Protective effects of caffeic acid phenethyl ester on iron-induced liver damage in rats
Caffeic acid phenethyl ester (CAPE) is a natural product with potent anti-inflammatory, antitumor, and antioxidant activities, and attenuates inflammation and lipid peroxidation. The purpose of the present study was to investigate the effects of CAPE on iron-induced liver damage. Rats were divided into four groups and treated for 7 days with saline (control group), 10 µmol kg CAPE/day s.c. (CAPE group), 50 mg iron-dextran/kg i.p. (IRON group) and CAPE and iron at the same time (IRON+CAPE group). Seven days later, rats were killed and the livers were excised for biochemical analysis. The administration of IRON alone resulted in higher myeloperoxidase (MPO) activity and lipid peroxidation than in the control and CAPE treatment prevented the increase in MPO activity and malondialdeyde (MDA) level. No differences were observed in all four groups with regards to superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities. Our results collectively suggest that CAPE may be an available agent to protect the liver from injury via inhibition of MPO activity.
KeywordsCaffeic acid phenethyl ester Iron overload Myeloperoxidase Malondialdehyde Oxidative stress Liver
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- 4.Britton, R. (2000): “Hemochromatosis: Genetics, Pathophysiology, Diagnosis and Treatment”. Cambridge University Press, New York.Google Scholar
- 17.Aebi, H. (1974): “Methods of Enzymatic Analysis”. Academic Press, New York.Google Scholar
- 19.Pardo-Andreu, G.L., Barrios, M.F., Curti, C., Hernandez, I., Merino, N., Lemus, Y., Martinez, I., Riano, A., and Delgado, R. (2008): Protective effects of Mangifera indica L extract (Vimang), and its major component mangiferin, on ironinduced oxidative damage to rat serum and liver. Pharmacol Res, 57, 79–86.CrossRefPubMedGoogle Scholar
- 21.Livrea, M.A, Tesoriere, L., Pintaudi, A.M., Calabrese, A., Maggio, A., Freisleben, H.,, D’Arpa, D., D’Anna, R., and Bongiorno, A. (1996): Oxidative stress and antioxidant status in beta-thalassemia major: iron overload and depletion of lipid-soluble antioxidants. Blood, 88, 3608–3614.PubMedGoogle Scholar
- 28.Tan, J., Ma, Z., Han, L., Du, R., Zhao, L., Wei, X., Hou, D., Johnstone, B.H., Farlow, M.R., and Du, Y. (2005): Caffeic acid phenethyl ester possesses potent cardioprotective effects in a rabbit model of acute myocardial ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol, 289, H2265–2271.CrossRefPubMedGoogle Scholar