Science in China Series C: Life Sciences

, Volume 40, Issue 5, pp 524–530 | Cite as

Molecular replacement studies on crystal structure of DesB1-B2 Despentapeptide (B26-B30) insulin

  • Jiasheng Diao
  • Zhuli Wan
  • Wenrui Chang
  • Dongcai Liang


Using the crystal structure of Despentapeptide (B26-B30) insulin (DPI as the search model, the crystal structure of DesB1-B2 Despentapeptide (B26-B30) insulin (DesB1-2 DPI) has been studied by the molecular replacement method. There is one DesB1-2 DPI molecule in each crystallographic asymmetric unit. The cross rotation function search and the translation function search show apparent peaks and thus determine the orientation and position of DesB1-2 DPI molecule in the cell respectively. The subsequent three-dimensional structural rebuilding and refinement of DesB1-2 DPI molecule confirm the results by molecular replacement method.


DesB1-B2 Despentapeptide (B26-B30) insulin molecular replacement method 


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Copyright information

© Science in China Press 1997

Authors and Affiliations

  • Jiasheng Diao
    • 1
  • Zhuli Wan
    • 1
  • Wenrui Chang
    • 1
  • Dongcai Liang
    • 1
  1. 1.State Key Laboratory of Biornacrornolecules, Institute of BiophysicsChinese Academy of SciencesBeijingChina

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