Proceedings: Animal Sciences

, Volume 90, Issue 5, pp 495–501 | Cite as

Effect of castration on some hydrolytic enzymes in the rat vas deferens: A histochemical study

  • S S Guraya
  • Arbans J Kaur


A histochemical study has been made of alterations in some hydrolytic enzymes such as alkaline phosphatase, acid phosphatase and 5’-nucleotidase of the vas deferens of sexually mature albino rats following bilateral testectomy. Three weeks after operation, vas deferens shows a significant decrease in the activities of acid phosphatase, alkaline phosphatase and 5’-nucleotidase indicating conspicuous metabolic alterations and reduction in transport mechanisms following removal of the source of androgens.


Rodent vas deferens histochemistry enzymes castration albino rat 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Allen J M and Slater J J 1957 A chemical and histochemical study of alkaline phosphatase and aliesterase in the epididymis of normal and castrated mice;Anat. Rec.129 255–273CrossRefPubMedGoogle Scholar
  2. Allen J M and Slater J J 1958 A chemical and histochemical study of acid phosphatase in the epididymis of normal, castrate and hormone replaced castrate mice;Anat. Rec. 130 731–745CrossRefPubMedGoogle Scholar
  3. Brooks D E 1976 Activity and androgenic control of glycolytic enzymes in the epididymis and epididymal spermatozoa of rat;Biochem. J. 156 527–537PubMedGoogle Scholar
  4. Brooks D E 1978 Activity and androgenic control of enzymes associated with the tricarboxylic acid cycle, lipid oxidation and mitochondrial shuttles in the epididymis and epididymal spermatozoa of the rat;Biochem. J. 174 741–752PubMedGoogle Scholar
  5. Dawson R M C and Rowlands I W 1959 Glycerophosphorycholine in the male reproductive organs of rats and guinea pigs;Q. J. Exptl. Physiol 44 26–34Google Scholar
  6. Flickinger C J 1973 Regional variations in endoplasmic reticulum in the vas deferens of normal and vasectomized rats;Anat. Rec. 176 205–223CrossRefPubMedGoogle Scholar
  7. Glover T D 1960 Spermatozoa from the isolated cauda epididymides of rabbits and rams; effect of artificial cryptorchidism;J. Reprod. Fertil. 1 121–129CrossRefGoogle Scholar
  8. Gregoire A T, Moran M J and Cuadros A 1973 Activity and localization of isocitric dehydrogenase, aspartate amino-transferase, alanine aminotransferase, alkaline and acid phosphatase of the human vas deferens;Fertility Sterility 24 309–313Google Scholar
  9. Hoffer A P 1976 The ultrastructure of the ductus deferens in man;Biol. Reprod. 14 435–443CrossRefGoogle Scholar
  10. Lubicz-Nawrocki C and Glover T D 1970 Effect of gonadectomy and testosterone replacement on the viability of epididymal spermatozoa in the golden hamster (Mesacriceus auratiis);J Endocrinol.48 xxiiGoogle Scholar
  11. Mainwaring W I P 1977 The mechanism of action of androgens; in monographs on endocrinology (eds.) F Gross, M M Grumbach, A Labhart, M B Lipsett, T Mann, L T Samuels and J Zander (New York: Springer-Verlag)10 1–178Google Scholar
  12. Martan J and Risley PLC 1963 Holocrine secretory cells of the rat epididymis;Anat. Rec. 146 173–189CrossRefGoogle Scholar
  13. Martan J 1969 Epididymal histochemistry and physiology;Biol. Reprod. Suppl. 1 134–154CrossRefGoogle Scholar
  14. Niemi M 1965 The fine structure and histochemistry of the epithelial cells of the rat vas deferens;Acta Anat. 60 207–219.CrossRefPubMedGoogle Scholar
  15. Nun S, Musacchio I and Epstein J A 1972 Variations in seminal plasma constituents from fertile, subfertile and vasectomized azoospermic men;Fertil. Steril. 23 357–360PubMedGoogle Scholar
  16. Pearse A G E 1968Histochemistry, theoretical and applied (London: J and A Churchill Ltd.) pp. 710–729Google Scholar
  17. Sholl S A and Leathern J H 1973 Effects of postnatal maturation and castration on rat epididymal carbohydrate metabolism (37083);Proc. Soc. Exp. Biol. Med. 142 635–637PubMedGoogle Scholar

Copyright information

© Indian Academy of Sciences 1981

Authors and Affiliations

  • S S Guraya
    • 1
  • Arbans J Kaur
    • 1
  1. 1.Department of Zoology, College of Basic Sciences and HumanitiesPunjab Agricultural UniversityLudhianaIndia

Personalised recommendations