liu
    • 2
  • Yuanlei Hu
    • 1
  • Weiping Zhao
  • Zhongping Lin
    • 1
  1. 1.National Laboratory of Protein Engineering and Plant Genetic EngineeringPeking UniversityBeijingChina
  2. 2.College of BiologyBeijing Forest UniversityBeijingChina

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