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Science in China Series C: Life Sciences

, Volume 48, Issue 3, pp 300–306 | Cite as

Applications of the double-barreled data in whole-genome shotgun sequence assembly and analysis

  • Yujun Han
  • Peixiang Ni
  • Hong Lü
  • Jia Ye
  • Jianfei Hu
  • Chen Chen
  • Xiangang Huang
  • Lijuan Cong
  • Guangyuan Li
  • Jing Wang
  • Xiaocheng Gu
  • Jun Yu
  • Songgang Li
Article
  • 31 Downloads

Abstract

Double-barreled (DB) data have been widely used for the assembly of large genomes. Based on the experience of building the whole-genome working draft ofOryza sativa L. ssp.Indica, we present here the prevailing and improved uses of DB data in the assembly procedure and report on novel applications during the following data-mining processes such as acquiring precise insert fragment information of each clone across the genome, and a new kind of low-cost whole-genome microarray. With the increasing number of organisms being sequenced, we believe that DB data will play an important role both in other assembly procedures and in future genomic studies.

Keywords

genome double-barreled data application whole-genome microarray 

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Copyright information

© Science in China Press 2005

Authors and Affiliations

  • Yujun Han
    • 1
    • 2
  • Peixiang Ni
    • 2
  • Hong Lü
    • 2
  • Jia Ye
    • 3
  • Jianfei Hu
    • 1
  • Chen Chen
    • 2
  • Xiangang Huang
    • 2
  • Lijuan Cong
    • 2
  • Guangyuan Li
    • 2
  • Jing Wang
    • 1
  • Xiaocheng Gu
    • 1
  • Jun Yu
    • 2
  • Songgang Li
    • 1
    • 2
  1. 1.College of Life SciencesPeking UniversityBeijingChina
  2. 2.Beijing Genomics InstituteChinese Academy of SciencesBeijingChina
  3. 3.James D. Watson Institute of Genome SciencesZhejiang UniversityHangzhouChina

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