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Proceedings: Animal Sciences

, Volume 92, Issue 2, pp 121–128 | Cite as

Histochemical distribution of tetrazolium reductases, dehydrogenases and lipids in the follicular wall of normal and atretic follicles in the ovary of the Indian gerbilTatera indica (Muridae: Rodentia)

  • V R Parshad
  • S S Guraya
Article

Abstract

The comparison of the histochemical features of the follicular wall in normal and atretic follicles of corresponding stages in the ovaries of Indian gerbilTatera indica revealed conspicuous changes in amount of lipids and activities of NADH- and NADPH tetrazolium reductases, δ5-3β-hydroxysteroid dehydrogenase and 17α-hydroxysteroid dehydrogenase and succinate- and lactate dehydrogenases in the granulosa layers during follicular growth and atresia. The thecal layers showed a progressive increase in their amounts during follicular growth and these enzymes are not affected significantly by atresia. Atresia resulted in increased amounts of lipids in the granulosa cells and decreased activities of succinate- and lactate dehydrogenases and NADH- and NADPH tetrazolium reductases during latter stages of degeneration.

Keywords

Atresia dehydrogenase follicle granulosa lipids NADH- and NADPH-tetrazolium reductases thecal layer Tatera indica 

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References

  1. Albertini D F 1980 Structural modifications of granulosa cell plasma membrane during folliculogenesis. InBiology of the ovary (eds) P M Motta and E S E Hafez (The Hague, Boston and London; Martinus Nijhoff Publishers) 138–149Google Scholar
  2. Bjersing L 1978 Maturation, morphology and endocrine function of the follicular wall in mammals. InThe vertebrate ovary (ed) R E Jones (New York and London: Plenum Press) 181–214Google Scholar
  3. Bohr J, Gardner R, Schenck P and Shahabi N 1980 Follicular steroidogenesis: Effects of reproductive condition;Biol. Rep. 22 817–826CrossRefGoogle Scholar
  4. Byskov A G 1978 Follicular atresia. InThe vertebrate ovary (ed) R E Jones (New York and London: Plenum Press) 533–562Google Scholar
  5. Dimino M J and Campbell M D 1980 The function and differentiation of ovarian mitochondria. InBiology of the ovary (eds) P M Motta and E S E Hafez (The Hague, Boston and London: Martinus Nijhoff Publishers) 191–195Google Scholar
  6. Fortune J E and Armstrong D T 1977 Androgen production by theca and granulosa isolated from rat follicles;Endocrinology 100 1341–1347CrossRefPubMedGoogle Scholar
  7. Guraya S S 1973a Morphology, histochemistry and biochemistry of follicular growth and atresia;Ann. Biol. Anim. Biochim. Biophys. 13 Suppl. 229–240CrossRefGoogle Scholar
  8. Guraya S S 1973b Follicular atresia;Proc. Indian Natl. Sci. Acad. B39 311–332Google Scholar
  9. Guraya S S 1980 Histochemistry of the ovary. InBiology of the ovary (eds) P M Motta and E S E Hafez (The Hague, Boston and London: Martinus Nijhoff Publishers) 33–51 (London: Churchill) 759Google Scholar
  10. Guraya S S and Greenwald G S 1964a A comparative histochemical study of interstitial tissue and follicular atresia in the mammalian ovary;Anat. Rec. 149 411–454CrossRefPubMedGoogle Scholar
  11. Guraya S S and Greenwald G S 1964b Histochemical studies on the interstitial gland in the rabbit ovary;Am. J. Anat. 114 495–520CrossRefPubMedGoogle Scholar
  12. Guraya S S and Gupta S K 1979 Morphology and histochemistry of ovarian changes in the field rat,Millardia meltada;Z. Mikrosk. Anat. Forsch. (Leipzig) 93 959–973Google Scholar
  13. Hoyer P E 1980 Histoenzymology of the human ovary: Dehydrogenases directly involved in steroidogenesis. InBiology of the ovary (eds) P M Motta and E S E Hafez (The Hague, Boston and London: Martinus Nijhoff Publishers) 52–67Google Scholar
  14. Kingge K M and Leathern J H 1956 Growth and atresia in the ovary of the hamster;Anat. Rec. 124 679–698CrossRefGoogle Scholar
  15. Lojda Z, Gossrau R and Schieber T H 1979Enzyme histochemistry; (Berlin: Springer Verlag) 339Google Scholar
  16. Motta P 1972 Histochemicai evidence of early stages of atretic follicles in different mammals;J. Cell Biol. 55 182aGoogle Scholar
  17. Pearse AGE 1968Histochemistry, theoretical and applied histochemistry, (London: Churchill) 759Google Scholar
  18. Pupkin M, Bratt H, Weisz J, Lloyd C W and Balogh K Jr 1966 Dehydrogenases in the rat ovary. I. A histochemicai study of Δ5-3β- and 20α-toydroxysteroid dehydrogenases and enzymes of carbohydrate oxidation during estrous cycle;Endocrinology 79 316–327CrossRefPubMedGoogle Scholar

Copyright information

© Indian Academy of Sciences 1983

Authors and Affiliations

  • V R Parshad
    • 1
  • S S Guraya
    • 1
  1. 1.Department of ZoologyPunjab Agricultural UniversityLudhianaIndia

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