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Inheritance and expression of multiple disease and insect resistance genes in transgenic rice

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Chinese Science Bulletin

Abstract

2–3 anti-fungal disease genes are coinserted with hygromycin phosphotransferase in the same vector. Two insecticidal genes and PPT acetyl transferase genes are placed in another one. The vectors are co-delivered to rice embryonic cellus tissue at a molar ratio of 1 : 1 using the particle gun method. 55 independent regenerated lines have been obtained through screening for hygromycin resistance. Of these, 70% transgenic plants harbor 6–7 foreign genes. The genes on the same vectors are always co-delivered to rice plant. Northern blot analysis has indicated that the multiple foreign genes give stable expression. In the 6 transgenic plants carrying 6–7 foreign genes, multiple foreign genes tend to integrate in 1 or 2 genetic loci. Progeny segregation is consistent with Mendel’s 3 : 1 segregation law. 8 homozygous R1 transgenic plants harboring 2–3 anti-fungal and 2 insecticidal genes are selected from large number of transgenic progeny screening for hygromycin and Basta resistance.

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Authors and Affiliations

  1. Biotechnology Research Center, Zhongshan University, 510275, Guangzhou, China

    Daorong Feng, Xinping Xu, Guohua Qiu & Baojian Li

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  1. Daorong Feng
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  2. Xinping Xu
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  3. Guohua Qiu
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  4. Baojian Li
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Correspondence to Baojian Li.

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Feng, D., Xu, X., Qiu, G. et al. Inheritance and expression of multiple disease and insect resistance genes in transgenic rice. Chin.Sci.Bull. 46, 101–106 (2001). https://doi.org/10.1007/BF03187001

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  • DOI: https://doi.org/10.1007/BF03187001

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