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Chinese Science Bulletin

, Volume 49, Issue 15, pp 1606–1610 | Cite as

Identification and function analysis of spectrin-like protein in pollen tubes of lily (Lilium davidii Duch)

  • Xueqin Zhang
  • Ming Yuan
  • Xuechen Wang
Articles
  • 20 Downloads

Abstract

The elongation of pollen tube is an important process of sexual reproduction in higher plant. Cytoskeleton plays a major regulatory role in the elongation of pollen tubes. But whether membrane skeleton is involved in the pollen tube elongation is not clear. In this study, immunochemical detection of spectrin-like protein has been carried out in pollen tubes. By use of 2-dimensional electrophoresis(2DE) and western blotting, two spectrin-like proteins are found, one is 150 kD, and the other is 105 kD, with pI being 4.54 and 4.39, respectively. 150 kD spectrin-like protein is located in plasma membrane of pollen tube and 105 kD spectrin-like protein is located in cytoplasm, probably functioning as a subunit to form a dimmer (210 kD)in vivo. The elongation of pollen tubes is inhibited after spectrin antibody was injected into a growing pollen tube. These results suggest that spectrin-like proteins exist in pollen tube and play an important regulating role in the elongation process of pollen tubes from lily.

Keywords

spectrin microfilament actin-binding-proteins pollen tubes 

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Copyright information

© Science in China Press 2004

Authors and Affiliations

  1. 1.State Key Laboratory of Plant Physiology and Biochemistry, College of Biological SciencesChina Agricultural UniversityBeijingChina

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