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Chinese Science Bulletin

, Volume 49, Issue 20, pp 2144–2147 | Cite as

Reaction dynamics between the photonuclease triplet-excited vitamin-K3 and guanine nucleotide

  • Huijuan Zhang
  • Manyu Li
  • Peng Wang
  • Juan Feng
  • Li Wang
  • Xicheng Ai
  • Xingkang Zhang
  • Jianping Zhang
Articles

Abstract

Nanosecond laser pulse photolysis was used to investigate the excited-state dynamics of the photonuclease vitamin-K3 (VK3). A spectral band peaked at 285 nm was newly observed and attributed to the triplet-excited state of VK3. In studying the photochemistry between the photonuclease VK3 and guanine nucleotide (dGMP), we obtained spectroscopic evidence for the photo-initiated formation of both VK3 radical anion and dGMP radical cation, and thus proved the charge transfer reaction between the triplet-excited VK3 and the ground-state dGMP.

Keywords

vitamin-K3 triplet-state guanine nucleotide charge transfer 

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References

  1. 1.
    Thomson, R. H., Naturally Occurring Quinones, Vol. III, London: Chapman and Hall, 1987.Google Scholar
  2. 2.
    Powis, G., Metabolism and reactions of quinonoid anticancer agents, Pharmacol. Ther., 1987, 35: 57–162.CrossRefGoogle Scholar
  3. 3.
    Qu, X., Wan, C., Becker, H. C. et al., The anticancer drug-DNA complex: Femtosecond primary dynamics for anthracycline antibiotics function, Proceedings of the National Academy of Sciences of the United States of America, 2001, 98(25): 14212–14217.CrossRefGoogle Scholar
  4. 4.
    Adams, G. E., Hypoxic cell sensitizers for radiotherapy, Cancer, a Comprehensive Treatise (ed. Becker, F. F.), New York: Plenum Press, 1977, 6: 181–223.Google Scholar
  5. 5.
    Wagner, J. R., Cadet, J., Fisher, G. J., Photo-oxidation of thymine sensitized by 2-methyl-1, 4-naphthoquinone: analysis of products including three novel photo-dimers, Photochemistry and Photobiology, 1984, 40(5): 589–597.CrossRefGoogle Scholar
  6. 6.
    Wagner, J. R., Lier, J. E. V., Johnston, L. J., Quinone sensitized electron transfer photooxidation of nucleic acids: Chemistry of thymine and thymidine radical cations in aqueous solution, Photochemistry and Photobiology, 1990, 52(2): 333–343.CrossRefGoogle Scholar
  7. 7.
    Melvin, T., Bothe, E., Frohlinde, D. S., The reaction of triplet 2-methyl-1, 4-naphthoquinone (menadione) with DNA and polynucleotides, Photochemistry and Photobiology, 1996, 64(5): 769–776.CrossRefGoogle Scholar
  8. 8.
    Zhang, L., Lin, W., Yao, S. et al., DNA photosensitization damage induced by 2-methyl-1, 4-naphthoninone, Photographic Science and Photochemistry, 2002, 20(5): 352–357.Google Scholar
  9. 9.
    Ma, J., Lin, W., Wang, W. et al., Triplet-state mechanism for electron transfer oxidation of DNA, Journal of Photochemistry and Photobiology B: Biology, 2000, 57: 76–81.CrossRefGoogle Scholar
  10. 10.
    Giese, B., Amaudrut, J., Köhler, A. K. et al., Direct observation of hole transfer through DNA by hopping between adenine bases and by tunneling, Nature, 2001, 412(19): 318–320.CrossRefGoogle Scholar
  11. 11.
    Seidel, C. A. M., Schulz, A., Sauer, M. H. M., Nucleobase-specific quenching of fluorescence dyes, Part 1: Nucleobase one-electron redox potentials and their correlation with static and dynamic quenching efficiencies, J. Phys. Chem., 1996, 100(13): 5541–5553.CrossRefGoogle Scholar
  12. 12.
    Fisher, G. J., Land, E. J., Photosensitization of pyrimidines by 2-methylnaphthoquinone in water: A laser flash photolysis study, Photochemistry and Photobiology, 1983, 37(1): 27–32.CrossRefGoogle Scholar
  13. 13.
    Ham, S. W., Song, J. H., Kim, H. I. et al., Mechanism of cell cycle arrest by menadione, Bull. Korean Chem. Soc., 2000, 21(12): 1173–1174.Google Scholar
  14. 14.
    Shafirovich, V., Dourandin, A., Huang, W. et al., Electron transfer at a distance induced by site-selective photoionization of 2-aminopurine in oligonucleotides and investigated by transient absorption techniques, Phys. Chem. Chem. Phys., 2000, 2: 4399–4408.CrossRefGoogle Scholar
  15. 15.
    Dervan, P. B., Burli, R. W., Sequence-specific DNA recognition by polyamides, Curr. Opin. Chem. Biol., 1999, 3: 688–693.CrossRefGoogle Scholar

Copyright information

© Science in China Press 2004

Authors and Affiliations

  • Huijuan Zhang
    • 1
  • Manyu Li
    • 1
  • Peng Wang
    • 1
  • Juan Feng
    • 1
  • Li Wang
    • 2
  • Xicheng Ai
    • 1
  • Xingkang Zhang
    • 1
  • Jianping Zhang
    • 1
  1. 1.State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of ChemistryChinese Academy of SciencesBeijingChina
  2. 2.Center for Condensed Matter Physics, Institute of PhysicsChinese Academy of SciencesBeijingChina

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